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The map label for this gene is lpdA [H]

Identifier: 33862608

GI number: 33862608

Start: 382161

End: 383630

Strand: Direct

Name: lpdA [H]

Synonym: PMT0335

Alternate gene names: 33862608

Gene position: 382161-383630 (Clockwise)

Preceding gene: 33862607

Following gene: 33862609

Centisome position: 15.85

GC content: 55.03

Gene sequence:

>1470_bases
GTGAGCGAAGCCAGTTTCGACTTCGATGTGATCGTGATCGGTGCGGGTTACGGCGGTTTTGATGCCGCCAAGCATGCCGC
TGATGATGGCCTCAAGGTGGCGATTGTGGAATCAGGTGAGATGGGCGGCACTTGCGTGAATCGAGGCTGTGTGCCCTCTA
AGGCATTGCTTGCTGCTAGTGGAAGGGTGCGTGAGCTGGCTGATGCTGAACACCTATCTGGTTTCGGGATCCATGCTGCA
CCGGTGCGTTTTGAACGCCAGAAGATTGCTGACCATGCCAACCAGTTGGTGGCCACGATTCGTAGCAACCTCACCAAAAC
GCTGCAGCGGGCTGGTGTCACGATCCTGCGTGGCCATGGACGATTGGAGGGAAGCCAGCGAATTGGCTTGAGAGAGAAAA
GTGGAGTGGATCGGCTGCTCACTGCCAGGGATGTGATCCTTGCCACAGGCTCTGATCCATTTGTGCCCCCTGGGATTGAA
ACCGATGGACGCACGGTTTTTACCAGTGATGAAGCCGTGAATTTGGAATGGCTGCCGCGTTGGATTGCAATTATCGGCAG
TGGTTATATCGGCCTTGAATTTGCCGATGTTTATACGGCTCTCGGCTGTGAAGTCACGATGATTGAGGCGTTGGATCGGG
TCATGCCAACCTTTGACCCCGACATCACCAAGATGGCAGCTCGGCATTTGATTGAAGGACGTGACATCGATGCACGTGCT
GGTGTGCTGGCTAGCAAGGTGATCCCTGGCTGCCCGGTAAGGATCGAACTGGCGGAGATGAAAAGCCGTGAGCTGGTGGA
TAGCTTGGAGGTGGATGCGGTGCTCGTCGCTACTGGTCGGGTGCCAAGTAGCAAGGGGCTCAATCTTGAGTCGGTTGGTG
TGGAGACCAATCGTGGCTTTGTGCCGATTGACGACAGCATGCGGGTATTGGTCAACGGAAAGCCGCTGCCTCATCTTTGG
GCCGTCGGTGATGTCACTGGCAAGCTGATGTTGGCCCATACAGCCGCAGCACAGGGCACGCTGGCTGTTGACAACATTCA
GGGACACTCCCGCACGATTGATTACCGCAGTATTCCAGCCGCAACCTTCACTCATCCGGAGATCAGTTCAGTGGGGTTGA
GTGAAGCTGATGCCAAAGATCTTGCCGCCAAGGATGGGTTTGAGTTGGGGAGTGTGCGCAGCTATTTCAAGGCCAATTCC
AAAGCACTAGCAGAGCTGGAGAGTGATGGATTGATGAAGTTGTTGTTCCGCAAAGACAATGGTGAAGTTCTTGGTGCTCA
TATCTATGGATTGCACGCCGCCGATTTGATCCAGGAGGTAGCCAATGCGGTGGCACGACGTCAGAGCGTTGCTCAATTGG
CCACTGAAGTGCATACCCATCCCACCCTGAGTGAAGTGGTGGAAGTGGCCTATAAACAGGCGGCCAAACAGCTGGCTAAG
GCGGTTGTTGCATCGGCCAGTGCTTCCTAG

Upstream 100 bases:

>100_bases
ATAGCGCAGCAGTTGAGTCGCTCAATGTGGCGGCGGTGGCGGTGCCACTACTGCTTGAGCGGCGACGAGCCACAATGACC
TTCTCCACGCAGTAGTTCGG

Downstream 100 bases:

>100_bases
GCCGCTGTTCTTCAAGCAGGTCTTAGCCCTGCGGTTCACTGTTTTTAATTGCACTATCACCCGATGGAGATCCGCAGACG
TCCGCCCAATCCAAGTGTCA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; LPD; E3 component of pyruvate complex [H]

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MSEASFDFDVIVIGAGYGGFDAAKHAADDGLKVAIVESGEMGGTCVNRGCVPSKALLAASGRVRELADAEHLSGFGIHAA
PVRFERQKIADHANQLVATIRSNLTKTLQRAGVTILRGHGRLEGSQRIGLREKSGVDRLLTARDVILATGSDPFVPPGIE
TDGRTVFTSDEAVNLEWLPRWIAIIGSGYIGLEFADVYTALGCEVTMIEALDRVMPTFDPDITKMAARHLIEGRDIDARA
GVLASKVIPGCPVRIELAEMKSRELVDSLEVDAVLVATGRVPSSKGLNLESVGVETNRGFVPIDDSMRVLVNGKPLPHLW
AVGDVTGKLMLAHTAAAQGTLAVDNIQGHSRTIDYRSIPAATFTHPEISSVGLSEADAKDLAAKDGFELGSVRSYFKANS
KALAELESDGLMKLLFRKDNGEVLGAHIYGLHAADLIQEVANAVARRQSVAQLATEVHTHPTLSEVVEVAYKQAAKQLAK
AVVASASAS

Sequences:

>Translated_489_residues
MSEASFDFDVIVIGAGYGGFDAAKHAADDGLKVAIVESGEMGGTCVNRGCVPSKALLAASGRVRELADAEHLSGFGIHAA
PVRFERQKIADHANQLVATIRSNLTKTLQRAGVTILRGHGRLEGSQRIGLREKSGVDRLLTARDVILATGSDPFVPPGIE
TDGRTVFTSDEAVNLEWLPRWIAIIGSGYIGLEFADVYTALGCEVTMIEALDRVMPTFDPDITKMAARHLIEGRDIDARA
GVLASKVIPGCPVRIELAEMKSRELVDSLEVDAVLVATGRVPSSKGLNLESVGVETNRGFVPIDDSMRVLVNGKPLPHLW
AVGDVTGKLMLAHTAAAQGTLAVDNIQGHSRTIDYRSIPAATFTHPEISSVGLSEADAKDLAAKDGFELGSVRSYFKANS
KALAELESDGLMKLLFRKDNGEVLGAHIYGLHAADLIQEVANAVARRQSVAQLATEVHTHPTLSEVVEVAYKQAAKQLAK
AVVASASAS
>Mature_488_residues
SEASFDFDVIVIGAGYGGFDAAKHAADDGLKVAIVESGEMGGTCVNRGCVPSKALLAASGRVRELADAEHLSGFGIHAAP
VRFERQKIADHANQLVATIRSNLTKTLQRAGVTILRGHGRLEGSQRIGLREKSGVDRLLTARDVILATGSDPFVPPGIET
DGRTVFTSDEAVNLEWLPRWIAIIGSGYIGLEFADVYTALGCEVTMIEALDRVMPTFDPDITKMAARHLIEGRDIDARAG
VLASKVIPGCPVRIELAEMKSRELVDSLEVDAVLVATGRVPSSKGLNLESVGVETNRGFVPIDDSMRVLVNGKPLPHLWA
VGDVTGKLMLAHTAAAQGTLAVDNIQGHSRTIDYRSIPAATFTHPEISSVGLSEADAKDLAAKDGFELGSVRSYFKANSK
ALAELESDGLMKLLFRKDNGEVLGAHIYGLHAADLIQEVANAVARRQSVAQLATEVHTHPTLSEVVEVAYKQAAKQLAKA
VVASASAS

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=479, Percent_Identity=32.1503131524008, Blast_Score=242, Evalue=6e-64,
Organism=Homo sapiens, GI50301238, Length=475, Percent_Identity=29.4736842105263, Blast_Score=179, Evalue=4e-45,
Organism=Homo sapiens, GI22035672, Length=466, Percent_Identity=29.8283261802575, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI33519430, Length=488, Percent_Identity=26.844262295082, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI33519428, Length=488, Percent_Identity=26.844262295082, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI33519426, Length=488, Percent_Identity=26.844262295082, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI148277065, Length=488, Percent_Identity=26.844262295082, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI148277071, Length=488, Percent_Identity=26.844262295082, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI291045266, Length=483, Percent_Identity=27.1221532091097, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI291045268, Length=480, Percent_Identity=24.7916666666667, Blast_Score=106, Evalue=6e-23,
Organism=Escherichia coli, GI1786307, Length=464, Percent_Identity=31.8965517241379, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI87082354, Length=478, Percent_Identity=28.8702928870293, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1789915, Length=449, Percent_Identity=28.7305122494432, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI87081717, Length=467, Percent_Identity=26.7665952890792, Blast_Score=126, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI32565766, Length=482, Percent_Identity=32.1576763485477, Blast_Score=218, Evalue=7e-57,
Organism=Caenorhabditis elegans, GI17557007, Length=485, Percent_Identity=28.659793814433, Blast_Score=151, Evalue=9e-37,
Organism=Caenorhabditis elegans, GI71983419, Length=472, Percent_Identity=29.2372881355932, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI71983429, Length=472, Percent_Identity=29.2372881355932, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI71982272, Length=497, Percent_Identity=26.7605633802817, Blast_Score=117, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6321091, Length=482, Percent_Identity=33.195020746888, Blast_Score=214, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6325166, Length=482, Percent_Identity=27.3858921161826, Blast_Score=162, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6325240, Length=487, Percent_Identity=27.7207392197125, Blast_Score=157, Evalue=3e-39,
Organism=Drosophila melanogaster, GI21358499, Length=485, Percent_Identity=32.7835051546392, Blast_Score=238, Evalue=6e-63,
Organism=Drosophila melanogaster, GI24640549, Length=497, Percent_Identity=28.5714285714286, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24640553, Length=495, Percent_Identity=28.6868686868687, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24640551, Length=495, Percent_Identity=28.8888888888889, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI17737741, Length=496, Percent_Identity=27.4193548387097, Blast_Score=129, Evalue=4e-30,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 51951; Mature: 51820

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEASFDFDVIVIGAGYGGFDAAKHAADDGLKVAIVESGEMGGTCVNRGCVPSKALLAAS
CCCCCCCEEEEEEECCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHCC
GRVRELADAEHLSGFGIHAAPVRFERQKIADHANQLVATIRSNLTKTLQRAGVTILRGHG
CCHHHHHCHHHCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCC
RLEGSQRIGLREKSGVDRLLTARDVILATGSDPFVPPGIETDGRTVFTSDEAVNLEWLPR
CCCCCCCCCCCCCCCHHHHHHHCEEEEECCCCCCCCCCCCCCCCEEEECCCEECHHHHHH
WIAIIGSGYIGLEFADVYTALGCEVTMIEALDRVMPTFDPDITKMAARHLIEGRDIDARA
HHHHHCCCCCEEEHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHH
GVLASKVIPGCPVRIELAEMKSRELVDSLEVDAVLVATGRVPSSKGLNLESVGVETNRGF
HHHHHHCCCCCCEEEEHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEECEECCCCE
VPIDDSMRVLVNGKPLPHLWAVGDVTGKLMLAHTAAAQGTLAVDNIQGHSRTIDYRSIPA
EECCCCCEEEECCCCCCCEEEECCCCCEEEEEEHHCCCCCEEEECCCCCCEEECCCCCCC
ATFTHPEISSVGLSEADAKDLAAKDGFELGSVRSYFKANSKALAELESDGLMKLLFRKDN
CCCCCCCHHHCCCCCCCHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHCCCEEEEEEECCC
GEVLGAHIYGLHAADLIQEVANAVARRQSVAQLATEVHTHPTLSEVVEVAYKQAAKQLAK
CCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
AVVASASAS
HHHHHCCCC
>Mature Secondary Structure 
SEASFDFDVIVIGAGYGGFDAAKHAADDGLKVAIVESGEMGGTCVNRGCVPSKALLAAS
CCCCCCEEEEEEECCCCCHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHCC
GRVRELADAEHLSGFGIHAAPVRFERQKIADHANQLVATIRSNLTKTLQRAGVTILRGHG
CCHHHHHCHHHCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCC
RLEGSQRIGLREKSGVDRLLTARDVILATGSDPFVPPGIETDGRTVFTSDEAVNLEWLPR
CCCCCCCCCCCCCCCHHHHHHHCEEEEECCCCCCCCCCCCCCCCEEEECCCEECHHHHHH
WIAIIGSGYIGLEFADVYTALGCEVTMIEALDRVMPTFDPDITKMAARHLIEGRDIDARA
HHHHHCCCCCEEEHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHH
GVLASKVIPGCPVRIELAEMKSRELVDSLEVDAVLVATGRVPSSKGLNLESVGVETNRGF
HHHHHHCCCCCCEEEEHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEEEECEECCCCE
VPIDDSMRVLVNGKPLPHLWAVGDVTGKLMLAHTAAAQGTLAVDNIQGHSRTIDYRSIPA
EECCCCCEEEECCCCCCCEEEECCCCCEEEEEEHHCCCCCEEEECCCCCCEEECCCCCCC
ATFTHPEISSVGLSEADAKDLAAKDGFELGSVRSYFKANSKALAELESDGLMKLLFRKDN
CCCCCCCHHHCCCCCCCHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHCCCEEEEEEECCC
GEVLGAHIYGLHAADLIQEVANAVARRQSVAQLATEVHTHPTLSEVVEVAYKQAAKQLAK
CCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
AVVASASAS
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905231; 9387233 [H]