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The map label for this gene is yaaJ [C]

Identifier: 33862466

GI number: 33862466

Start: 224171

End: 225565

Strand: Reverse

Name: yaaJ [C]

Synonym: PMT0193

Alternate gene names: 33862466

Gene position: 225565-224171 (Counterclockwise)

Preceding gene: 33862470

Following gene: 33862464

Centisome position: 9.36

GC content: 55.48

Gene sequence:

>1395_bases
ATGGATGTGTTGCCAGCGTCGCTCTTGACGAATTTATCCTTACAACTCGGACAGTTCCCAAGCGGACTGGAAGACGCAGT
CGAGGCCATCAATAATCCGATCAACGGCTTTGCCTGGGGTTGGCCCACAGTCATCCTGATTGCAGGCACCGGCATCTTGC
TGATGGTTGGGCTGGGCTTTATGCCCTTACTGCGCATCCCCTATGGAGTGCGCATGCTGCTTCGCAATCCAACCTCCTCT
AGCGAGGGCGAAATCAGTCCATTCCAGGCACTGATGACCTCGATGGCGGCCACGATCGGCACGGGCAACATCGCCGGTGT
CGCTGTTGCGATCGCCATGGGCGGCCCTGGGGCGGTGTTCTGGATGTGGCTAATTGCCATTTTTGGTATTGCCACCAAGT
ACGCCGAAGCCTTACTTGCAGTTCACTTCCGCGAAGTGGACCCCCTCGGCAATCATGTCGGTGGTCCGATGTACTACATC
CGCAATGGCCTAGGTCCAAACTGGGCCTGGCTGGGCGGATTCTTTGCCCTGTTTGGAATGCTGGCGGGCTTTGGCATTGG
CAATGGCGTGCAATCGTTTGAGGTCTCCAGTGCCTTAGCCACGATCGGCATCCCTCGGCTTTTAACGGGTGTCGTGCTTG
GAGTGCTTGTCTTTGGAGTCATCATTGGCGGCATCAAACGCATCGCCCAGGCTGCATCCGCCATCGTTCCTTTGATGTCG
TTGTTTTATGTGATTGCTTGCCTGGTCATCATTCTCAGCAACATCAGCGAAGTGCCAGAAGCGTTCTCAACGATCTTCTC
CAATGCCTTCACAGGCGAAGCCGCTGCCAGCGGCACGTTGACCCAAGTGATCCTGATGGGCTTCAAGCGCGGCATCTTCT
CCAATGAAGCTGGTCTTGGTAGTGCGCCTATCGCTCACGCTGCCGCCAACACCAATGACCCAGTCCGTCAGGGCACTATC
GCCATGCTTGGAACCTTCATCGATACTTTGGTCATCTGCACAATGACGGCTCTGGTGATCATCACCACCGGCGCCTATCA
GAGTGGTGAGTCAGGCTCTGATCTATCAATCGCTGCCTTCAACAGTGGCCTTGCAGGCTCAGGTTGGGTCGTGACAGCTG
GCCTCGTGGTGTTTGCCCTAACAACAGTTCTTGGCTGGGGCTTTTACAGCGAACGCTGCACTGAATATCTCTTTGGGGTG
CAAGCCATTCTTCCCTTCCGCCTGGTGTGGGTCGCTGTGGTTGTCATTGGTGCTGTTGCAGGCAATCGCGGTGTGGTGTG
GGACGTAGCTGACACACTTAATGGTCTGATGGCGATTCCTAACTTGATCGCACTGGTGCTGCTCTCAGGCACTGTCTTCC
GCCTCTCCAAAAACTACCAATTTGAAGAGGGCTAA

Upstream 100 bases:

>100_bases
AACGCTTTCTACTGCTGATGTAACAACCTCAAAGGCAGCCTGATACGAACATTGCCGTCAGCAGGAGCATCTGACAACAT
CTTTGAACGCCTTTGCAAAA

Downstream 100 bases:

>100_bases
ACGTAGAGGTGATCTTGATCAGTGAAACAACACTGCTGATTGAGGGCCTAATCCCCGGCCATTGCATTCCAATGCTCCTT
CCAGCCAAAGAGGGTCTGTC

Product: sodium/alanine symporter family protein

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MDVLPASLLTNLSLQLGQFPSGLEDAVEAINNPINGFAWGWPTVILIAGTGILLMVGLGFMPLLRIPYGVRMLLRNPTSS
SEGEISPFQALMTSMAATIGTGNIAGVAVAIAMGGPGAVFWMWLIAIFGIATKYAEALLAVHFREVDPLGNHVGGPMYYI
RNGLGPNWAWLGGFFALFGMLAGFGIGNGVQSFEVSSALATIGIPRLLTGVVLGVLVFGVIIGGIKRIAQAASAIVPLMS
LFYVIACLVIILSNISEVPEAFSTIFSNAFTGEAAASGTLTQVILMGFKRGIFSNEAGLGSAPIAHAAANTNDPVRQGTI
AMLGTFIDTLVICTMTALVIITTGAYQSGESGSDLSIAAFNSGLAGSGWVVTAGLVVFALTTVLGWGFYSERCTEYLFGV
QAILPFRLVWVAVVVIGAVAGNRGVVWDVADTLNGLMAIPNLIALVLLSGTVFRLSKNYQFEEG

Sequences:

>Translated_464_residues
MDVLPASLLTNLSLQLGQFPSGLEDAVEAINNPINGFAWGWPTVILIAGTGILLMVGLGFMPLLRIPYGVRMLLRNPTSS
SEGEISPFQALMTSMAATIGTGNIAGVAVAIAMGGPGAVFWMWLIAIFGIATKYAEALLAVHFREVDPLGNHVGGPMYYI
RNGLGPNWAWLGGFFALFGMLAGFGIGNGVQSFEVSSALATIGIPRLLTGVVLGVLVFGVIIGGIKRIAQAASAIVPLMS
LFYVIACLVIILSNISEVPEAFSTIFSNAFTGEAAASGTLTQVILMGFKRGIFSNEAGLGSAPIAHAAANTNDPVRQGTI
AMLGTFIDTLVICTMTALVIITTGAYQSGESGSDLSIAAFNSGLAGSGWVVTAGLVVFALTTVLGWGFYSERCTEYLFGV
QAILPFRLVWVAVVVIGAVAGNRGVVWDVADTLNGLMAIPNLIALVLLSGTVFRLSKNYQFEEG
>Mature_464_residues
MDVLPASLLTNLSLQLGQFPSGLEDAVEAINNPINGFAWGWPTVILIAGTGILLMVGLGFMPLLRIPYGVRMLLRNPTSS
SEGEISPFQALMTSMAATIGTGNIAGVAVAIAMGGPGAVFWMWLIAIFGIATKYAEALLAVHFREVDPLGNHVGGPMYYI
RNGLGPNWAWLGGFFALFGMLAGFGIGNGVQSFEVSSALATIGIPRLLTGVVLGVLVFGVIIGGIKRIAQAASAIVPLMS
LFYVIACLVIILSNISEVPEAFSTIFSNAFTGEAAASGTLTQVILMGFKRGIFSNEAGLGSAPIAHAAANTNDPVRQGTI
AMLGTFIDTLVICTMTALVIITTGAYQSGESGSDLSIAAFNSGLAGSGWVVTAGLVVFALTTVLGWGFYSERCTEYLFGV
QAILPFRLVWVAVVVIGAVAGNRGVVWDVADTLNGLMAIPNLIALVLLSGTVFRLSKNYQFEEG

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=442, Percent_Identity=38.4615384615385, Blast_Score=223, Evalue=2e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 48410; Mature: 48410

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00873 NA_ALANINE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVLPASLLTNLSLQLGQFPSGLEDAVEAINNPINGFAWGWPTVILIAGTGILLMVGLGF
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHCHHHHHHHHHHH
MPLLRIPYGVRMLLRNPTSSSEGEISPFQALMTSMAATIGTGNIAGVAVAIAMGGPGAVF
HHHHHCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHEEEEECCCHHHH
WMWLIAIFGIATKYAEALLAVHFREVDPLGNHVGGPMYYIRNGLGPNWAWLGGFFALFGM
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHH
LAGFGIGNGVQSFEVSSALATIGIPRLLTGVVLGVLVFGVIIGGIKRIAQAASAIVPLMS
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFYVIACLVIILSNISEVPEAFSTIFSNAFTGEAAASGTLTQVILMGFKRGIFSNEAGLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHHCCCCCCCCCC
SAPIAHAAANTNDPVRQGTIAMLGTFIDTLVICTMTALVIITTGAYQSGESGSDLSIAAF
CCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEE
NSGLAGSGWVVTAGLVVFALTTVLGWGFYSERCTEYLFGVQAILPFRLVWVAVVVIGAVA
CCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GNRGVVWDVADTLNGLMAIPNLIALVLLSGTVFRLSKNYQFEEG
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHCHHEEECCCCCCCCC
>Mature Secondary Structure
MDVLPASLLTNLSLQLGQFPSGLEDAVEAINNPINGFAWGWPTVILIAGTGILLMVGLGF
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHCHHHHHHHHHHH
MPLLRIPYGVRMLLRNPTSSSEGEISPFQALMTSMAATIGTGNIAGVAVAIAMGGPGAVF
HHHHHCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHEEEEECCCHHHH
WMWLIAIFGIATKYAEALLAVHFREVDPLGNHVGGPMYYIRNGLGPNWAWLGGFFALFGM
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHH
LAGFGIGNGVQSFEVSSALATIGIPRLLTGVVLGVLVFGVIIGGIKRIAQAASAIVPLMS
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFYVIACLVIILSNISEVPEAFSTIFSNAFTGEAAASGTLTQVILMGFKRGIFSNEAGLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHHCCCCCCCCCC
SAPIAHAAANTNDPVRQGTIAMLGTFIDTLVICTMTALVIITTGAYQSGESGSDLSIAAF
CCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEE
NSGLAGSGWVVTAGLVVFALTTVLGWGFYSERCTEYLFGVQAILPFRLVWVAVVVIGAVA
CCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GNRGVVWDVADTLNGLMAIPNLIALVLLSGTVFRLSKNYQFEEG
CCCCEEEEHHHHHHHHHHHHHHHHHHHHHCHHEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]