Click here to switch to the map view.

The map label for this gene is masA

Identifier: 33862457

GI number: 33862457

Start: 214366

End: 215103

Strand: Reverse

Name: masA

Synonym: PMT0184

Alternate gene names: 33862457

Gene position: 215103-214366 (Counterclockwise)

Preceding gene: 33862458

Following gene: 33862452

Centisome position: 8.92

GC content: 42.28

Gene sequence:

>738_bases
GTGATCACTCACATCTTGCTTGATATTGAAGGCACCACATGCCCTACAAGCTTTGTTAGCGATACCCTCTTTCCTTACGC
TGATACCCATCTAGAAGGATTCCTAAATGAACACATCGAAAACAATGAGGTTCAATCTCTCATAGATGAAGCTTGGCATG
AATGGCAAGCTGATGAGGATCCGAGCAGTAAAGATCTCCTAAGCAAAGCATTTAGGGAGAACAGCAGCGAAATCGAGAAC
ATCTGCAGTTACCTTCACCATCTCATAACAATTGATCGCAAGTCTAGTGCTTTAAAAGATCTGCAAGGCAGAATCTGGCG
AGAGGGTTATGAGAAAGGTGACATCTCCTCTTCACTCTACCCAGAAACGATCGAGGTGCTAAACAAACTAAAACAACAAG
ATTACATCCTTGCAGTATATTCATCTGGCAGCATATCAGCTCAAAAACTCCTCTACAGACACACGACTAATGGAGATCAA
ACAGCGCTCTTTAGCCACTGGTTTGATACTCGTACTGGCAACAAGAAAGAAAGCAAAAGCTATAGCGATATCTCCATAGC
TATGAACATTCCCGTAGAAAAGGTGATGTTTGTTAGTGATAGCTGCGCAGAATGTAATGCCGCAAAAAAAGCAGGCATGA
GTGTTTTATTCAGTCTTAGGGAAGGCAATCCAGAACAAGACCCACATGATCACCAGCCAATCAAAGACCTCAGGTGTCTA
TTTGATTATCTTCTTTAA

Upstream 100 bases:

>100_bases
CTTGGGGGGAGAGCCTCAACGAAGCAAGGCGTCATGTAGAGATCTTGGAGTTCCTACTAGAGCTCTGCTGGCGTGAACAA
CTTATAGGCAGCCAAAAATC

Downstream 100 bases:

>100_bases
AAATCACACTGACTTAGCCATCAAAAAAGCCACCCCTGCGGGTGGCTTTTTCTTGGTAAATCAACAACTCTTTCGCAAGA
TTGATGATCTACATGTGGAA

Product: enolase-phosphatase E-1

Products: NA

Alternate protein names: 2,3-diketo-5-methylthio-1-phosphopentane phosphatase

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MITHILLDIEGTTCPTSFVSDTLFPYADTHLEGFLNEHIENNEVQSLIDEAWHEWQADEDPSSKDLLSKAFRENSSEIEN
ICSYLHHLITIDRKSSALKDLQGRIWREGYEKGDISSSLYPETIEVLNKLKQQDYILAVYSSGSISAQKLLYRHTTNGDQ
TALFSHWFDTRTGNKKESKSYSDISIAMNIPVEKVMFVSDSCAECNAAKKAGMSVLFSLREGNPEQDPHDHQPIKDLRCL
FDYLL

Sequences:

>Translated_245_residues
MITHILLDIEGTTCPTSFVSDTLFPYADTHLEGFLNEHIENNEVQSLIDEAWHEWQADEDPSSKDLLSKAFRENSSEIEN
ICSYLHHLITIDRKSSALKDLQGRIWREGYEKGDISSSLYPETIEVLNKLKQQDYILAVYSSGSISAQKLLYRHTTNGDQ
TALFSHWFDTRTGNKKESKSYSDISIAMNIPVEKVMFVSDSCAECNAAKKAGMSVLFSLREGNPEQDPHDHQPIKDLRCL
FDYLL
>Mature_245_residues
MITHILLDIEGTTCPTSFVSDTLFPYADTHLEGFLNEHIENNEVQSLIDEAWHEWQADEDPSSKDLLSKAFRENSSEIEN
ICSYLHHLITIDRKSSALKDLQGRIWREGYEKGDISSSLYPETIEVLNKLKQQDYILAVYSSGSISAQKLLYRHTTNGDQ
TALFSHWFDTRTGNKKESKSYSDISIAMNIPVEKVMFVSDSCAECNAAKKAGMSVLFSLREGNPEQDPHDHQPIKDLRCL
FDYLL

Specific function: Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone

COG id: COG4229

COG function: function code C; Predicted enolase-phosphatase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. MasA/mtnC family

Homologues:

Organism=Homo sapiens, GI10864017, Length=232, Percent_Identity=34.051724137931, Blast_Score=143, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI115533232, Length=220, Percent_Identity=39.5454545454545, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6320797, Length=245, Percent_Identity=31.8367346938775, Blast_Score=117, Evalue=1e-27,
Organism=Drosophila melanogaster, GI281360080, Length=219, Percent_Identity=33.7899543378995, Blast_Score=139, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24644163, Length=219, Percent_Identity=33.7899543378995, Blast_Score=139, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MTNC_PROMM (Q7V8Y7)

Other databases:

- EMBL:   BX548175
- RefSeq:   NP_894017.1
- HSSP:   P32626
- ProteinModelPortal:   Q7V8Y7
- SMR:   Q7V8Y7
- STRING:   Q7V8Y7
- GeneID:   1729665
- GenomeReviews:   BX548175_GR
- KEGG:   pmt:PMT0184
- NMPDR:   fig|74547.1.peg.184
- eggNOG:   COG4229
- HOGENOM:   HBG294242
- OMA:   TTDLNFI
- ProtClustDB:   CLSK866737
- BioCyc:   PMAR74547:PMT0184-MONOMER
- HAMAP:   MF_01681
- InterPro:   IPR005834
- InterPro:   IPR010041
- InterPro:   IPR023214
- Gene3D:   G3DSA:3.40.50.1000
- PANTHER:   PTHR20371
- TIGRFAMs:   TIGR01691

Pfam domain/function: PF00702 Hydrolase; SSF56784 SSF56784

EC number: =3.1.3.77

Molecular weight: Translated: 27913; Mature: 27913

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITHILLDIEGTTCPTSFVSDTLFPYADTHLEGFLNEHIENNEVQSLIDEAWHEWQADED
CCEEEEEEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCC
PSSKDLLSKAFRENSSEIENICSYLHHLITIDRKSSALKDLQGRIWREGYEKGDISSSLY
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
PETIEVLNKLKQQDYILAVYSSGSISAQKLLYRHTTNGDQTALFSHWFDTRTGNKKESKS
HHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHCCC
YSDISIAMNIPVEKVMFVSDSCAECNAAKKAGMSVLFSLREGNPEQDPHDHQPIKDLRCL
CCCEEEEEECCHHHHHEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
FDYLL
HHHHC
>Mature Secondary Structure
MITHILLDIEGTTCPTSFVSDTLFPYADTHLEGFLNEHIENNEVQSLIDEAWHEWQADED
CCEEEEEEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCC
PSSKDLLSKAFRENSSEIENICSYLHHLITIDRKSSALKDLQGRIWREGYEKGDISSSLY
CCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
PETIEVLNKLKQQDYILAVYSSGSISAQKLLYRHTTNGDQTALFSHWFDTRTGNKKESKS
HHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCHHCCC
YSDISIAMNIPVEKVMFVSDSCAECNAAKKAGMSVLFSLREGNPEQDPHDHQPIKDLRCL
CCCEEEEEECCHHHHHEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
FDYLL
HHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642