Click here to switch to the map view.

The map label for this gene is rlmB [H]

Identifier: 32267245

GI number: 32267245

Start: 1671835

End: 1672524

Strand: Direct

Name: rlmB [H]

Synonym: HH1746

Alternate gene names: 32267245

Gene position: 1671835-1672524 (Clockwise)

Preceding gene: 32267244

Following gene: 32267246

Centisome position: 92.92

GC content: 36.09

Gene sequence:

>690_bases
GTGATAGTTTATGGAAAACAACCCATTTTGTATATTTTACACACAAATGCTTCGTGCATTGAAGAAATATATCTTGCTAA
AGAGTTACCAAAGGACATATTTAAACAACTTGCGCAATTAGATAAGCCTATAAAACGTGTAGATATGAAAAAAGCACAAG
CTCTTGCTCACGGAGGGAATCATCAAGGCATTTTGGCTTCTATTATACCACCAAGTCCGCTTAGCTTGAATGATCTCAAA
AGCAAAGATTCTTTACTTGTGCTTTATAATGTTACTGATGTAGGGAATATCGGTTCAATTTTTCGCACTGCTTATGCTTT
AGGCGTGGGAGGGGTGGTTATTTGTTTATCAAACCTTAGCCAAAAAGCCATAGAAAGTATAGCTCGTCTTTCAAGCGGTG
CTTTTTTACATATGCCTTATGGTGTTTTTCCGCATATCTTTGATATAATAAATGAATTAAAAAATGCAGATTTTATGCTC
ATAGGTGCAGATATGCAAGGGCAGGAGAGCTGTAACGTAAAAAAGAAATGGGCTTTATTTTTAGGGAGTGAATCTGAAGG
TTTATCAGCGCGATTGAAGCACAAACTTGATACAATCCTCTCCATAAAAATGGAGCACCATTTTGATTCACTCAATGTGG
CAGTAGCCACGGGAATTTTAATAGATAGGATTAAAAATGCAAGGAAATAA

Upstream 100 bases:

>100_bases
TTTATACTTGATATTCCACCAAAGATTAAAGCTAAAATCTTAAGCAAAATAAGCGGACATAGCATTAAGCAATGTTATGA
AGAAATACTAAGGTGAATCA

Downstream 100 bases:

>100_bases
TACAAATACTACATTTCTTGATGAAGAACTTGCCAAATTGAAGGCTATTGGATTAAAGGAAATTAGCAAAAACACTAAAC
TTGCAAGCTCAAAGATTGAA

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MIVYGKQPILYILHTNASCIEEIYLAKELPKDIFKQLAQLDKPIKRVDMKKAQALAHGGNHQGILASIIPPSPLSLNDLK
SKDSLLVLYNVTDVGNIGSIFRTAYALGVGGVVICLSNLSQKAIESIARLSSGAFLHMPYGVFPHIFDIINELKNADFML
IGADMQGQESCNVKKKWALFLGSESEGLSARLKHKLDTILSIKMEHHFDSLNVAVATGILIDRIKNARK

Sequences:

>Translated_229_residues
MIVYGKQPILYILHTNASCIEEIYLAKELPKDIFKQLAQLDKPIKRVDMKKAQALAHGGNHQGILASIIPPSPLSLNDLK
SKDSLLVLYNVTDVGNIGSIFRTAYALGVGGVVICLSNLSQKAIESIARLSSGAFLHMPYGVFPHIFDIINELKNADFML
IGADMQGQESCNVKKKWALFLGSESEGLSARLKHKLDTILSIKMEHHFDSLNVAVATGILIDRIKNARK
>Mature_229_residues
MIVYGKQPILYILHTNASCIEEIYLAKELPKDIFKQLAQLDKPIKRVDMKKAQALAHGGNHQGILASIIPPSPLSLNDLK
SKDSLLVLYNVTDVGNIGSIFRTAYALGVGGVVICLSNLSQKAIESIARLSSGAFLHMPYGVFPHIFDIINELKNADFML
IGADMQGQESCNVKKKWALFLGSESEGLSARLKHKLDTILSIKMEHHFDSLNVAVATGILIDRIKNARK

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790623, Length=233, Percent_Identity=25.3218884120172, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 25179; Mature: 25179

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVYGKQPILYILHTNASCIEEIYLAKELPKDIFKQLAQLDKPIKRVDMKKAQALAHGGN
CEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
HQGILASIIPPSPLSLNDLKSKDSLLVLYNVTDVGNIGSIFRTAYALGVGGVVICLSNLS
CCCEEEEECCCCCCCHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
QKAIESIARLSSGAFLHMPYGVFPHIFDIINELKNADFMLIGADMQGQESCNVKKKWALF
HHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEE
LGSESEGLSARLKHKLDTILSIKMEHHFDSLNVAVATGILIDRIKNARK
ECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIVYGKQPILYILHTNASCIEEIYLAKELPKDIFKQLAQLDKPIKRVDMKKAQALAHGGN
CEEECCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
HQGILASIIPPSPLSLNDLKSKDSLLVLYNVTDVGNIGSIFRTAYALGVGGVVICLSNLS
CCCEEEEECCCCCCCHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHH
QKAIESIARLSSGAFLHMPYGVFPHIFDIINELKNADFMLIGADMQGQESCNVKKKWALF
HHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEE
LGSESEGLSARLKHKLDTILSIKMEHHFDSLNVAVATGILIDRIKNARK
ECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]