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The map label for this gene is flmA

Identifier: 32266832

GI number: 32266832

Start: 1289800

End: 1290798

Strand: Direct

Name: flmA

Synonym: HH1333

Alternate gene names: 32266832

Gene position: 1289800-1290798 (Clockwise)

Preceding gene: 32266831

Following gene: 32266833

Centisome position: 71.69

GC content: 36.04

Gene sequence:

>999_bases
ATGGGACATTTGATTGATTCTAAGAGTATTTTGATTACAGGTGGCACAGGAAGTTTTGGTAAAAAATTTGTTGAATATAT
ATTAAAATATCATAATCCAAAAAAAATTATTGTTTATAGTCGTGATGAACTAAAGCAATATGAAATGGCGCAGGTTTTTA
ATGACAAACGAATGCGTTATTTCATTGGTGATGTCAGAGATAAAAATCGGCTAGAGAGTGCACTAGATGGCGTGGATATT
TGTATTCACGCGGCTGCACTTAAACACGTGCCTATTGCTGAATACAATCCTATGGAGTGTATAAAAACAAATATTGATGG
TGCGAGTAATGTTATTAGTGCGTGTTTAAGCAATAATGTTGGGCATATTATCGCCCTTAGCACAGATAAGGCTGCAAATC
CTATCAATCTCTATGGGGCGACAAAGTTATGTTCAGATAAGCTTTTTATTGCTGCAAATAATATTAAAGGCAATAAAGAT
TCTAAATTTAGTGTGGTGCGTTACGGGAATGTTGTAGGTTCGCGTGGTTCGGTTATCCCATTTTTTAAAAAACTTATTGA
AGAAGGTGCAAATGAACTGCCTATTACTGATGAGCGTATGAGTAGGTTTTTTATCACTTTAGAATCTGGCGTAAAGTTTG
TGCTTGAGAATTTAGAGCGTATGCACGGAGGCGAGATATTTATACCAAAGATTCCAAGTGTGAAAATTACACAGCTAGCA
AGTGCACTTGCACCACATTTGCCACATAAAATTATCGGCATACGTCCGGGCGAAAAGCTCCACGAAGTAATGGTGCCACG
CGATGATTCTCATTTATGTGCGGAATTTGAACAATTTTTTATTCTTATGCCTACTATTAATTTTCAAACACCTATTGATT
ATTATAAAACGCGTAAGGGAGAAAAAGGAGTTCCTGTGCAATCAGGATTTGAATATAGCAGTGAAACTAATTCGTGGTGG
CTGACACAGAATGAAATTCTCAAAATTATCCAAAAATGA

Upstream 100 bases:

>100_bases
TTGAACACGGAGGAAGCGGAGGAAGCGGAGGACCTGTGCTTGTAAAAATTGCAAACAAGCTTAAAGAACTAGGCTATATT
AAATAATTCAAGGAGGGCTT

Downstream 100 bases:

>100_bases
TAAAATTTAAGCAAGTTGTTAGAAAATACATTTTACAATGCAATGTTTTTAAATTCTCATTAAAGGATACAAAATGATTT
TAGATAGTATTGTAAAAATG

Product: flagellar glycosylation protein FlmA

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MGHLIDSKSILITGGTGSFGKKFVEYILKYHNPKKIIVYSRDELKQYEMAQVFNDKRMRYFIGDVRDKNRLESALDGVDI
CIHAAALKHVPIAEYNPMECIKTNIDGASNVISACLSNNVGHIIALSTDKAANPINLYGATKLCSDKLFIAANNIKGNKD
SKFSVVRYGNVVGSRGSVIPFFKKLIEEGANELPITDERMSRFFITLESGVKFVLENLERMHGGEIFIPKIPSVKITQLA
SALAPHLPHKIIGIRPGEKLHEVMVPRDDSHLCAEFEQFFILMPTINFQTPIDYYKTRKGEKGVPVQSGFEYSSETNSWW
LTQNEILKIIQK

Sequences:

>Translated_332_residues
MGHLIDSKSILITGGTGSFGKKFVEYILKYHNPKKIIVYSRDELKQYEMAQVFNDKRMRYFIGDVRDKNRLESALDGVDI
CIHAAALKHVPIAEYNPMECIKTNIDGASNVISACLSNNVGHIIALSTDKAANPINLYGATKLCSDKLFIAANNIKGNKD
SKFSVVRYGNVVGSRGSVIPFFKKLIEEGANELPITDERMSRFFITLESGVKFVLENLERMHGGEIFIPKIPSVKITQLA
SALAPHLPHKIIGIRPGEKLHEVMVPRDDSHLCAEFEQFFILMPTINFQTPIDYYKTRKGEKGVPVQSGFEYSSETNSWW
LTQNEILKIIQK
>Mature_331_residues
GHLIDSKSILITGGTGSFGKKFVEYILKYHNPKKIIVYSRDELKQYEMAQVFNDKRMRYFIGDVRDKNRLESALDGVDIC
IHAAALKHVPIAEYNPMECIKTNIDGASNVISACLSNNVGHIIALSTDKAANPINLYGATKLCSDKLFIAANNIKGNKDS
KFSVVRYGNVVGSRGSVIPFFKKLIEEGANELPITDERMSRFFITLESGVKFVLENLERMHGGEIFIPKIPSVKITQLAS
ALAPHLPHKIIGIRPGEKLHEVMVPRDDSHLCAEFEQFFILMPTINFQTPIDYYKTRKGEKGVPVQSGFEYSSETNSWWL
TQNEILKIIQK

Specific function: Galactose metabolism; third step. [C]

COG id: COG1086

COG function: function code MG; Predicted nucleoside-diphosphate sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR003869 [H]

Pfam domain/function: PF02719 Polysacc_synt_2 [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 37341; Mature: 37209

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGHLIDSKSILITGGTGSFGKKFVEYILKYHNPKKIIVYSRDELKQYEMAQVFNDKRMRY
CCCEECCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHCCCCEEE
FIGDVRDKNRLESALDGVDICIHAAALKHVPIAEYNPMECIKTNIDGASNVISACLSNNV
EECCCCCHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCHHHHHHHHHCCCC
GHIIALSTDKAANPINLYGATKLCSDKLFIAANNIKGNKDSKFSVVRYGNVVGSRGSVIP
CEEEEEECCCCCCCEEECCCHHHCCCCEEEEECCCCCCCCCCEEEEEECCEECCCCCCHH
FFKKLIEEGANELPITDERMSRFFITLESGVKFVLENLERMHGGEIFIPKIPSVKITQLA
HHHHHHHCCCCCCCCCHHHHHEEEEEHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHH
SALAPHLPHKIIGIRPGEKLHEVMVPRDDSHLCAEFEQFFILMPTINFQTPIDYYKTRKG
HHHHHCCCCCEEECCCCHHHHHHCCCCCCCHHHHHHHCEEEEEECCCCCCCHHHHHCCCC
EKGVPVQSGFEYSSETNSWWLTQNEILKIIQK
CCCCCCCCCCCCCCCCCCEEECHHHHHHHHCC
>Mature Secondary Structure 
GHLIDSKSILITGGTGSFGKKFVEYILKYHNPKKIIVYSRDELKQYEMAQVFNDKRMRY
CCEECCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHCCCCEEE
FIGDVRDKNRLESALDGVDICIHAAALKHVPIAEYNPMECIKTNIDGASNVISACLSNNV
EECCCCCHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCHHHHHHHHHCCCC
GHIIALSTDKAANPINLYGATKLCSDKLFIAANNIKGNKDSKFSVVRYGNVVGSRGSVIP
CEEEEEECCCCCCCEEECCCHHHCCCCEEEEECCCCCCCCCCEEEEEECCEECCCCCCHH
FFKKLIEEGANELPITDERMSRFFITLESGVKFVLENLERMHGGEIFIPKIPSVKITQLA
HHHHHHHCCCCCCCCCHHHHHEEEEEHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHH
SALAPHLPHKIIGIRPGEKLHEVMVPRDDSHLCAEFEQFFILMPTINFQTPIDYYKTRKG
HHHHHCCCCCEEECCCCHHHHHHCCCCCCCHHHHHHHCEEEEEECCCCCCCHHHHHCCCC
EKGVPVQSGFEYSSETNSWWLTQNEILKIIQK
CCCCCCCCCCCCCCCCCCEEECHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]