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The map label for this gene is cysK1 [H]

Identifier: 33862497

GI number: 33862497

Start: 258765

End: 259751

Strand: Reverse

Name: cysK1 [H]

Synonym: PMT0224

Alternate gene names: 33862497

Gene position: 259751-258765 (Counterclockwise)

Preceding gene: 33862498

Following gene: 33862494

Centisome position: 10.77

GC content: 54.81

Gene sequence:

>987_bases
ATGTCCCGCATTTACGAAGACAACAGCCAGGCAATCGGCAACACTCCACTGGTCAAACTGCACGCCGTCAGCAAAAACGC
CAAGGCAACTGTTCTCGCAAAGATTGAAGGCCGCAATCCCGCCTACAGCGTGAAATGCCGCATTGGCGCCAACATGATCT
GGGATGCCGAAAAGCGCGGTTTACTGAGCAAAGAGCGCACGATCATTGAGCCCACCTCTGGTAACACCGGCATTGCACTG
GCCTACACAGCAGCAGCCCGCGGCTACAAACTGATCCTCACCATGCCCGAATCAATGTCGCTAGAACGGCGGCGGGTGAT
GGCAGTGCTTGGTGCAGAACTCATTCTCACAGAAGCAGCCAAGGGAATGCCAGGCGCCATTGCCAAGGCAAAAGAGATCG
CCGAAAGCGATCCCCAGAAATACTTCATGCCCGGTCAGTTTGAGAATCCGGCCAATCCCGACATCCACAGCAAAACAACC
GGTCCAGAAATCTGGAACGACTGTGATGGCGCCATTGATGTGCTCGTGTCTGGCGTAGGCACCGGCGGCACGATCACAGG
TGTCTCCCGTTACATCAAGCAGGAGAAAGGCAAAGCCATCATTTCTGTAGCCGTCGAACCAACCCATAGCCCGGTTATCA
GCCAAACCCTCAACGGGGAAGAGGTCAAACCTGGTCCTCACAAAATCCAGGGCATTGGAGCCGGCTTCATTCCCAAAAAC
CTCGATCTTTCTTTAGTGGATCGTGTGGAGCAGGTCAGCAATGATGAATCCATCGCCATGGCCCTGCGCTTGGCAAATGA
AGAAGGTCTACTGGTGGGCATCTCCAGTGGTGCGGCAGTTGCAGCGGCTATTCGTCTGGCAGAACAACCCGAATTTGCGG
GCAAAACCATCGTGGTCGTGCTGCCAGACATGGCCGAGCGTTATCTCTCTTCAGTGATGTTTGAGAGTGTGCCCACAGGC
ATCATTCAGGCCCCTGTAGCTGCCTAA

Upstream 100 bases:

>100_bases
CTAACCGTAAGCAACCCATCACATGGTCGTCACAACAGATCATCGTCCTCACCAATAGAGCCAAATTCCAGCTAATGATG
TGGTCGATTCATATGCACGC

Downstream 100 bases:

>100_bases
ATCGCCTCCAGCTAGAAACTGATAACTGATCAACAGGGGCGGGCCGCTGGCAACACCGCCTCTGGATCAATCCACATCGC
ATCGCCATAAGAGAAAAAGC

Product: O-acetylserine (thiol)-lyase A

Products: NA

Alternate protein names: CSase; O-acetylserine (thiol)-lyase; OAS-TL; O-acetylserine sulfhydrylase [H]

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MSRIYEDNSQAIGNTPLVKLHAVSKNAKATVLAKIEGRNPAYSVKCRIGANMIWDAEKRGLLSKERTIIEPTSGNTGIAL
AYTAAARGYKLILTMPESMSLERRRVMAVLGAELILTEAAKGMPGAIAKAKEIAESDPQKYFMPGQFENPANPDIHSKTT
GPEIWNDCDGAIDVLVSGVGTGGTITGVSRYIKQEKGKAIISVAVEPTHSPVISQTLNGEEVKPGPHKIQGIGAGFIPKN
LDLSLVDRVEQVSNDESIAMALRLANEEGLLVGISSGAAVAAAIRLAEQPEFAGKTIVVVLPDMAERYLSSVMFESVPTG
IIQAPVAA

Sequences:

>Translated_328_residues
MSRIYEDNSQAIGNTPLVKLHAVSKNAKATVLAKIEGRNPAYSVKCRIGANMIWDAEKRGLLSKERTIIEPTSGNTGIAL
AYTAAARGYKLILTMPESMSLERRRVMAVLGAELILTEAAKGMPGAIAKAKEIAESDPQKYFMPGQFENPANPDIHSKTT
GPEIWNDCDGAIDVLVSGVGTGGTITGVSRYIKQEKGKAIISVAVEPTHSPVISQTLNGEEVKPGPHKIQGIGAGFIPKN
LDLSLVDRVEQVSNDESIAMALRLANEEGLLVGISSGAAVAAAIRLAEQPEFAGKTIVVVLPDMAERYLSSVMFESVPTG
IIQAPVAA
>Mature_327_residues
SRIYEDNSQAIGNTPLVKLHAVSKNAKATVLAKIEGRNPAYSVKCRIGANMIWDAEKRGLLSKERTIIEPTSGNTGIALA
YTAAARGYKLILTMPESMSLERRRVMAVLGAELILTEAAKGMPGAIAKAKEIAESDPQKYFMPGQFENPANPDIHSKTTG
PEIWNDCDGAIDVLVSGVGTGGTITGVSRYIKQEKGKAIISVAVEPTHSPVISQTLNGEEVKPGPHKIQGIGAGFIPKNL
DLSLVDRVEQVSNDESIAMALRLANEEGLLVGISSGAAVAAAIRLAEQPEFAGKTIVVVLPDMAERYLSSVMFESVPTGI
IQAPVAA

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=316, Percent_Identity=39.2405063291139, Blast_Score=202, Evalue=3e-52,
Organism=Homo sapiens, GI295821200, Length=316, Percent_Identity=39.2405063291139, Blast_Score=202, Evalue=3e-52,
Organism=Homo sapiens, GI4557415, Length=316, Percent_Identity=39.2405063291139, Blast_Score=202, Evalue=3e-52,
Organism=Escherichia coli, GI1788754, Length=320, Percent_Identity=65.9375, Blast_Score=412, Evalue=1e-116,
Organism=Escherichia coli, GI2367138, Length=305, Percent_Identity=42.9508196721311, Blast_Score=208, Evalue=5e-55,
Organism=Caenorhabditis elegans, GI17535051, Length=317, Percent_Identity=50.4731861198738, Blast_Score=292, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI115535073, Length=316, Percent_Identity=51.8987341772152, Blast_Score=272, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI17562970, Length=326, Percent_Identity=45.398773006135, Blast_Score=271, Evalue=5e-73,
Organism=Caenorhabditis elegans, GI32566674, Length=312, Percent_Identity=47.1153846153846, Blast_Score=268, Evalue=4e-72,
Organism=Caenorhabditis elegans, GI17561720, Length=330, Percent_Identity=44.8484848484849, Blast_Score=244, Evalue=6e-65,
Organism=Caenorhabditis elegans, GI32566672, Length=229, Percent_Identity=44.5414847161572, Blast_Score=195, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17534315, Length=323, Percent_Identity=41.1764705882353, Blast_Score=160, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI25147552, Length=313, Percent_Identity=38.6581469648562, Blast_Score=145, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17561716, Length=138, Percent_Identity=42.0289855072464, Blast_Score=99, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6321594, Length=315, Percent_Identity=37.1428571428571, Blast_Score=176, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6321449, Length=330, Percent_Identity=33.030303030303, Blast_Score=127, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24643623, Length=313, Percent_Identity=39.6166134185303, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI20129101, Length=313, Percent_Identity=39.6166134185303, Blast_Score=169, Evalue=3e-42,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 34756; Mature: 34625

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIYEDNSQAIGNTPLVKLHAVSKNAKATVLAKIEGRNPAYSVKCRIGANMIWDAEKRG
CCCEECCCCCCCCCCCEEEEEEECCCCCEEEEEEEECCCCCEEEEEEECCCEEECCHHCC
LLSKERTIIEPTSGNTGIALAYTAAARGYKLILTMPESMSLERRRVMAVLGAELILTEAA
CCCCCCEEEECCCCCCCEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHC
KGMPGAIAKAKEIAESDPQKYFMPGQFENPANPDIHSKTTGPEIWNDCDGAIDVLVSGVG
CCCCCHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHCCC
TGGTITGVSRYIKQEKGKAIISVAVEPTHSPVISQTLNGEEVKPGPHKIQGIGAGFIPKN
CCCHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHCCCCCCCCCCCCEEEECCCCCCCCC
LDLSLVDRVEQVSNDESIAMALRLANEEGLLVGISSGAAVAAAIRLAEQPEFAGKTIVVV
CCHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEECCCHHHHHHHHHHCCCCCCCCEEEEE
LPDMAERYLSSVMFESVPTGIIQAPVAA
CCHHHHHHHHHHHHHHCCCCHHCCCCCC
>Mature Secondary Structure 
SRIYEDNSQAIGNTPLVKLHAVSKNAKATVLAKIEGRNPAYSVKCRIGANMIWDAEKRG
CCEECCCCCCCCCCCEEEEEEECCCCCEEEEEEEECCCCCEEEEEEECCCEEECCHHCC
LLSKERTIIEPTSGNTGIALAYTAAARGYKLILTMPESMSLERRRVMAVLGAELILTEAA
CCCCCCEEEECCCCCCCEEEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHC
KGMPGAIAKAKEIAESDPQKYFMPGQFENPANPDIHSKTTGPEIWNDCDGAIDVLVSGVG
CCCCCHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCHHHCCCCHHHHHHHHCCC
TGGTITGVSRYIKQEKGKAIISVAVEPTHSPVISQTLNGEEVKPGPHKIQGIGAGFIPKN
CCCHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHCCCCCCCCCCCCEEEECCCCCCCCC
LDLSLVDRVEQVSNDESIAMALRLANEEGLLVGISSGAAVAAAIRLAEQPEFAGKTIVVV
CCHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEECCCHHHHHHHHHHCCCCCCCCEEEEE
LPDMAERYLSSVMFESVPTGIIQAPVAA
CCHHHHHHHHHHHHHHCCCCHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7603442 [H]