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The map label for this gene is dniR

Identifier: 32266822

GI number: 32266822

Start: 1282265

End: 1283389

Strand: Direct

Name: dniR

Synonym: HH1323

Alternate gene names: 32266822

Gene position: 1282265-1283389 (Clockwise)

Preceding gene: 32266821

Following gene: 32266823

Centisome position: 71.27

GC content: 36.44

Gene sequence:

>1125_bases
ATGAAAAATGCAAAGTGTCATATATGTTTGATAATTTTTGCTTTATTTTGTGTAGTAAATGTGTCATTGAGCGATGAAAA
TGGTTTTTATACAATGACACATAATAGAAGTGCAAATAATGTGCTTAATTCATTCGGGGTACATACAGCTTTTATTGCGA
CTATCGCAGATGATGCAAGAGCACAGAAAATACAAGAGAAATGGAAGTATTTTGTGCAACGTTTTGAGCATAGTTATGAA
TTTATCCCTACATTAAAAAATATGATGGCGCGAGAGCAGATTCCTCAAGAGTTTTTATTTCTTGCTATGGCAGAATCCGA
ATTTGCCCCGAGTGCAAAAAGCTCTAAAAAAGCCATAGGTATTTGGCAGATTATGCCAGCAACGGGTAAAAGTTTAGGCT
TAGAAATTAATTCATATATTGATGAGCGTAGAGATCCGATTAAAAGCACAGAGGCAGCAATAAAGTATCTTAAATATCTT
TATGATGCCACAGGTGAATGGTATTTGGCAGCAATGGCATATAATTGTGGGCTTGGACGCTTAAAGCGAGGCATTGAAGA
GGCAGGAGGCGATAAGCGCATTGAAACGCTGCTTGATGATGAGGCTCAATATATTCCAGCTGAAACACGCAATTATATTC
GCACAATTTTGGCTATGAGTTTGCTTTTTAATGATGTAGATTTTCTTAAAGCGCAAAATGCTGATTATTTACTTAATCGT
GGGGCAACAGATAGTATTACAAGTGTGAGCATTAGGGGTGGAACATCATTAAGTGCGATTGCAAAAAGTGCGAGGATTCC
ATTAGGTGAATTACAAAAGTATAATCGTCATTTTACGCGTTCTTTGCTTCCACAGGGTAATAAGCGCTATAATGTCTATA
TTCCTTATGATAAGCTGCGCACTTTTAAGAGATATTTTAATGAAAAAAGCTATCCTCAAGCTTCATTTATAACCCATATT
GTGCAAAAGGGCGAGAATCTAGGTTCTATTGCACGGAATTATAAAATTTCGCTCAATCAACTACAAGCGGCAAATAATAT
CAAAGGTTCACATATCGCAATTAATCAAAAGCTCATAATTCCTGTGCTCAAAGGCAAATCTACGACGATTGCCGATAATA
GGTAG

Upstream 100 bases:

>100_bases
AAGTGCTTGGTATGAGTGAAGAGGAAGTGATAGATATAAGCACACACAACGCTATTACGCTTTTTAAAGAGATTCAATAA
AGAAAGAGGAAGGAATGCCA

Downstream 100 bases:

>100_bases
AGTAAAGGAATAGAATGAAAGGTCTAAAAAAAATACAAATTGCTATAAGTTTAAGTGTGTTGTGTGGGCTGTTTTGGGGT
TGTTTAGGAAAAGATACACA

Product: hypothetical protein

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MKNAKCHICLIIFALFCVVNVSLSDENGFYTMTHNRSANNVLNSFGVHTAFIATIADDARAQKIQEKWKYFVQRFEHSYE
FIPTLKNMMAREQIPQEFLFLAMAESEFAPSAKSSKKAIGIWQIMPATGKSLGLEINSYIDERRDPIKSTEAAIKYLKYL
YDATGEWYLAAMAYNCGLGRLKRGIEEAGGDKRIETLLDDEAQYIPAETRNYIRTILAMSLLFNDVDFLKAQNADYLLNR
GATDSITSVSIRGGTSLSAIAKSARIPLGELQKYNRHFTRSLLPQGNKRYNVYIPYDKLRTFKRYFNEKSYPQASFITHI
VQKGENLGSIARNYKISLNQLQAANNIKGSHIAINQKLIIPVLKGKSTTIADNR

Sequences:

>Translated_374_residues
MKNAKCHICLIIFALFCVVNVSLSDENGFYTMTHNRSANNVLNSFGVHTAFIATIADDARAQKIQEKWKYFVQRFEHSYE
FIPTLKNMMAREQIPQEFLFLAMAESEFAPSAKSSKKAIGIWQIMPATGKSLGLEINSYIDERRDPIKSTEAAIKYLKYL
YDATGEWYLAAMAYNCGLGRLKRGIEEAGGDKRIETLLDDEAQYIPAETRNYIRTILAMSLLFNDVDFLKAQNADYLLNR
GATDSITSVSIRGGTSLSAIAKSARIPLGELQKYNRHFTRSLLPQGNKRYNVYIPYDKLRTFKRYFNEKSYPQASFITHI
VQKGENLGSIARNYKISLNQLQAANNIKGSHIAINQKLIIPVLKGKSTTIADNR
>Mature_374_residues
MKNAKCHICLIIFALFCVVNVSLSDENGFYTMTHNRSANNVLNSFGVHTAFIATIADDARAQKIQEKWKYFVQRFEHSYE
FIPTLKNMMAREQIPQEFLFLAMAESEFAPSAKSSKKAIGIWQIMPATGKSLGLEINSYIDERRDPIKSTEAAIKYLKYL
YDATGEWYLAAMAYNCGLGRLKRGIEEAGGDKRIETLLDDEAQYIPAETRNYIRTILAMSLLFNDVDFLKAQNADYLLNR
GATDSITSVSIRGGTSLSAIAKSARIPLGELQKYNRHFTRSLLPQGNKRYNVYIPYDKLRTFKRYFNEKSYPQASFITHI
VQKGENLGSIARNYKISLNQLQAANNIKGSHIAINQKLIIPVLKGKSTTIADNR

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=288, Percent_Identity=29.8611111111111, Blast_Score=108, Evalue=6e-25,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 42363; Mature: 42363

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNAKCHICLIIFALFCVVNVSLSDENGFYTMTHNRSANNVLNSFGVHTAFIATIADDAR
CCCCCHHHHHHHHHHHHHHCCEEECCCCEEEEECCCCHHHHHHHHCHHHHHHHHHHHHHH
AQKIQEKWKYFVQRFEHSYEFIPTLKNMMAREQIPQEFLFLAMAESEFAPSAKSSKKAIG
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCEEE
IWQIMPATGKSLGLEINSYIDERRDPIKSTEAAIKYLKYLYDATGEWYLAAMAYNCGLGR
EEEECCCCCCCCCEEHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCHHH
LKRGIEEAGGDKRIETLLDDEAQYIPAETRNYIRTILAMSLLFNDVDFLKAQNADYLLNR
HHHHHHHCCCCHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHCC
GATDSITSVSIRGGTSLSAIAKSARIPLGELQKYNRHFTRSLLPQGNKRYNVYIPYDKLR
CCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHH
TFKRYFNEKSYPQASFITHIVQKGENLGSIARNYKISLNQLQAANNIKGSHIAINQKLII
HHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHEEHHHHHHHCCCCCCEEEECCEEEE
PVLKGKSTTIADNR
EEECCCCCCCCCCC
>Mature Secondary Structure
MKNAKCHICLIIFALFCVVNVSLSDENGFYTMTHNRSANNVLNSFGVHTAFIATIADDAR
CCCCCHHHHHHHHHHHHHHCCEEECCCCEEEEECCCCHHHHHHHHCHHHHHHHHHHHHHH
AQKIQEKWKYFVQRFEHSYEFIPTLKNMMAREQIPQEFLFLAMAESEFAPSAKSSKKAIG
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCEEE
IWQIMPATGKSLGLEINSYIDERRDPIKSTEAAIKYLKYLYDATGEWYLAAMAYNCGLGR
EEEECCCCCCCCCEEHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCHHH
LKRGIEEAGGDKRIETLLDDEAQYIPAETRNYIRTILAMSLLFNDVDFLKAQNADYLLNR
HHHHHHHCCCCHHHHHHHCCCHHCCCHHHHHHHHHHHHHHHHHCCHHHHHCCCCCHHHCC
GATDSITSVSIRGGTSLSAIAKSARIPLGELQKYNRHFTRSLLPQGNKRYNVYIPYDKLR
CCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECHHHHH
TFKRYFNEKSYPQASFITHIVQKGENLGSIARNYKISLNQLQAANNIKGSHIAINQKLII
HHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHEEHHHHHHHCCCCCCEEEECCEEEE
PVLKGKSTTIADNR
EEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]