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The map label for this gene is mccB [H]

Identifier: 33862498

GI number: 33862498

Start: 259848

End: 261320

Strand: Reverse

Name: mccB [H]

Synonym: PMT0225

Alternate gene names: 33862498

Gene position: 261320-259848 (Counterclockwise)

Preceding gene: 33862499

Following gene: 33862497

Centisome position: 10.84

GC content: 59.27

Gene sequence:

>1473_bases
GTGAGCCCACGCGACCTACTGCAGGATCCCTGCTGGCACGCTGATGAGCTCGGCAAAGCTCTACCAGATAGTCCCCATGC
CGTTTCGGTAGCCCTACCCCGCTGGCAAGACGTGGTCGCCTATGAGGAGAAAGACCCTGCTTGTCTTAAAGCCCTGCAGG
CCATCTACCCCCGTTTCGGCTTCCATCCACTGGTAGCCGAGATCGCCCAAAAAGCACGAAAAGAGCATGGCTGTGCAGAT
GATGGCAGTGCCTGGCCCTATCCCACTGCTGCTGCCGCTGAGCGAGCCAGATTTCATTGCCAACGCAGTGAGCCGATGGC
AAAAACAAGCATCAAAACCGTACTGGGCCTGCCATGTTTGCTCACCGACGCAAAAGGCTGCAGCGCTGCCAAGGCCTTCT
GGCAACACAGCGGCCTTGGTGCATCCTCACGTCAGGCTGCTAGCGCACTTGGGCAGGAACCATTCCCCTCACTGCAAGCC
AGTGCAGCCGCCCAGACGTTGCTAAAACAACGACTCGCAGAGATCTATGGCTGCGACCCAGAGCTCGTGCAGCTGCATCC
CTCCGGGATGGCTGCTCTCACCAAAGCCCTGCAAGCGATTACGGCTCTACGCCCCGGCAAACCCTGCTTGCAGCTTGGCT
TCCCCTATGTGGATGTGCTCAAGCTGCCTGAGGTGGTGTTTGGAGGTTGCGACCTTCTACTGACAACAGATCTGAACCGA
TTGGCCCTGGAACTTGATCGCAGACAACCAGCCGCTGTGATCGTTGAGCTCCCTAGCAACCCCATGCTGCAGTGCGTCGA
TCTGCCAGCGGTAGCCCGCCTAGCAAAGGCTCGAGGTATCCCCGTGATCGCTGATGACACCATCGGCTCACCACTCAATC
TTGATGCGCTGCCCCATGCAGATCTGGTGTTTAGCTCCCTCACCAAAAGCTTCGCCGGCCGTGGTGACATCCTGGCGGGT
TCTCTCGTCGTCAGCCCTAGCTCCCCCTGGCGAGAGGAACTCTCCAGAGAGCTCTCGACTGCCCTTGCTCCCCTGGGGGA
TGCTGATGCCATCGCCTTAGAACAAGCCAGCCGAGATGTGGCCCAGCGGCTGCCGCAGCTCAACAACGCCTGCCAAAGAT
TGGCAGACAAGCTGGCCTGTCATCCCTCTATTGCTCGAGTACACCACCCAGGCCAATGCACCAACTTCCAAGCCCTGATG
CGCCCTGGCGCCGGCCACGGCTGCCTGCTCTCGTTTGAGCTATGGGGGGGGATAGAGCAAGCCAAGCGGGTGTATGACGC
CCTGCAGGTCTGCAAAGGTCCAAGCCTCGGCACAAGCTTCACGCTGGTGTGCCCCTATGTACTGCTCGCCCATTACAACG
AACTGAGCTGGGCAGAATCCTGTGGAGTACCCAGCCATCTGCTGCGCGTATCCGTAGGGCTTGAAGATCCCGATAATCTT
TGGCGCCGCTTTGAGATGGCGTTGAAGGCCTAA

Upstream 100 bases:

>100_bases
GAAAACTTGGCATCGATGATGGTCTGGTGCGCTTCTCCGTGGGCTGCGAAGACCTCGCTGATCTCAGCAGAGATTTAGAT
CAAGCCCTCAAACAGCTGTA

Downstream 100 bases:

>100_bases
CCGTAAGCAACCCATCACATGGTCGTCACAACAGATCATCGTCCTCACCAATAGAGCCAAATTCCAGCTAATGATGTGGT
CGATTCATATGCACGCATGT

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: Gamma-cystathionase; Homocysteine gamma-lyase [H]

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MSPRDLLQDPCWHADELGKALPDSPHAVSVALPRWQDVVAYEEKDPACLKALQAIYPRFGFHPLVAEIAQKARKEHGCAD
DGSAWPYPTAAAAERARFHCQRSEPMAKTSIKTVLGLPCLLTDAKGCSAAKAFWQHSGLGASSRQAASALGQEPFPSLQA
SAAAQTLLKQRLAEIYGCDPELVQLHPSGMAALTKALQAITALRPGKPCLQLGFPYVDVLKLPEVVFGGCDLLLTTDLNR
LALELDRRQPAAVIVELPSNPMLQCVDLPAVARLAKARGIPVIADDTIGSPLNLDALPHADLVFSSLTKSFAGRGDILAG
SLVVSPSSPWREELSRELSTALAPLGDADAIALEQASRDVAQRLPQLNNACQRLADKLACHPSIARVHHPGQCTNFQALM
RPGAGHGCLLSFELWGGIEQAKRVYDALQVCKGPSLGTSFTLVCPYVLLAHYNELSWAESCGVPSHLLRVSVGLEDPDNL
WRRFEMALKA

Sequences:

>Translated_490_residues
MSPRDLLQDPCWHADELGKALPDSPHAVSVALPRWQDVVAYEEKDPACLKALQAIYPRFGFHPLVAEIAQKARKEHGCAD
DGSAWPYPTAAAAERARFHCQRSEPMAKTSIKTVLGLPCLLTDAKGCSAAKAFWQHSGLGASSRQAASALGQEPFPSLQA
SAAAQTLLKQRLAEIYGCDPELVQLHPSGMAALTKALQAITALRPGKPCLQLGFPYVDVLKLPEVVFGGCDLLLTTDLNR
LALELDRRQPAAVIVELPSNPMLQCVDLPAVARLAKARGIPVIADDTIGSPLNLDALPHADLVFSSLTKSFAGRGDILAG
SLVVSPSSPWREELSRELSTALAPLGDADAIALEQASRDVAQRLPQLNNACQRLADKLACHPSIARVHHPGQCTNFQALM
RPGAGHGCLLSFELWGGIEQAKRVYDALQVCKGPSLGTSFTLVCPYVLLAHYNELSWAESCGVPSHLLRVSVGLEDPDNL
WRRFEMALKA
>Mature_489_residues
SPRDLLQDPCWHADELGKALPDSPHAVSVALPRWQDVVAYEEKDPACLKALQAIYPRFGFHPLVAEIAQKARKEHGCADD
GSAWPYPTAAAAERARFHCQRSEPMAKTSIKTVLGLPCLLTDAKGCSAAKAFWQHSGLGASSRQAASALGQEPFPSLQAS
AAAQTLLKQRLAEIYGCDPELVQLHPSGMAALTKALQAITALRPGKPCLQLGFPYVDVLKLPEVVFGGCDLLLTTDLNRL
ALELDRRQPAAVIVELPSNPMLQCVDLPAVARLAKARGIPVIADDTIGSPLNLDALPHADLVFSSLTKSFAGRGDILAGS
LVVSPSSPWREELSRELSTALAPLGDADAIALEQASRDVAQRLPQLNNACQRLADKLACHPSIARVHHPGQCTNFQALMR
PGAGHGCLLSFELWGGIEQAKRVYDALQVCKGPSLGTSFTLVCPYVLLAHYNELSWAESCGVPSHLLRVSVGLEDPDNLW
RRFEMALKA

Specific function: Catalyzes the conversion of cystathionine to cysteine, and homocysteine to sulfide [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1790375, Length=247, Percent_Identity=29.1497975708502, Blast_Score=89, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI71980708, Length=251, Percent_Identity=26.2948207171315, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6322970, Length=568, Percent_Identity=29.5774647887324, Blast_Score=195, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6322590, Length=439, Percent_Identity=31.4350797266515, Blast_Score=186, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6323557, Length=352, Percent_Identity=34.375, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6319307, Length=278, Percent_Identity=28.0575539568345, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19922554, Length=297, Percent_Identity=23.9057239057239, Blast_Score=76, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.1; =4.4.1.2 [H]

Molecular weight: Translated: 52729; Mature: 52598

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPRDLLQDPCWHADELGKALPDSPHAVSVALPRWQDVVAYEEKDPACLKALQAIYPRFG
CCCHHHHHCCCCCHHHHHHHCCCCCCEEEEECCCHHHHHHCCCCCHHHHHHHHHHHHCCC
FHPLVAEIAQKARKEHGCADDGSAWPYPTAAAAERARFHCQRSEPMAKTSIKTVLGLPCL
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHH
LTDAKGCSAAKAFWQHSGLGASSRQAASALGQEPFPSLQASAAAQTLLKQRLAEIYGCDP
EECCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCH
ELVQLHPSGMAALTKALQAITALRPGKPCLQLGFPYVDVLKLPEVVFGGCDLLLTTDLNR
HHEEECCCCHHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHCCCCEEEECCHHH
LALELDRRQPAAVIVELPSNPMLQCVDLPAVARLAKARGIPVIADDTIGSPLNLDALPHA
HHHHHHCCCCCEEEEECCCCCCEEEECCHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCH
DLVFSSLTKSFAGRGDILAGSLVVSPSSPWREELSRELSTALAPLGDADAIALEQASRDV
HHHHHHHHHHHCCCCCEEECEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
AQRLPQLNNACQRLADKLACHPSIARVHHPGQCTNFQALMRPGAGHGCLLSFELWGGIEQ
HHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCHHHHHCCCCCCCEEEEEHHHCCHHH
AKRVYDALQVCKGPSLGTSFTLVCPYVLLAHYNELSWAESCGVPSHLLRVSVGLEDPDNL
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHEEECCCCCHHHH
WRRFEMALKA
HHHHHHHHCC
>Mature Secondary Structure 
SPRDLLQDPCWHADELGKALPDSPHAVSVALPRWQDVVAYEEKDPACLKALQAIYPRFG
CCHHHHHCCCCCHHHHHHHCCCCCCEEEEECCCHHHHHHCCCCCHHHHHHHHHHHHCCC
FHPLVAEIAQKARKEHGCADDGSAWPYPTAAAAERARFHCQRSEPMAKTSIKTVLGLPCL
CCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHH
LTDAKGCSAAKAFWQHSGLGASSRQAASALGQEPFPSLQASAAAQTLLKQRLAEIYGCDP
EECCCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCH
ELVQLHPSGMAALTKALQAITALRPGKPCLQLGFPYVDVLKLPEVVFGGCDLLLTTDLNR
HHEEECCCCHHHHHHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHCCCCEEEECCHHH
LALELDRRQPAAVIVELPSNPMLQCVDLPAVARLAKARGIPVIADDTIGSPLNLDALPHA
HHHHHHCCCCCEEEEECCCCCCEEEECCHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCH
DLVFSSLTKSFAGRGDILAGSLVVSPSSPWREELSRELSTALAPLGDADAIALEQASRDV
HHHHHHHHHHHCCCCCEEECEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
AQRLPQLNNACQRLADKLACHPSIARVHHPGQCTNFQALMRPGAGHGCLLSFELWGGIEQ
HHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCHHHHHCCCCCCCEEEEEHHHCCHHH
AKRVYDALQVCKGPSLGTSFTLVCPYVLLAHYNELSWAESCGVPSHLLRVSVGLEDPDNL
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHEEECCCCCHHHH
WRRFEMALKA
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9308178; 9384377 [H]