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The map label for this gene is ispB [H]

Identifier: 32267223

GI number: 32267223

Start: 1649171

End: 1650082

Strand: Direct

Name: ispB [H]

Synonym: HH1724

Alternate gene names: 32267223

Gene position: 1649171-1650082 (Clockwise)

Preceding gene: 32267222

Following gene: 32267224

Centisome position: 91.66

GC content: 34.1

Gene sequence:

>912_bases
GTGAATATACAATTTGCACTTGAACGCATTAAGGCATATATTCATACTTACGTCCAAGAGTGTAAAAATGAAGAAGTAAA
TGCTTTATATGGACATTTAAGTTATGGGAAAATGCTTCGTAGCAAACTTTTGCTTTCTATTAGTGATATGAACGAAGATG
CTTTAAGAGTGTGTGCCTTAATTGAAATGATACAAAGTGCCTCACTTTTGCACGATGATGTGATTGATGAATCTCTTACA
AGACGCGGTATTCCATCTATTAATGCTACTTTTGGTAATAAAAATGCCATAATGCTGGGCGATGTGCTTTATTCTAAGGC
ATTTTATGAGCTAAGCTGTCTTGATGTCAGATTAGCTCAAAGTGTTTCTTCAGCTGTTGTTCGGCTTTCTGTGGGTGAAA
TTGAAGATGTAAAACTTTCACTTTCTTTTCATACAGATAAGGAGCGATATATTCGTATGTGTGCAGATAAAACAGCCGCA
CTTATTGTGGCTGCTGCGGAATGTGGTGCGATTTTAAAGGGTTTAAATAAAAATATTTATAGAATCTATGGTGAAAATTT
AGGCATTGCTTTTCAAATAATTGATGATATTTTAGATATCACTCAAGATTCCCAAACACTTGGTAAGCCTTCAATGAGTG
ATTTTAAAGAAGGTAAAACGACTCTTCCTTATATGTATTTATATGAACATTTAAATGCAGATGAGCGTTCAAAATTGCTT
GAGTGCTATGGTGTGCATCTTAATGAGGAAAAACAAGCGTGGATAAGAATGCAATTGCAAAAATATAATATCATCGCTCA
AAGTGTCGCAGATGCTCAATATTATGCTAATAAAGCCATTCAATCTATTGTAAATGAGGGCAATGAGAAGCTTAAAGATA
TTGTTACACAAATGATTGAGAGGACATTTTAG

Upstream 100 bases:

>100_bases
AGGGGCAAGGTGCAGCAGTGCTTGATGAGAGCATTTCGCCAAGTAAAGATAAAAAAGAAGACGAAGAGGCAGAAGAAGAT
ACTACTCAAGGACAAGCATC

Downstream 100 bases:

>100_bases
GGAGGATAGAATGCAAGAAAAAATGGAAGTGCAATATATGGTGGTAAGTTTTTCACACAAAAATGTAGATATTGCTACGC
GTGAGAAACTGAGTTTTAGC

Product: hypothetical protein

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MNIQFALERIKAYIHTYVQECKNEEVNALYGHLSYGKMLRSKLLLSISDMNEDALRVCALIEMIQSASLLHDDVIDESLT
RRGIPSINATFGNKNAIMLGDVLYSKAFYELSCLDVRLAQSVSSAVVRLSVGEIEDVKLSLSFHTDKERYIRMCADKTAA
LIVAAAECGAILKGLNKNIYRIYGENLGIAFQIIDDILDITQDSQTLGKPSMSDFKEGKTTLPYMYLYEHLNADERSKLL
ECYGVHLNEEKQAWIRMQLQKYNIIAQSVADAQYYANKAIQSIVNEGNEKLKDIVTQMIERTF

Sequences:

>Translated_303_residues
MNIQFALERIKAYIHTYVQECKNEEVNALYGHLSYGKMLRSKLLLSISDMNEDALRVCALIEMIQSASLLHDDVIDESLT
RRGIPSINATFGNKNAIMLGDVLYSKAFYELSCLDVRLAQSVSSAVVRLSVGEIEDVKLSLSFHTDKERYIRMCADKTAA
LIVAAAECGAILKGLNKNIYRIYGENLGIAFQIIDDILDITQDSQTLGKPSMSDFKEGKTTLPYMYLYEHLNADERSKLL
ECYGVHLNEEKQAWIRMQLQKYNIIAQSVADAQYYANKAIQSIVNEGNEKLKDIVTQMIERTF
>Mature_303_residues
MNIQFALERIKAYIHTYVQECKNEEVNALYGHLSYGKMLRSKLLLSISDMNEDALRVCALIEMIQSASLLHDDVIDESLT
RRGIPSINATFGNKNAIMLGDVLYSKAFYELSCLDVRLAQSVSSAVVRLSVGEIEDVKLSLSFHTDKERYIRMCADKTAA
LIVAAAECGAILKGLNKNIYRIYGENLGIAFQIIDDILDITQDSQTLGKPSMSDFKEGKTTLPYMYLYEHLNADERSKLL
ECYGVHLNEEKQAWIRMQLQKYNIIAQSVADAQYYANKAIQSIVNEGNEKLKDIVTQMIERTF

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=257, Percent_Identity=31.1284046692607, Blast_Score=107, Evalue=1e-23,
Organism=Escherichia coli, GI1789578, Length=201, Percent_Identity=38.3084577114428, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1786623, Length=230, Percent_Identity=32.6086956521739, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17505681, Length=183, Percent_Identity=37.1584699453552, Blast_Score=113, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6319475, Length=277, Percent_Identity=31.7689530685921, Blast_Score=115, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24651612, Length=190, Percent_Identity=35.7894736842105, Blast_Score=106, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 34293; Mature: 34293

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIQFALERIKAYIHTYVQECKNEEVNALYGHLSYGKMLRSKLLLSISDMNEDALRVCAL
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
IEMIQSASLLHDDVIDESLTRRGIPSINATFGNKNAIMLGDVLYSKAFYELSCLDVRLAQ
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
SVSSAVVRLSVGEIEDVKLSLSFHTDKERYIRMCADKTAALIVAAAECGAILKGLNKNIY
HHHHHHHHHCCCCCEEEEEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEE
RIYGENLGIAFQIIDDILDITQDSQTLGKPSMSDFKEGKTTLPYMYLYEHLNADERSKLL
EEECCCCCHHHHHHHHHHHHCCCCHHCCCCCHHHHHCCCCCCHHHHHHHHCCCHHHHHHH
ECYGVHLNEEKQAWIRMQLQKYNIIAQSVADAQYYANKAIQSIVNEGNEKLKDIVTQMIE
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
RTF
HCC
>Mature Secondary Structure
MNIQFALERIKAYIHTYVQECKNEEVNALYGHLSYGKMLRSKLLLSISDMNEDALRVCAL
CCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
IEMIQSASLLHDDVIDESLTRRGIPSINATFGNKNAIMLGDVLYSKAFYELSCLDVRLAQ
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHH
SVSSAVVRLSVGEIEDVKLSLSFHTDKERYIRMCADKTAALIVAAAECGAILKGLNKNIY
HHHHHHHHHCCCCCEEEEEEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEE
RIYGENLGIAFQIIDDILDITQDSQTLGKPSMSDFKEGKTTLPYMYLYEHLNADERSKLL
EEECCCCCHHHHHHHHHHHHCCCCHHCCCCCHHHHHCCCCCCHHHHHHHHCCCHHHHHHH
ECYGVHLNEEKQAWIRMQLQKYNIIAQSVADAQYYANKAIQSIVNEGNEKLKDIVTQMIE
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
RTF
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]