Click here to switch to the map view.

The map label for this gene is mrdA [H]

Identifier: 32266831

GI number: 32266831

Start: 1287998

End: 1289785

Strand: Direct

Name: mrdA [H]

Synonym: HH1332

Alternate gene names: 32266831

Gene position: 1287998-1289785 (Clockwise)

Preceding gene: 32266830

Following gene: 32266832

Centisome position: 71.59

GC content: 36.63

Gene sequence:

>1788_bases
ATGATTAATTTGCGTTATTGGTTGCTTATTTTCTTTTTTATCGTAGTGTGGTTTATTATTGTAATACGTCTTTTTGTGGT
GAATGTTATTAATCACGAGTATTATGAAAAATTTGCTGAGAAAAATATCACAAAAACTGAAGTGCTTGTGCCAATGCGTG
GGTTAATTTTTGATAGAAATACCGAACCTTTGGCAATTAATGAGATAGGGTTTTCTATCTCACTTATACCCAAAATACGT
AATAGTCGTATTGTCGAATCTGAAATTGAACGAATCATTTCATTTTTGCCCTCACTTGATAAAGAGGAACTTTTCAATCT
CTATAAAAAAAATAATCACGCCTATAACCACTTGCCTATAGAAATTGTAAATTTTGTTCCTTATGCCCAAATGTTGAGTA
TTTATGCCTATCTTAAACGATCTTCTTATGTTGTTATTGCACCCAATAGTAAGCGTCTATATCCAAATGAAAGTGCAGCC
TCACACGTTATAGGCTATGTATCAAAGGCAAATGATAAAGATATACAAAATAATCCTCTTTCTCAATACAGCAAGGTTAT
TGGCAAAGAAGGCATTGAGAAATTTTATAATTCGTATTTACAAGGCGAGGCAGGATATAGAAAATCTCATATTAACTCTC
TTCACAAAGAAATCAAGCTTTTAAAAGAAGATAGCGTGCTTAAACGCAATGATTTATATTTATCGCTTGATTTAAAGCTC
CAAGAAAGTATTGATTCAGAATTTACAGAACGCTCTGGAGCCGTAATTGTGATGAATGTGCATAATGGTGAAATACTTGC
AGCAGGGAGCTATCCTGAATATAATATTAATTGGTTTGTTGATGGCATTAGCTATAAAAATTGGGAGGGACTTATTGAAA
ATGTCCATAAGCCACTTATCAATAAGCTTGTGTATGGGCGCTATCCACCCGGCTCTGTTATTAAAATGGGTATGGTTTTG
GCATTTTTACAATATGCAGGAATCAATGAAAGCACTTTGATTCAAACACCGCCTTTTATAGAATTTGGTGGGCGAAAGTT
TCGCGATTGGAAAGCATCAGGACACGGGAGTGCTGATGCAATCAAAGCAATTAGAGAATCTGTAGATGTGTATTTTTATT
TGCTTTCTCAACGTGCAGGGATTGATAATATGGCAAGTGTATTGCGACAAATGGGCATAGGTGAGCTTACAGGCGTTGAT
ATTCCCAATGAAGTAAGTGGCATACTTCCTACGCCTGAATGGAAATTGCAACGTTATGGTGAGCAATGGTATAAGGGGGA
CACGATTACAACTTCTATTGGGCAAGGGTATTTTCTCTCTACACCTATGCAAATCGCTCGTTATACGGCGCTTATTGCAT
CAGGTAAATTAGTAACACCACATTTTGCAAAAGAATTTAATGAAGAGAGTGCAGAATTTGAGGCTAAAGATATCCTAAGC
GATTTTCAAAAATCTAAACTTGATGTTTTGCGTAAAGGTATGTATCAAGTATGCTCTGTGCCGGGTGGGACAGCATATTA
TCGCACGCAAGGCACAAGAGTAAGTCTTGCGTGTAAAACAGGGACAGCACAAGTAGTGGGTATTCCTCAAGATATACAAA
GACGAACTAGGGAGGCAGATATGGAATATTTTCATCGTTCTCACGCTTGGATTACCGCATTTTTACCCTATGAGAATCCT
CAATATGCAGTTACAATTCTCATTGAACACGGAGGAAGCGGAGGAAGCGGAGGACCTGTGCTTGTAAAAATTGCAAACAA
GCTTAAAGAACTAGGCTATATTAAATAA

Upstream 100 bases:

>100_bases
TGCTATCTTTATTTGATGCTTTTATGGGAAGCACTATCACTCCAAGTATATGGATTGTGCTCTATTATATTGCTATTGAA
ATTCTTGTAGTGATATTCCT

Downstream 100 bases:

>100_bases
TTCAAGGAGGGCTTATGGGACATTTGATTGATTCTAAGAGTATTTTGATTACAGGTGGCACAGGAAGTTTTGGTAAAAAA
TTTGTTGAATATATATTAAA

Product: penicillin-binding protein

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 595; Mature: 595

Protein sequence:

>595_residues
MINLRYWLLIFFFIVVWFIIVIRLFVVNVINHEYYEKFAEKNITKTEVLVPMRGLIFDRNTEPLAINEIGFSISLIPKIR
NSRIVESEIERIISFLPSLDKEELFNLYKKNNHAYNHLPIEIVNFVPYAQMLSIYAYLKRSSYVVIAPNSKRLYPNESAA
SHVIGYVSKANDKDIQNNPLSQYSKVIGKEGIEKFYNSYLQGEAGYRKSHINSLHKEIKLLKEDSVLKRNDLYLSLDLKL
QESIDSEFTERSGAVIVMNVHNGEILAAGSYPEYNINWFVDGISYKNWEGLIENVHKPLINKLVYGRYPPGSVIKMGMVL
AFLQYAGINESTLIQTPPFIEFGGRKFRDWKASGHGSADAIKAIRESVDVYFYLLSQRAGIDNMASVLRQMGIGELTGVD
IPNEVSGILPTPEWKLQRYGEQWYKGDTITTSIGQGYFLSTPMQIARYTALIASGKLVTPHFAKEFNEESAEFEAKDILS
DFQKSKLDVLRKGMYQVCSVPGGTAYYRTQGTRVSLACKTGTAQVVGIPQDIQRRTREADMEYFHRSHAWITAFLPYENP
QYAVTILIEHGGSGGSGGPVLVKIANKLKELGYIK

Sequences:

>Translated_595_residues
MINLRYWLLIFFFIVVWFIIVIRLFVVNVINHEYYEKFAEKNITKTEVLVPMRGLIFDRNTEPLAINEIGFSISLIPKIR
NSRIVESEIERIISFLPSLDKEELFNLYKKNNHAYNHLPIEIVNFVPYAQMLSIYAYLKRSSYVVIAPNSKRLYPNESAA
SHVIGYVSKANDKDIQNNPLSQYSKVIGKEGIEKFYNSYLQGEAGYRKSHINSLHKEIKLLKEDSVLKRNDLYLSLDLKL
QESIDSEFTERSGAVIVMNVHNGEILAAGSYPEYNINWFVDGISYKNWEGLIENVHKPLINKLVYGRYPPGSVIKMGMVL
AFLQYAGINESTLIQTPPFIEFGGRKFRDWKASGHGSADAIKAIRESVDVYFYLLSQRAGIDNMASVLRQMGIGELTGVD
IPNEVSGILPTPEWKLQRYGEQWYKGDTITTSIGQGYFLSTPMQIARYTALIASGKLVTPHFAKEFNEESAEFEAKDILS
DFQKSKLDVLRKGMYQVCSVPGGTAYYRTQGTRVSLACKTGTAQVVGIPQDIQRRTREADMEYFHRSHAWITAFLPYENP
QYAVTILIEHGGSGGSGGPVLVKIANKLKELGYIK
>Mature_595_residues
MINLRYWLLIFFFIVVWFIIVIRLFVVNVINHEYYEKFAEKNITKTEVLVPMRGLIFDRNTEPLAINEIGFSISLIPKIR
NSRIVESEIERIISFLPSLDKEELFNLYKKNNHAYNHLPIEIVNFVPYAQMLSIYAYLKRSSYVVIAPNSKRLYPNESAA
SHVIGYVSKANDKDIQNNPLSQYSKVIGKEGIEKFYNSYLQGEAGYRKSHINSLHKEIKLLKEDSVLKRNDLYLSLDLKL
QESIDSEFTERSGAVIVMNVHNGEILAAGSYPEYNINWFVDGISYKNWEGLIENVHKPLINKLVYGRYPPGSVIKMGMVL
AFLQYAGINESTLIQTPPFIEFGGRKFRDWKASGHGSADAIKAIRESVDVYFYLLSQRAGIDNMASVLRQMGIGELTGVD
IPNEVSGILPTPEWKLQRYGEQWYKGDTITTSIGQGYFLSTPMQIARYTALIASGKLVTPHFAKEFNEESAEFEAKDILS
DFQKSKLDVLRKGMYQVCSVPGGTAYYRTQGTRVSLACKTGTAQVVGIPQDIQRRTREADMEYFHRSHAWITAFLPYENP
QYAVTILIEHGGSGGSGGPVLVKIANKLKELGYIK

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=600, Percent_Identity=31.8333333333333, Blast_Score=325, Evalue=6e-90,
Organism=Escherichia coli, GI1786272, Length=587, Percent_Identity=21.8057921635434, Blast_Score=101, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 67600; Mature: 67600

Theoretical pI: Translated: 8.95; Mature: 8.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINLRYWLLIFFFIVVWFIIVIRLFVVNVINHEYYEKFAEKNITKTEVLVPMRGLIFDRN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEECCHHHEECCC
TEPLAINEIGFSISLIPKIRNSRIVESEIERIISFLPSLDKEELFNLYKKNNHAYNHLPI
CCCEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCH
EIVNFVPYAQMLSIYAYLKRSSYVVIAPNSKRLYPNESAASHVIGYVSKANDKDIQNNPL
HHHHHCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCH
SQYSKVIGKEGIEKFYNSYLQGEAGYRKSHINSLHKEIKLLKEDSVLKRNDLYLSLDLKL
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCEEEEEEEEE
QESIDSEFTERSGAVIVMNVHNGEILAAGSYPEYNINWFVDGISYKNWEGLIENVHKPLI
HHHHHHHHHHCCCCEEEEEECCCCEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHH
NKLVYGRYPPGSVIKMGMVLAFLQYAGINESTLIQTPPFIEFGGRKFRDWKASGHGSADA
HHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCCHHCCCCCCCCCCCCCCCCHHH
IKAIRESVDVYFYLLSQRAGIDNMASVLRQMGIGELTGVDIPNEVSGILPTPEWKLQRYG
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
EQWYKGDTITTSIGQGYFLSTPMQIARYTALIASGKLVTPHFAKEFNEESAEFEAKDILS
HHHHCCCCEEEECCCCEEECCHHHHHHHHHHHHCCCEECCHHHHHCCCHHHCHHHHHHHH
DFQKSKLDVLRKGMYQVCSVPGGTAYYRTQGTRVSLACKTGTAQVVGIPQDIQRRTREAD
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEEECCCEEEEECCHHHHHHHHHHH
MEYFHRSHAWITAFLPYENPQYAVTILIEHGGSGGSGGPVLVKIANKLKELGYIK
HHHHHCCCCEEEEEECCCCCCEEEEEEEEECCCCCCCCCEEHHHHHHHHHCCCCC
>Mature Secondary Structure
MINLRYWLLIFFFIVVWFIIVIRLFVVNVINHEYYEKFAEKNITKTEVLVPMRGLIFDRN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEECCHHHEECCC
TEPLAINEIGFSISLIPKIRNSRIVESEIERIISFLPSLDKEELFNLYKKNNHAYNHLPI
CCCEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCH
EIVNFVPYAQMLSIYAYLKRSSYVVIAPNSKRLYPNESAASHVIGYVSKANDKDIQNNPL
HHHHHCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCH
SQYSKVIGKEGIEKFYNSYLQGEAGYRKSHINSLHKEIKLLKEDSVLKRNDLYLSLDLKL
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEECCEEEEEEEEE
QESIDSEFTERSGAVIVMNVHNGEILAAGSYPEYNINWFVDGISYKNWEGLIENVHKPLI
HHHHHHHHHHCCCCEEEEEECCCCEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHH
NKLVYGRYPPGSVIKMGMVLAFLQYAGINESTLIQTPPFIEFGGRKFRDWKASGHGSADA
HHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCCHHCCCCCCCCCCCCCCCCHHH
IKAIRESVDVYFYLLSQRAGIDNMASVLRQMGIGELTGVDIPNEVSGILPTPEWKLQRYG
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
EQWYKGDTITTSIGQGYFLSTPMQIARYTALIASGKLVTPHFAKEFNEESAEFEAKDILS
HHHHCCCCEEEECCCCEEECCHHHHHHHHHHHHCCCEECCHHHHHCCCHHHCHHHHHHHH
DFQKSKLDVLRKGMYQVCSVPGGTAYYRTQGTRVSLACKTGTAQVVGIPQDIQRRTREAD
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEEECCCEEEEECCHHHHHHHHHHH
MEYFHRSHAWITAFLPYENPQYAVTILIEHGGSGGSGGPVLVKIANKLKELGYIK
HHHHHCCCCEEEEEECCCCCCEEEEEEEEECCCCCCCCCEEHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]