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The map label for this gene is lacA [C]

Identifier: 32266968

GI number: 32266968

Start: 1410599

End: 1411216

Strand: Direct

Name: lacA [C]

Synonym: HH1469

Alternate gene names: 32266968

Gene position: 1410599-1411216 (Clockwise)

Preceding gene: 32266967

Following gene: 32266969

Centisome position: 78.4

GC content: 43.2

Gene sequence:

>618_bases
ATGAAACCAACTTTTTCGCTTCGTAGCACACCCACACCTAGCACAAATCGCGATATTTTCACGCGGGATTTGGGTGGGGA
GCTTATAGATATGAATGACCCAGAGTTTGAGAGGATTAAAGAGATTATTTTAGAGACGATTAGGCTTACAAGTGAGCTAA
ATAGCGGACATCATAGCGCCGAGCGCGTGCAGGAGCTGTTTTCGCAAATCATTGGCAAAGAGGTGGATTGTAGCACTTGG
ATTATCCCGCCTTTTTATGTGGATTTTGGCAAAAATATTAAGGTGGGCAAAAATTTCTTTATGAATCAAGCTTGCACCTT
TATGGATAGGGGCGGCATCACAATCGGTGATGATGTGTTTATCGCACCCAAAGTCTGCCTCACAACGATTAATCACGATT
TTAATCCCTACAATCGCCAAGCGACTTTTTGCAAACCCATTGTGATAAAAGATAGGGTATGGATAGGGATTAATGCAACT
ATTTGTCCGGGTGTAACCATTGGGCAAAACTCTGTGATTGCTGCTGGAAGCGTGGTAACTAAAGATGTTCCTCCAAATGT
GATTGTTGGAGGCAACCCTGCAAGGGTGCTAAAAAGCATTGAGGGGTATGAGAAATAG

Upstream 100 bases:

>100_bases
AGGTGCTTAATGCGGTGTTTGTAGCTAAAAAAGTTTTTAAGGAGAGGAGAAAATAATAAAGAAGCAACACAAAAGAATTT
TTATATTTTAGGGAGAAACT

Downstream 100 bases:

>100_bases
CCACATAAAGCAATAACAAGTTTCTACATCTTAAGGGCATTTATAGGCAGGTAGAAATTTTATATTCAGCCAAAACTGCT
ATAATAAACACAAGTTAGAT

Product: hypothetical protein

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MKPTFSLRSTPTPSTNRDIFTRDLGGELIDMNDPEFERIKEIILETIRLTSELNSGHHSAERVQELFSQIIGKEVDCSTW
IIPPFYVDFGKNIKVGKNFFMNQACTFMDRGGITIGDDVFIAPKVCLTTINHDFNPYNRQATFCKPIVIKDRVWIGINAT
ICPGVTIGQNSVIAAGSVVTKDVPPNVIVGGNPARVLKSIEGYEK

Sequences:

>Translated_205_residues
MKPTFSLRSTPTPSTNRDIFTRDLGGELIDMNDPEFERIKEIILETIRLTSELNSGHHSAERVQELFSQIIGKEVDCSTW
IIPPFYVDFGKNIKVGKNFFMNQACTFMDRGGITIGDDVFIAPKVCLTTINHDFNPYNRQATFCKPIVIKDRVWIGINAT
ICPGVTIGQNSVIAAGSVVTKDVPPNVIVGGNPARVLKSIEGYEK
>Mature_205_residues
MKPTFSLRSTPTPSTNRDIFTRDLGGELIDMNDPEFERIKEIILETIRLTSELNSGHHSAERVQELFSQIIGKEVDCSTW
IIPPFYVDFGKNIKVGKNFFMNQACTFMDRGGITIGDDVFIAPKVCLTTINHDFNPYNRQATFCKPIVIKDRVWIGINAT
ICPGVTIGQNSVIAAGSVVTKDVPPNVIVGGNPARVLKSIEGYEK

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=190, Percent_Identity=39.4736842105263, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI1786664, Length=181, Percent_Identity=40.8839779005525, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1788367, Length=136, Percent_Identity=33.8235294117647, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI1788345, Length=97, Percent_Identity=37.1134020618557, Blast_Score=62, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322243, Length=179, Percent_Identity=40.7821229050279, Blast_Score=127, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 22763; Mature: 22763

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPTFSLRSTPTPSTNRDIFTRDLGGELIDMNDPEFERIKEIILETIRLTSELNSGHHSA
CCCCCCCCCCCCCCCCCCEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHH
ERVQELFSQIIGKEVDCSTWIIPPFYVDFGKNIKVGKNFFMNQACTFMDRGGITIGDDVF
HHHHHHHHHHHCCCCCCCEEECCCCEEECCCCEECCHHHHHHHHHHHHHCCCEEECCCEE
IAPKVCLTTINHDFNPYNRQATFCKPIVIKDRVWIGINATICPGVTIGQNSVIAAGSVVT
ECCHHHEEECCCCCCCCCCCCCEECCEEEECEEEEEECCEECCCEEECCCCEEECCCEEE
KDVPPNVIVGGNPARVLKSIEGYEK
CCCCCCEEECCCHHHHHHHHHCCCC
>Mature Secondary Structure
MKPTFSLRSTPTPSTNRDIFTRDLGGELIDMNDPEFERIKEIILETIRLTSELNSGHHSA
CCCCCCCCCCCCCCCCCCEEEECCCCEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHH
ERVQELFSQIIGKEVDCSTWIIPPFYVDFGKNIKVGKNFFMNQACTFMDRGGITIGDDVF
HHHHHHHHHHHCCCCCCCEEECCCCEEECCCCEECCHHHHHHHHHHHHHCCCEEECCCEE
IAPKVCLTTINHDFNPYNRQATFCKPIVIKDRVWIGINATICPGVTIGQNSVIAAGSVVT
ECCHHHEEECCCCCCCCCCCCCEECCEEEECEEEEEECCEECCCEEECCCCEEECCCEEE
KDVPPNVIVGGNPARVLKSIEGYEK
CCCCCCEEECCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA