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The map label for this gene is arsB [H]

Identifier: 32266923

GI number: 32266923

Start: 1367061

End: 1368368

Strand: Direct

Name: arsB [H]

Synonym: HH1424

Alternate gene names: 32266923

Gene position: 1367061-1368368 (Clockwise)

Preceding gene: 32266922

Following gene: 32266924

Centisome position: 75.98

GC content: 37.16

Gene sequence:

>1308_bases
ATGGATTTTATGGCTTTTAGTCTTTTTATACTCACAATGCTTCTTATTTATTGGCGTCCTTTAAATCTTCCTTTGTGGGT
GTTTAGCACTTTAGGAGCTGTAGGCGCTTATTTATTCAATGTAGTGAATTTGGACGATATTTGGCGTGTATGGGCAATGG
TGTGGGATAGCACACTTACACTCATTGGACTTATCCTTCTTACTCTCGCGTTTGAAACATTAGGATTTTTTGATTTCCTT
GCATTATTACTTATCCGAATCTTAAGTCAAAAGGGTGAGAAACAAAGCTTAAGTAGCGCAAGATTCTATATTTTTCTTGT
GCTTTTTAGTGCTTTTTTAAGTGCGTTTTTTGCAAACGATGGTGCTATTTTAATCCTCACACCTTTAATCCTCACACTTT
TTAAAGTGCATAATACAAAGGTATTTTTCACGCCTTTAATTGTATTTTTGTTTCTTGTAAGCTTTGTGAGTGATTTTGCT
TCAAATACCTTTGTTATTTCTAATCTCACAAACATTATCACCGCGCATTTTTTTGAGATTCCCTCACTCCGCTTTAGCGT
GCTTATGTTTTTGCCCCAAATTGTTGTGATTTGTGGGATTGTAGGATTTTGGTATATAGTGAGGAGAATCTTGCCTCCTA
GACTTGAATTTAGGGATTTGCAAAGTGATATTTCTAAAGGTTGCATTATCTTTTGTTTTGCCCTGCTACTCCTTCTTGTG
TTTGGTATTGCTTTTGCGCATAAATTAGGTATTCCACTCTCAAGTTTTACATTTCTATGTGCGATTTTGGCATTGCTTTA
TGGCAAGATAAAAGGGCAGCTAGGGATATTCTCTTGTTTTAAAAACGCACCCTTTGGTATAGTATTATTTTCTCTTGGGC
TATTTGTTGTCGTTTTTGGGCTTAAAAATGGCGGTGTGTTGCAGATTCTAAGTGATAATCTGTCGTTGTTTGTCTCGTTG
CCAAAAGAATGGCAGATTGTAAGTATGGGGCTTGGAAGTGGTGTTGGCAGTAGTGTGATAAACAATCTGCCTATGGTAAT
GCTTGGTAATCTCGCACTTGCAGATTTTAAAGCATATTTGAATGAGGCGCAGGAAAGTCTTATCATCGCACATTTGCTTG
GTTGCAATATTGGCTCGAAACTCACACCCATAGGCTCACTTGCAACTTTACTTTGGCTTGAAAGGTTAAAAGGCTATGGA
ATCCGCATTAGTTTTATGCGTTATATGGCAGTGGCGTTTGTAGTTACTCTACCTGTGCTATTTTTAGGATTGTTCGGGCT
GATTTGGGGATATTATTTTTATGTTTGA

Upstream 100 bases:

>100_bases
GCAGTTGAAGCTAAGTTAATCTTAACTCTTCTTAATACCCTCTATGTTAGAATCTCGCAAAAATTGCAAATCAAAGCAGG
TATGAACGAGGTAGCCTTTT

Downstream 100 bases:

>100_bases
AAAATAGGCTTTTATAAAAAGTTACTTAGATTCACTATAATAGAAGTAATCAAAACGATTGGGAAATAAGAGTGAAAAAG
TCTAAAACAATAAAAATTAT

Product: hypothetical protein

Products: ADP; phosphate; arsenite [Periplasm] [C]

Alternate protein names: Arsenic efflux pump protein [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MDFMAFSLFILTMLLIYWRPLNLPLWVFSTLGAVGAYLFNVVNLDDIWRVWAMVWDSTLTLIGLILLTLAFETLGFFDFL
ALLLIRILSQKGEKQSLSSARFYIFLVLFSAFLSAFFANDGAILILTPLILTLFKVHNTKVFFTPLIVFLFLVSFVSDFA
SNTFVISNLTNIITAHFFEIPSLRFSVLMFLPQIVVICGIVGFWYIVRRILPPRLEFRDLQSDISKGCIIFCFALLLLLV
FGIAFAHKLGIPLSSFTFLCAILALLYGKIKGQLGIFSCFKNAPFGIVLFSLGLFVVVFGLKNGGVLQILSDNLSLFVSL
PKEWQIVSMGLGSGVGSSVINNLPMVMLGNLALADFKAYLNEAQESLIIAHLLGCNIGSKLTPIGSLATLLWLERLKGYG
IRISFMRYMAVAFVVTLPVLFLGLFGLIWGYYFYV

Sequences:

>Translated_435_residues
MDFMAFSLFILTMLLIYWRPLNLPLWVFSTLGAVGAYLFNVVNLDDIWRVWAMVWDSTLTLIGLILLTLAFETLGFFDFL
ALLLIRILSQKGEKQSLSSARFYIFLVLFSAFLSAFFANDGAILILTPLILTLFKVHNTKVFFTPLIVFLFLVSFVSDFA
SNTFVISNLTNIITAHFFEIPSLRFSVLMFLPQIVVICGIVGFWYIVRRILPPRLEFRDLQSDISKGCIIFCFALLLLLV
FGIAFAHKLGIPLSSFTFLCAILALLYGKIKGQLGIFSCFKNAPFGIVLFSLGLFVVVFGLKNGGVLQILSDNLSLFVSL
PKEWQIVSMGLGSGVGSSVINNLPMVMLGNLALADFKAYLNEAQESLIIAHLLGCNIGSKLTPIGSLATLLWLERLKGYG
IRISFMRYMAVAFVVTLPVLFLGLFGLIWGYYFYV
>Mature_435_residues
MDFMAFSLFILTMLLIYWRPLNLPLWVFSTLGAVGAYLFNVVNLDDIWRVWAMVWDSTLTLIGLILLTLAFETLGFFDFL
ALLLIRILSQKGEKQSLSSARFYIFLVLFSAFLSAFFANDGAILILTPLILTLFKVHNTKVFFTPLIVFLFLVSFVSDFA
SNTFVISNLTNIITAHFFEIPSLRFSVLMFLPQIVVICGIVGFWYIVRRILPPRLEFRDLQSDISKGCIIFCFALLLLLV
FGIAFAHKLGIPLSSFTFLCAILALLYGKIKGQLGIFSCFKNAPFGIVLFSLGLFVVVFGLKNGGVLQILSDNLSLFVSL
PKEWQIVSMGLGSGVGSSVINNLPMVMLGNLALADFKAYLNEAQESLIIAHLLGCNIGSKLTPIGSLATLLWLERLKGYG
IRISFMRYMAVAFVVTLPVLFLGLFGLIWGYYFYV

Specific function: Involved in arsenical resistance. Thought to form the channel of an arsenite pump [H]

COG id: COG1055

COG function: function code P; Na+/H+ antiporter NhaD and related arsenite permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ArsB family [H]

Homologues:

Organism=Escherichia coli, GI87082277, Length=429, Percent_Identity=32.6340326340326, Blast_Score=196, Evalue=4e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000802 [H]

Pfam domain/function: PF02040 ArsB [H]

EC number: NA

Molecular weight: Translated: 48637; Mature: 48637

Theoretical pI: Translated: 9.13; Mature: 9.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arsenite [Cytoplasm]; H2O [C]

Specific reaction: ATP + arsenite [Cytoplasm] + H2O = ADP + phosphate + arsenite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12950922 [H]