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The map label for this gene is pcrA [H]

Identifier: 32266857

GI number: 32266857

Start: 1313613

End: 1315727

Strand: Direct

Name: pcrA [H]

Synonym: HH1358

Alternate gene names: 32266857

Gene position: 1313613-1315727 (Clockwise)

Preceding gene: 32266856

Following gene: 32266858

Centisome position: 73.01

GC content: 35.32

Gene sequence:

>2115_bases
ATGCCGGTATCTCTCTCATCTGTTCAACCACTTGTTCCACAAACACTTGAGCAAACTATTGTTTCGCTTAATGACGCTCA
AAAGCAAGCAGTTGAACATATTGATGGGGCAATGCTTATCCTTGCAGGAGCAGGGAGTGGTAAGACTAAAACAATTACAA
CACGTTTAGCTTATCTGATTGATGAGGTTGGGATTCCCCCAAGTGCCACACTTACACTTACCTTCACTAATAAAGCTGCG
CAGGAGATGAGAGAGAGAGCCTTAACACTTTTGCATAATAGATATGAATCTCCACCGCTTTTATGCACTTTTCATCGTTT
TGGACTACTTTTTTTACGCTTTCATATTACTGCACTTGGGAGAAATGCAAATTTTGTAGTGCTTGATACTGATGATAAAC
GCGCTATTTTAAAATCTTTGTGCAAGATTCGTCCTATTTCACAGCTTGATTATTATATTTCAGATATGAAAAATGCCTTT
CTTTCGCCCGAACAAGCACAAACTTATGCCCATAGTGAAGAATATAAGCTAATGAATAGAATCTATGGGGAATATGAAAA
TTATTTATTGAATAAAAATTTACTTGATTTTGATGATTTGCTTTATCTTACGTATGCAATTTTGCTTAAAAATGACAATA
TAGCAAAGCAAATGAGCGAAAAATATCACTACATTATGGTTGATGAATACCAAGATACTAATGAGATTCAATATAAAATC
CTTAAACGTTTATGTCTTGCTCATACTAATCTTTGTGTTGTAGGTGATGATGATCAGAGTATTTATGGGTGGCGTGGAGC
AAATGTGAAGAATATCTTAGGATTTCCTAAGGAATTTGATGAAGTAAAGGTTGTTAAGTTAGAGGAAAATTATCGCTCAA
GTGAGCAGATTCTTAATGCTGCAAATGCACTTATTTCTCACAATAGTAAGCGTTTGGGTAAAAATTTAAAGAGTATCAAA
GGTGCTGGAGCAAATGTAAGAGTAATAGAAAGTGAAAATGAAAGCACAGAAGCGGAATTTCTTGCTAAAGATATTTTAAC
GCTCCTTTCTCAAGGTATAAATCCGAGCGAAATTGCAGTTTTGTTTCGTGTAAATGCACTCTCTCGGAGTATTGAAGAGG
GCTTTAATCGTGCTAAGATTCCCTATAAAATTATTGGAGCAGTGCGCTTTTATGAACGAGCAGAGATTAAAGATTTACTT
TGCTATTTGCGATTAATTGTTAATCCTAATGATGATTATTCATTTTTACGCATTATTAATCGCCCTAGACGAGGGATTGG
TAAGATTACACAACTTAAATTAGAAGAACTTGCGCGAACATTCAATACCTCTTGTTTTTCAAGTATTACCCAATATACAC
AGCAGAGCAAAGAAGCCATTGGAGAAAAGGCATATAATGCACTTTTGGAGCTTACTCAAAATATTGAACGTTGGCGTAAA
TATACAGATTTAGCAGATGTGATTGAAGATATGCAAGAGCATATTGTATTTACATTCAGTAGGCTTGATGAGGTAGATAG
AAAGGGAAATATTGAGGAATTTTATGGTATGTTTCGTGATTATGCTATTGCTAATCCAAGCAATCAAGTTGAAGATTTTT
TAAATGATATTGCTTTGGCAAGTCCTACTGATGAATTGACAGGAGAATATGTGAGTTGTATGAGCATACATATGGCTAAG
GGATTAGAGTTTAAATATGTATATGTGATTGGTTTTGAAGAGGGTATTTTTCCTCTTTGGAATGATGAAGATGAAATACA
AGAAGAGAGACGATTAGGCTATGTGGCAATCACAAGAGCGAAAGATTTTCTTACATTATGTTCGGCAAAAAGCAGATTGC
GTAGGGGTAAAAGAGAATGGCTTGAACAATCGCGTTTTTTAAGAGAAAGTGGATTATTGGGCGTATATAATGCACCAAGT
CTTTATAAAACTCAATCTATACAAGAAGAAGCAGAAGTGGTATTTAGCAAAGGTATGAAAGTGCAACATAAACTCTTTGG
TGTAGGTGTAGTAGAATCTATAAAGGGTAAGGGAAGTCAGTGTCAGCTTAGGATTAATTTTTCCGGATTAGAGCGCGTGA
TTCTAGCTTCATTTGTGCAAAAGGTATGCGAATGA

Upstream 100 bases:

>100_bases
GTTTGGAAAAAGCTAAGCGATGAAGAAGTTAAGCCTTATCTATCCCAAAAGCCAGATTTTTCTGTTGTAGAAAAAAGTCT
TAAAGATATAAAACCATAAG

Downstream 100 bases:

>100_bases
AACAATTTTTTCTTTTTTTTGTCATTTTTGCGAATATAAATCTAATCGCAGATGATATTTCTATCCCTCCATCTAAACTC
TCATTGCAAAAGAGCTATCA

Product: DNA helicase II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 704; Mature: 703

Protein sequence:

>704_residues
MPVSLSSVQPLVPQTLEQTIVSLNDAQKQAVEHIDGAMLILAGAGSGKTKTITTRLAYLIDEVGIPPSATLTLTFTNKAA
QEMRERALTLLHNRYESPPLLCTFHRFGLLFLRFHITALGRNANFVVLDTDDKRAILKSLCKIRPISQLDYYISDMKNAF
LSPEQAQTYAHSEEYKLMNRIYGEYENYLLNKNLLDFDDLLYLTYAILLKNDNIAKQMSEKYHYIMVDEYQDTNEIQYKI
LKRLCLAHTNLCVVGDDDQSIYGWRGANVKNILGFPKEFDEVKVVKLEENYRSSEQILNAANALISHNSKRLGKNLKSIK
GAGANVRVIESENESTEAEFLAKDILTLLSQGINPSEIAVLFRVNALSRSIEEGFNRAKIPYKIIGAVRFYERAEIKDLL
CYLRLIVNPNDDYSFLRIINRPRRGIGKITQLKLEELARTFNTSCFSSITQYTQQSKEAIGEKAYNALLELTQNIERWRK
YTDLADVIEDMQEHIVFTFSRLDEVDRKGNIEEFYGMFRDYAIANPSNQVEDFLNDIALASPTDELTGEYVSCMSIHMAK
GLEFKYVYVIGFEEGIFPLWNDEDEIQEERRLGYVAITRAKDFLTLCSAKSRLRRGKREWLEQSRFLRESGLLGVYNAPS
LYKTQSIQEEAEVVFSKGMKVQHKLFGVGVVESIKGKGSQCQLRINFSGLERVILASFVQKVCE

Sequences:

>Translated_704_residues
MPVSLSSVQPLVPQTLEQTIVSLNDAQKQAVEHIDGAMLILAGAGSGKTKTITTRLAYLIDEVGIPPSATLTLTFTNKAA
QEMRERALTLLHNRYESPPLLCTFHRFGLLFLRFHITALGRNANFVVLDTDDKRAILKSLCKIRPISQLDYYISDMKNAF
LSPEQAQTYAHSEEYKLMNRIYGEYENYLLNKNLLDFDDLLYLTYAILLKNDNIAKQMSEKYHYIMVDEYQDTNEIQYKI
LKRLCLAHTNLCVVGDDDQSIYGWRGANVKNILGFPKEFDEVKVVKLEENYRSSEQILNAANALISHNSKRLGKNLKSIK
GAGANVRVIESENESTEAEFLAKDILTLLSQGINPSEIAVLFRVNALSRSIEEGFNRAKIPYKIIGAVRFYERAEIKDLL
CYLRLIVNPNDDYSFLRIINRPRRGIGKITQLKLEELARTFNTSCFSSITQYTQQSKEAIGEKAYNALLELTQNIERWRK
YTDLADVIEDMQEHIVFTFSRLDEVDRKGNIEEFYGMFRDYAIANPSNQVEDFLNDIALASPTDELTGEYVSCMSIHMAK
GLEFKYVYVIGFEEGIFPLWNDEDEIQEERRLGYVAITRAKDFLTLCSAKSRLRRGKREWLEQSRFLRESGLLGVYNAPS
LYKTQSIQEEAEVVFSKGMKVQHKLFGVGVVESIKGKGSQCQLRINFSGLERVILASFVQKVCE
>Mature_703_residues
PVSLSSVQPLVPQTLEQTIVSLNDAQKQAVEHIDGAMLILAGAGSGKTKTITTRLAYLIDEVGIPPSATLTLTFTNKAAQ
EMRERALTLLHNRYESPPLLCTFHRFGLLFLRFHITALGRNANFVVLDTDDKRAILKSLCKIRPISQLDYYISDMKNAFL
SPEQAQTYAHSEEYKLMNRIYGEYENYLLNKNLLDFDDLLYLTYAILLKNDNIAKQMSEKYHYIMVDEYQDTNEIQYKIL
KRLCLAHTNLCVVGDDDQSIYGWRGANVKNILGFPKEFDEVKVVKLEENYRSSEQILNAANALISHNSKRLGKNLKSIKG
AGANVRVIESENESTEAEFLAKDILTLLSQGINPSEIAVLFRVNALSRSIEEGFNRAKIPYKIIGAVRFYERAEIKDLLC
YLRLIVNPNDDYSFLRIINRPRRGIGKITQLKLEELARTFNTSCFSSITQYTQQSKEAIGEKAYNALLELTQNIERWRKY
TDLADVIEDMQEHIVFTFSRLDEVDRKGNIEEFYGMFRDYAIANPSNQVEDFLNDIALASPTDELTGEYVSCMSIHMAKG
LEFKYVYVIGFEEGIFPLWNDEDEIQEERRLGYVAITRAKDFLTLCSAKSRLRRGKREWLEQSRFLRESGLLGVYNAPSL
YKTQSIQEEAEVVFSKGMKVQHKLFGVGVVESIKGKGSQCQLRINFSGLERVILASFVQKVCE

Specific function: Essential helicase [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=638, Percent_Identity=39.1849529780564, Blast_Score=383, Evalue=1e-107,
Organism=Escherichia coli, GI2367296, Length=702, Percent_Identity=35.3276353276353, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI1787196, Length=349, Percent_Identity=28.6532951289398, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6322369, Length=475, Percent_Identity=30.9473684210526, Blast_Score=159, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6324477, Length=376, Percent_Identity=23.1382978723404, Blast_Score=84, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 80457; Mature: 80326

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVSLSSVQPLVPQTLEQTIVSLNDAQKQAVEHIDGAMLILAGAGSGKTKTITTRLAYLI
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHH
DEVGIPPSATLTLTFTNKAAQEMRERALTLLHNRYESPPLLCTFHRFGLLFLRFHITALG
HHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHCC
RNANFVVLDTDDKRAILKSLCKIRPISQLDYYISDMKNAFLSPEQAQTYAHSEEYKLMNR
CCCCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
IYGEYENYLLNKNLLDFDDLLYLTYAILLKNDNIAKQMSEKYHYIMVDEYQDTNEIQYKI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEEECCCCCHHHHHHH
LKRLCLAHTNLCVVGDDDQSIYGWRGANVKNILGFPKEFDEVKVVKLEENYRSSEQILNA
HHHHHHHCCCEEEECCCCCCEECCCCCCHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHH
ANALISHNSKRLGKNLKSIKGAGANVRVIESENESTEAEFLAKDILTLLSQGINPSEIAV
HHHHHHCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHEEE
LFRVNALSRSIEEGFNRAKIPYKIIGAVRFYERAEIKDLLCYLRLIVNPNDDYSFLRIIN
EEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
RPRRGIGKITQLKLEELARTFNTSCFSSITQYTQQSKEAIGEKAYNALLELTQNIERWRK
CCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YTDLADVIEDMQEHIVFTFSRLDEVDRKGNIEEFYGMFRDYAIANPSNQVEDFLNDIALA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
SPTDELTGEYVSCMSIHMAKGLEFKYVYVIGFEEGIFPLWNDEDEIQEERRLGYVAITRA
CCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHCCCEEEEEHH
KDFLTLCSAKSRLRRGKREWLEQSRFLRESGLLGVYNAPSLYKTQSIQEEAEVVFSKGMK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHCCCH
VQHKLFGVGVVESIKGKGSQCQLRINFSGLERVILASFVQKVCE
HHHHHHCCHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PVSLSSVQPLVPQTLEQTIVSLNDAQKQAVEHIDGAMLILAGAGSGKTKTITTRLAYLI
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHH
DEVGIPPSATLTLTFTNKAAQEMRERALTLLHNRYESPPLLCTFHRFGLLFLRFHITALG
HHCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHCC
RNANFVVLDTDDKRAILKSLCKIRPISQLDYYISDMKNAFLSPEQAQTYAHSEEYKLMNR
CCCCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
IYGEYENYLLNKNLLDFDDLLYLTYAILLKNDNIAKQMSEKYHYIMVDEYQDTNEIQYKI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEEECCCCCHHHHHHH
LKRLCLAHTNLCVVGDDDQSIYGWRGANVKNILGFPKEFDEVKVVKLEENYRSSEQILNA
HHHHHHHCCCEEEECCCCCCEECCCCCCHHHHCCCCCCCCCEEEEEECCCCCHHHHHHHH
ANALISHNSKRLGKNLKSIKGAGANVRVIESENESTEAEFLAKDILTLLSQGINPSEIAV
HHHHHHCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHEEE
LFRVNALSRSIEEGFNRAKIPYKIIGAVRFYERAEIKDLLCYLRLIVNPNDDYSFLRIIN
EEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
RPRRGIGKITQLKLEELARTFNTSCFSSITQYTQQSKEAIGEKAYNALLELTQNIERWRK
CCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YTDLADVIEDMQEHIVFTFSRLDEVDRKGNIEEFYGMFRDYAIANPSNQVEDFLNDIALA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
SPTDELTGEYVSCMSIHMAKGLEFKYVYVIGFEEGIFPLWNDEDEIQEERRLGYVAITRA
CCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCHHHHHHHHCCCEEEEEHH
KDFLTLCSAKSRLRRGKREWLEQSRFLRESGLLGVYNAPSLYKTQSIQEEAEVVFSKGMK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHCCCH
VQHKLFGVGVVESIKGKGSQCQLRINFSGLERVILASFVQKVCE
HHHHHHCCHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA