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The map label for this gene is mtnD [H]

Identifier: 33862470

GI number: 33862470

Start: 229093

End: 229650

Strand: Reverse

Name: mtnD [H]

Synonym: PMT0197

Alternate gene names: 33862470

Gene position: 229650-229093 (Counterclockwise)

Preceding gene: 33862473

Following gene: 33862466

Centisome position: 9.53

GC content: 56.09

Gene sequence:

>558_bases
ATGAGTCGCCTCAGCATCCACCCCGAAGGCAGCACCAACGCCACAAGCCCGGCGGAACCCTTATTGGAGAGCGATGATCC
TGCAGTGATCAAAGTCGAGCTGGCCAAAAGGGGCATTGCATTCCAGCGTTGGCCAGCCAAAGTCAAGCTCGATCAGAACT
CAAGCGAGTCAGACATCCTTGCGGCCTACGCCGTCGAGATCGCCCGAGTGCAGGCAGATGGCCGTTACCCCACGGTGGAT
GCAATCCGAATCACGCCAGACCACCCCGATCGTGAAGCGCTGCGCCAAAAATTCTTAGACGAACACACCCATGCCGAAGA
CGAAGTTCGCTTCTTTGTTGAAGGGTGTGGGTTGTTCTGCTTACACATCGGCGCGGAAGTGCTGCAAGTCCTCTGCGAAC
AAAACGACTGCATCAACGTGCCTGCCGGAACTCGCCATTGGTTCGATATGGGCAGCAAGCCACAGTTTTGTGCTGTGCGC
TTCTTCGATAACCCAGAAGGCTGGATTGCAAACTTCACAGGGGATGCGATCGCCGAGAGATTCGCAAAGCTGCCATGA

Upstream 100 bases:

>100_bases
GTTGAAACAGCAGGGCTCGACAAAGCCGAACGAAACATTCAGCCGTCGTTCTAAGACGCCACGATCAAGATGGCTGCAAA
GACCCCTTCAACCTCTGGGA

Downstream 100 bases:

>100_bases
GGCTGAAGTTGGCTTCAGCTATGCATGCGCGGCTGAGGATGCAAGTTGGCGAGGATCCCTGCCTCCACCTCTGCTAACTC
CTGCAAACGAGCAATGATCA

Product: methionine salvage pathway protein E-2/E-2'

Products: NA

Alternate protein names: 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase; DHK-MTPene dioxygenase; Acireductone dioxygenase (Fe(2+)-requiring); ARD'; Fe-ARD; Acireductone dioxygenase (Ni(2+)-requiring); ARD; Ni-ARD [H]

Number of amino acids: Translated: 185; Mature: 184

Protein sequence:

>185_residues
MSRLSIHPEGSTNATSPAEPLLESDDPAVIKVELAKRGIAFQRWPAKVKLDQNSSESDILAAYAVEIARVQADGRYPTVD
AIRITPDHPDREALRQKFLDEHTHAEDEVRFFVEGCGLFCLHIGAEVLQVLCEQNDCINVPAGTRHWFDMGSKPQFCAVR
FFDNPEGWIANFTGDAIAERFAKLP

Sequences:

>Translated_185_residues
MSRLSIHPEGSTNATSPAEPLLESDDPAVIKVELAKRGIAFQRWPAKVKLDQNSSESDILAAYAVEIARVQADGRYPTVD
AIRITPDHPDREALRQKFLDEHTHAEDEVRFFVEGCGLFCLHIGAEVLQVLCEQNDCINVPAGTRHWFDMGSKPQFCAVR
FFDNPEGWIANFTGDAIAERFAKLP
>Mature_184_residues
SRLSIHPEGSTNATSPAEPLLESDDPAVIKVELAKRGIAFQRWPAKVKLDQNSSESDILAAYAVEIARVQADGRYPTVDA
IRITPDHPDREALRQKFLDEHTHAEDEVRFFVEGCGLFCLHIGAEVLQVLCEQNDCINVPAGTRHWFDMGSKPQFCAVRF
FDNPEGWIANFTGDAIAERFAKLP

Specific function: Catalyzes 2 different reactions between oxygene and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4- me

COG id: COG1791

COG function: function code S; Uncharacterized conserved protein, contains double-stranded beta-helix domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acireductone dioxygenase (ARD) family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004313
- InterPro:   IPR011051
- InterPro:   IPR014710 [H]

Pfam domain/function: PF03079 ARD [H]

EC number: =1.13.11.54; =1.13.11.53 [H]

Molecular weight: Translated: 20649; Mature: 20518

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLSIHPEGSTNATSPAEPLLESDDPAVIKVELAKRGIAFQRWPAKVKLDQNSSESDIL
CCCEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEECCCCEEEECCCCCCHHHH
AAYAVEIARVQADGRYPTVDAIRITPDHPDREALRQKFLDEHTHAEDEVRFFVEGCGLFC
HHHHHHHHHEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LHIGAEVLQVLCEQNDCINVPAGTRHWFDMGSKPQFCAVRFFDNPEGWIANFTGDAIAER
HHHHHHHHHHHHCCCCEEECCCCCCHHHCCCCCCCEEEEEEEECCCCEEEECCHHHHHHH
FAKLP
HHCCC
>Mature Secondary Structure 
SRLSIHPEGSTNATSPAEPLLESDDPAVIKVELAKRGIAFQRWPAKVKLDQNSSESDIL
CCEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEECCCCEEEECCCCCCHHHH
AAYAVEIARVQADGRYPTVDAIRITPDHPDREALRQKFLDEHTHAEDEVRFFVEGCGLFC
HHHHHHHHHEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
LHIGAEVLQVLCEQNDCINVPAGTRHWFDMGSKPQFCAVRFFDNPEGWIANFTGDAIAER
HHHHHHHHHHHHCCCCEEECCCCCCHHHCCCCCCCEEEEEEEECCCCEEEECCHHHHHHH
FAKLP
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA