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The map label for this gene is ttdB

Identifier: 32267292

GI number: 32267292

Start: 1713419

End: 1713976

Strand: Direct

Name: ttdB

Synonym: HH1793

Alternate gene names: 32267292

Gene position: 1713419-1713976 (Clockwise)

Preceding gene: 32267291

Following gene: 32267293

Centisome position: 95.24

GC content: 44.44

Gene sequence:

>558_bases
ATGGGAGAGCCAAAAAAAATCACTGCACCTATAAGTAAAGAAGTGGCAAAAAGCCTCAAAGCAGGGGAGAGTGTGCTAAT
CTCTGGCACAATTTTATGCGCTCGTGATGCAGCACATAAAGCTTTAACTGAAGCGTTAGCACGTGGAGAAAAGCTCCCTG
TAGATTTGAGCGGTGAGACGATTTATTATCTAGGACCTACTCCTGCAAAGCCGGGCAATGCGATAGGTAGCGCAGGTCCT
ACGACAAGCGGGAGAATGGATAAATATACGCCGACAATCATTGAGCAAGGCATTCACGGAATGATCGGTAAAGGCTATCG
CAGCAAAGAAGTCATAGATTCTATGGTTAAGCACGGCGTTGTATATATGGTGGCTGTGGGTGGTGCGGCAGCACTCATTT
CAAAATGTATCACTAAATATGAAGTCTTAGCTTATCCTGAGTTAGGACCTGAAGCGGTTGCACGACTTACAATAGAAAAC
TTCCCTGCGATTGTGGCTATTGATGCGTCAGGCAATAATTACTACGAAGTTGGACAAGCTCCTTATAAGAAAATTTAG

Upstream 100 bases:

>100_bases
GTGGAATGGTATCCTACACACATTGCGGGGCTTCCTGTGGCAGTAAATATCAACTGCCACGCCGCACGACACGCTGATAT
TGAGCTTTAAGGAGGAAAAA

Downstream 100 bases:

>100_bases
TATTTTGTATCTTAATAGATTCTCAAATCTATTAAGATTTATGTTCAATAACAAACTCCGAAATAAATATAATGTGAATG
GGGGAATAATGTTTTTATAT

Product: fumarate hydratase

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 185; Mature: 184

Protein sequence:

>185_residues
MGEPKKITAPISKEVAKSLKAGESVLISGTILCARDAAHKALTEALARGEKLPVDLSGETIYYLGPTPAKPGNAIGSAGP
TTSGRMDKYTPTIIEQGIHGMIGKGYRSKEVIDSMVKHGVVYMVAVGGAAALISKCITKYEVLAYPELGPEAVARLTIEN
FPAIVAIDASGNNYYEVGQAPYKKI

Sequences:

>Translated_185_residues
MGEPKKITAPISKEVAKSLKAGESVLISGTILCARDAAHKALTEALARGEKLPVDLSGETIYYLGPTPAKPGNAIGSAGP
TTSGRMDKYTPTIIEQGIHGMIGKGYRSKEVIDSMVKHGVVYMVAVGGAAALISKCITKYEVLAYPELGPEAVARLTIEN
FPAIVAIDASGNNYYEVGQAPYKKI
>Mature_184_residues
GEPKKITAPISKEVAKSLKAGESVLISGTILCARDAAHKALTEALARGEKLPVDLSGETIYYLGPTPAKPGNAIGSAGPT
TSGRMDKYTPTIIEQGIHGMIGKGYRSKEVIDSMVKHGVVYMVAVGGAAALISKCITKYEVLAYPELGPEAVARLTIENF
PAIVAIDASGNNYYEVGQAPYKKI

Specific function: It functions as an aerobic enzyme in the citric acid cycle. It accounts for about 80% of the fumarase activity when the bacteria grows aerobically [H]

COG id: COG1838

COG function: function code C; Tartrate dehydratase beta subunit/Fumarate hydratase class I, C-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1787897, Length=152, Percent_Identity=42.1052631578947, Blast_Score=127, Evalue=5e-31,
Organism=Escherichia coli, GI1790564, Length=152, Percent_Identity=41.4473684210526, Blast_Score=123, Evalue=8e-30,
Organism=Escherichia coli, GI1789443, Length=174, Percent_Identity=33.3333333333333, Blast_Score=91, Evalue=4e-20,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 19451; Mature: 19320

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEPKKITAPISKEVAKSLKAGESVLISGTILCARDAAHKALTEALARGEKLPVDLSGET
CCCCCCEECCHHHHHHHHHHCCCEEEEECEEEECCHHHHHHHHHHHHCCCCCCEEECCCE
IYYLGPTPAKPGNAIGSAGPTTSGRMDKYTPTIIEQGIHGMIGKGYRSKEVIDSMVKHGV
EEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCE
VYMVAVGGAAALISKCITKYEVLAYPELGPEAVARLTIENFPAIVAIDASGNNYYEVGQA
EEEEECCCHHHHHHHHHHHHHEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCEEECCCC
PYKKI
CCCCC
>Mature Secondary Structure 
GEPKKITAPISKEVAKSLKAGESVLISGTILCARDAAHKALTEALARGEKLPVDLSGET
CCCCCEECCHHHHHHHHHHCCCEEEEECEEEECCHHHHHHHHHHHHCCCCCCEEECCCE
IYYLGPTPAKPGNAIGSAGPTTSGRMDKYTPTIIEQGIHGMIGKGYRSKEVIDSMVKHGV
EEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHCCE
VYMVAVGGAAALISKCITKYEVLAYPELGPEAVARLTIENFPAIVAIDASGNNYYEVGQA
EEEEECCCHHHHHHHHHHHHHEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCEEECCCC
PYKKI
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 1879695 [H]