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The map label for this gene is folC [H]

Identifier: 33862603

GI number: 33862603

Start: 376928

End: 378169

Strand: Reverse

Name: folC [H]

Synonym: PMT0330

Alternate gene names: 33862603

Gene position: 378169-376928 (Counterclockwise)

Preceding gene: 33862604

Following gene: 33862602

Centisome position: 15.69

GC content: 55.64

Gene sequence:

>1242_bases
GTGTCCAAACCCACAAGTCCAAGCAAGGAAGATTTAAGCGATCTGATTCCTCGTTTTGATCAGCGGGGGATGGATCTTGA
TCTTGAGCGCATGCAACAAGCACTGCAGGCCATGGGAAACCCCTGTGCCTCTATTCCAGCCATTCAGGTCGTAGGGACCA
ATGGCAAAGGGTCTATCGCCAGCTTCATTGCCAGCAGCCTTAAGGCTGCTGGCATTCGCGTGGGGCTGACCACCTCTCCT
CACCTCGTGAGCTGGTGTGAACGGATCAGCAGCGATGGTGAGCCGATCTCAATCGTTGAGCTACGCCAACGACTCACCGC
CCTTCAACCCCTTGCCCAAACCCACCGACTCACCCCCTTCGAGCTGTTGATTGCCACGGCTTTTGATCACTTCAGATCCC
GCGAGGTAGAGCTGCTTGTGCTTGAGGTAGGCCTCGGAGGACGCCTCGATGCCACCACAGCTCATCCATACAGACCAATC
ATTGCCATGGCCAACATCGGCCTCGACCACTGCGAACACCTTGGTTACAGCCTTAAAGAGATAACAGCAGAAAAAAGCGC
CGTCATCAGTCCTGGGGCTGCTGTGATCAGTGCTCGTCAACAAACTGAAGTGGCAAGCATTCTTGAAGACAAGGCAAAAC
ATCAACAGGCCCGCTTGCAATGGGTTTCACCCCTCCCAGAGAACTGGGACTTGGGACTGCCAGGAGTCTTGCAACGGCAG
AACGCGGCGGTAGCCAAGGGTGCTCTTGAATCCCTGGCCCCACTTGGCTGGCGACTCAATCAAGACGTCATTCGCACAGG
GCTGGCCCACGCCTACTGGCCTGCACGACTGCAGACCGTTCACTGGCAAAGCCAGCCAGTGCTAATTGATGGTGCCCATA
ACCCACCAGCAGCCGAACGGTTGGCCCACGAGCGGCAGCACTGGAGCAATCAAGAGTTTGGGGTTTGCTGGGTGCTTGGT
CTACAGGCCCACAAGCAAGCACCAGCCATGCTTCGCCATTTACTCAAGCCAAGCGATCTGGCTTGGATCGTGCCGGTGCC
GGAACACTGCAGCTGGACGCAGCACCAACTCGCAGCACACTGCCCCGACCTATCAAGCCAACTCCAATCTGCTGACAATG
TTGAACAGGTCTTCTCCATCCTGCTCAAACAGAATCGATGGCCAAATCCACCTCCAGTTGTAGCTGGTTCCCTCTATTTA
TTAGGTGATCTTCTCGCAAAGCAAACCATCCAGGCAGAGTGA

Upstream 100 bases:

>100_bases
TCCAAAGGTTGTGCGCATGGTGCCGCCGCTGATCATCAAACCAAGCGAAATCCGCCAACTGCTCCAACGTCTGGAGGCCA
CCCTGGCGCACTTCCGCTAA

Downstream 100 bases:

>100_bases
AAGGGTCCTGCCGTAAAGCCGATGCCGAGCTCGTTGTCGCAACGTCAAAACCTGATGAAATTTTTGGGCTGGATGCTTTG
CCTGCTGATCGCTATCCCTG

Product: bifunctional dihydrofolate/folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MSKPTSPSKEDLSDLIPRFDQRGMDLDLERMQQALQAMGNPCASIPAIQVVGTNGKGSIASFIASSLKAAGIRVGLTTSP
HLVSWCERISSDGEPISIVELRQRLTALQPLAQTHRLTPFELLIATAFDHFRSREVELLVLEVGLGGRLDATTAHPYRPI
IAMANIGLDHCEHLGYSLKEITAEKSAVISPGAAVISARQQTEVASILEDKAKHQQARLQWVSPLPENWDLGLPGVLQRQ
NAAVAKGALESLAPLGWRLNQDVIRTGLAHAYWPARLQTVHWQSQPVLIDGAHNPPAAERLAHERQHWSNQEFGVCWVLG
LQAHKQAPAMLRHLLKPSDLAWIVPVPEHCSWTQHQLAAHCPDLSSQLQSADNVEQVFSILLKQNRWPNPPPVVAGSLYL
LGDLLAKQTIQAE

Sequences:

>Translated_413_residues
MSKPTSPSKEDLSDLIPRFDQRGMDLDLERMQQALQAMGNPCASIPAIQVVGTNGKGSIASFIASSLKAAGIRVGLTTSP
HLVSWCERISSDGEPISIVELRQRLTALQPLAQTHRLTPFELLIATAFDHFRSREVELLVLEVGLGGRLDATTAHPYRPI
IAMANIGLDHCEHLGYSLKEITAEKSAVISPGAAVISARQQTEVASILEDKAKHQQARLQWVSPLPENWDLGLPGVLQRQ
NAAVAKGALESLAPLGWRLNQDVIRTGLAHAYWPARLQTVHWQSQPVLIDGAHNPPAAERLAHERQHWSNQEFGVCWVLG
LQAHKQAPAMLRHLLKPSDLAWIVPVPEHCSWTQHQLAAHCPDLSSQLQSADNVEQVFSILLKQNRWPNPPPVVAGSLYL
LGDLLAKQTIQAE
>Mature_412_residues
SKPTSPSKEDLSDLIPRFDQRGMDLDLERMQQALQAMGNPCASIPAIQVVGTNGKGSIASFIASSLKAAGIRVGLTTSPH
LVSWCERISSDGEPISIVELRQRLTALQPLAQTHRLTPFELLIATAFDHFRSREVELLVLEVGLGGRLDATTAHPYRPII
AMANIGLDHCEHLGYSLKEITAEKSAVISPGAAVISARQQTEVASILEDKAKHQQARLQWVSPLPENWDLGLPGVLQRQN
AAVAKGALESLAPLGWRLNQDVIRTGLAHAYWPARLQTVHWQSQPVLIDGAHNPPAAERLAHERQHWSNQEFGVCWVLGL
QAHKQAPAMLRHLLKPSDLAWIVPVPEHCSWTQHQLAAHCPDLSSQLQSADNVEQVFSILLKQNRWPNPPPVVAGSLYLL
GDLLAKQTIQAE

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932990, Length=380, Percent_Identity=27.8947368421053, Blast_Score=112, Evalue=9e-25,
Organism=Homo sapiens, GI66932984, Length=380, Percent_Identity=27.8947368421053, Blast_Score=111, Evalue=9e-25,
Organism=Escherichia coli, GI1788654, Length=401, Percent_Identity=28.6783042394015, Blast_Score=112, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI17553148, Length=331, Percent_Identity=27.190332326284, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17553150, Length=331, Percent_Identity=27.190332326284, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI71984923, Length=331, Percent_Identity=27.190332326284, Blast_Score=102, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6323760, Length=418, Percent_Identity=30.622009569378, Blast_Score=169, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6324815, Length=281, Percent_Identity=29.5373665480427, Blast_Score=104, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24581568, Length=181, Percent_Identity=33.7016574585635, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24641571, Length=274, Percent_Identity=28.1021897810219, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 45351; Mature: 45220

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPTSPSKEDLSDLIPRFDQRGMDLDLERMQQALQAMGNPCASIPAIQVVGTNGKGSIA
CCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHH
SFIASSLKAAGIRVGLTTSPHLVSWCERISSDGEPISIVELRQRLTALQPLAQTHRLTPF
HHHHHHHHHCCEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHH
ELLIATAFDHFRSREVELLVLEVGLGGRLDATTAHPYRPIIAMANIGLDHCEHLGYSLKE
HHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHCCHHHHHHCCCHHHH
ITAEKSAVISPGAAVISARQQTEVASILEDKAKHQQARLQWVSPLPENWDLGLPGVLQRQ
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHC
NAAVAKGALESLAPLGWRLNQDVIRTGLAHAYWPARLQTVHWQSQPVLIDGAHNPPAAER
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHEEEEECCCCEEEECCCCCCHHHH
LAHERQHWSNQEFGVCWVLGLQAHKQAPAMLRHLLKPSDLAWIVPVPEHCSWTQHQLAAH
HHHHHHHCCCCCCCEEEEEECHHHHCHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHH
CPDLSSQLQSADNVEQVFSILLKQNRWPNPPPVVAGSLYLLGDLLAKQTIQAE
CCCHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKPTSPSKEDLSDLIPRFDQRGMDLDLERMQQALQAMGNPCASIPAIQVVGTNGKGSIA
CCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHH
SFIASSLKAAGIRVGLTTSPHLVSWCERISSDGEPISIVELRQRLTALQPLAQTHRLTPF
HHHHHHHHHCCEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHH
ELLIATAFDHFRSREVELLVLEVGLGGRLDATTAHPYRPIIAMANIGLDHCEHLGYSLKE
HHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHCCHHHHHHCCCHHHH
ITAEKSAVISPGAAVISARQQTEVASILEDKAKHQQARLQWVSPLPENWDLGLPGVLQRQ
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHC
NAAVAKGALESLAPLGWRLNQDVIRTGLAHAYWPARLQTVHWQSQPVLIDGAHNPPAAER
CHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHEEEEECCCCEEEECCCCCCHHHH
LAHERQHWSNQEFGVCWVLGLQAHKQAPAMLRHLLKPSDLAWIVPVPEHCSWTQHQLAAH
HHHHHHHCCCCCCCEEEEEECHHHHCHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHH
CPDLSSQLQSADNVEQVFSILLKQNRWPNPPPVVAGSLYLLGDLLAKQTIQAE
CCCHHHHHHCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]