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The map label for this gene is tilS

Identifier: 32266754

GI number: 32266754

Start: 1218550

End: 1219821

Strand: Direct

Name: tilS

Synonym: HH1255

Alternate gene names: 32266754

Gene position: 1218550-1219821 (Clockwise)

Preceding gene: 32266753

Following gene: 32266755

Centisome position: 67.73

GC content: 34.43

Gene sequence:

>1272_bases
GTGGCTAAAAAAGATGCTTATAAGCTGACATATAATACTTCTGATACTTTATTTGCAACGAATCTTGGAGATATTCTTGC
CCCCTCTTTTGCCTCAATTAAACAAACACGTAATTTGCTTGGTTTCTCTAGTGGTGTAGATTCTACGGCACTTTTTTTTC
TCTTACTTGAGTGTGAGATTCCCTTTGATATTGCCATTGTGCATTATCATACGCGCTTACAAGCTGATGATGAAGTTGCT
TATGCTAAAGAGCTTGCCGCTACTTATAATAAGCTTTGTTTTGTGGCACACGCACCACATTTTAATGCAAATTTTGAAAG
CAATGCACGCTCTTTTCGCTTTGATTTTTTTCAGTCTTTAATTATTAAACACCACTACACACATCTTTTGCTAGCTCATC
AACTTAATGATCGCTTAGAATGGTTACTTATGCAGCTTACAAAAGGTGCAGGACTTGGGAATTTGCTAGGTTTTGCAGAC
GAACGATATAACTATGATAAATCTCTAAATAATTATACAATCGTGCGCCCTTTAGAATCTATACCTAAAAATGAATTGTA
TAGATTCTGTAAGGAGCGTGGAATAAAGTATTTTGAAGATAGTAGCAATCAAGATAAGACATTTAGGCGAAATTATTTTA
GATATGAATTTTGCGATAAATTAGTGAATGAGTTTAGCGCAGGCATTGCACGCAGTTTATCCTATCTCCAACGTGATAGG
CAAAGCCTTGAAAATATGCTCTATGCAGATACTTTAGAGCTAGAATCTTTGAAGATGCAGATTCTAAGACAAATACATAA
TATTAGTGTTCCAATGCACACCCATAAAATATGCTGTATAATTTTTAGTATTCATAAATCAAAGGTGCAAGATGATGAAA
ATTTACTTTTGCTTTTTTGCGATAAGGTAGCCAAACAATGTGGATATGTGCTTAGCGCAGCTCAAAGAGATGAGATAAGC
AAAAGCAGATTTAATTGCAAAATTGCTCATTTTATTATTACAAGCAATACTCATAGAATCTATATTGCACAAGATTCTAT
GAGTATGTATAAAATAGTAACACAAAGCCCTATGTCAAAAAAATTTAAAATATTTTGTGCATCCCATCGTGTGCCCTCAA
AGCTTCGGTTCTTGCTGTGGGCGGAATTTTGCGCTTGGGAAATGTTATGTAAAAGTGCATATGCAGATGCTGATTATTTG
GTAAATGAGCAAGGAAACCAACATCTTTTCACTCATTTTTTAGCAAAAATAGACAACTTTTTTACCCTTTAG

Upstream 100 bases:

>100_bases
TCCACATTCTAAAATAAGCCCACATACAAATTCACATAGTTCTTAATTTCTTGTTTTGGATTGTGTTATTATTACATTAA
TTCATAAACAAAGGTAAAGT

Downstream 100 bases:

>100_bases
AAAAGCTTTGTTGTTTTTTTATTAACTTTTTAAGGCAGCTATAACTTCAAAGGCACTATAATCAAATTTGTATTTTAATA
ACTTTCAAAGGACAAGGAGA

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MAKKDAYKLTYNTSDTLFATNLGDILAPSFASIKQTRNLLGFSSGVDSTALFFLLLECEIPFDIAIVHYHTRLQADDEVA
YAKELAATYNKLCFVAHAPHFNANFESNARSFRFDFFQSLIIKHHYTHLLLAHQLNDRLEWLLMQLTKGAGLGNLLGFAD
ERYNYDKSLNNYTIVRPLESIPKNELYRFCKERGIKYFEDSSNQDKTFRRNYFRYEFCDKLVNEFSAGIARSLSYLQRDR
QSLENMLYADTLELESLKMQILRQIHNISVPMHTHKICCIIFSIHKSKVQDDENLLLLFCDKVAKQCGYVLSAAQRDEIS
KSRFNCKIAHFIITSNTHRIYIAQDSMSMYKIVTQSPMSKKFKIFCASHRVPSKLRFLLWAEFCAWEMLCKSAYADADYL
VNEQGNQHLFTHFLAKIDNFFTL

Sequences:

>Translated_423_residues
MAKKDAYKLTYNTSDTLFATNLGDILAPSFASIKQTRNLLGFSSGVDSTALFFLLLECEIPFDIAIVHYHTRLQADDEVA
YAKELAATYNKLCFVAHAPHFNANFESNARSFRFDFFQSLIIKHHYTHLLLAHQLNDRLEWLLMQLTKGAGLGNLLGFAD
ERYNYDKSLNNYTIVRPLESIPKNELYRFCKERGIKYFEDSSNQDKTFRRNYFRYEFCDKLVNEFSAGIARSLSYLQRDR
QSLENMLYADTLELESLKMQILRQIHNISVPMHTHKICCIIFSIHKSKVQDDENLLLLFCDKVAKQCGYVLSAAQRDEIS
KSRFNCKIAHFIITSNTHRIYIAQDSMSMYKIVTQSPMSKKFKIFCASHRVPSKLRFLLWAEFCAWEMLCKSAYADADYL
VNEQGNQHLFTHFLAKIDNFFTL
>Mature_422_residues
AKKDAYKLTYNTSDTLFATNLGDILAPSFASIKQTRNLLGFSSGVDSTALFFLLLECEIPFDIAIVHYHTRLQADDEVAY
AKELAATYNKLCFVAHAPHFNANFESNARSFRFDFFQSLIIKHHYTHLLLAHQLNDRLEWLLMQLTKGAGLGNLLGFADE
RYNYDKSLNNYTIVRPLESIPKNELYRFCKERGIKYFEDSSNQDKTFRRNYFRYEFCDKLVNEFSAGIARSLSYLQRDRQ
SLENMLYADTLELESLKMQILRQIHNISVPMHTHKICCIIFSIHKSKVQDDENLLLLFCDKVAKQCGYVLSAAQRDEISK
SRFNCKIAHFIITSNTHRIYIAQDSMSMYKIVTQSPMSKKFKIFCASHRVPSKLRFLLWAEFCAWEMLCKSAYADADYLV
NEQGNQHLFTHFLAKIDNFFTL

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family

Homologues:

Organism=Escherichia coli, GI1786386, Length=205, Percent_Identity=25.8536585365854, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TILS_HELHP (Q7VGR5)

Other databases:

- EMBL:   AE017125
- RefSeq:   NP_860786.1
- ProteinModelPortal:   Q7VGR5
- SMR:   Q7VGR5
- GeneID:   1492943
- GenomeReviews:   AE017125_GR
- KEGG:   hhe:HH1255
- NMPDR:   fig|235279.1.peg.1255
- HOGENOM:   HBG321119
- ProtClustDB:   CLSK872424
- BioCyc:   HHEP235279:HH_1255-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_01161
- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11807:SF2
- TIGRFAMs:   TIGR02432

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 49218; Mature: 49087

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKDAYKLTYNTSDTLFATNLGDILAPSFASIKQTRNLLGFSSGVDSTALFFLLLECEI
CCCCCCEEEEECCCCCEEEECCHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHEEECC
PFDIAIVHYHTRLQADDEVAYAKELAATYNKLCFVAHAPHFNANFESNARSFRFDFFQSL
CCEEEEEEEEECCCCCCHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCHHHHHHHHHHH
IIKHHYTHLLLAHQLNDRLEWLLMQLTKGAGLGNLLGFADERYNYDKSLNNYTIVRPLES
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCEEEECCHHH
IPKNELYRFCKERGIKYFEDSSNQDKTFRRNYFRYEFCDKLVNEFSAGIARSLSYLQRDR
CCHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSLENMLYADTLELESLKMQILRQIHNISVPMHTHKICCIIFSIHKSKVQDDENLLLLFC
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHEEHHHHHHCCCCCCEEEEEH
DKVAKQCGYVLSAAQRDEISKSRFNCKIAHFIITSNTHRIYIAQDSMSMYKIVTQSPMSK
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCEEEEEECCCHHHHHHHCCCCCC
KFKIFCASHRVPSKLRFLLWAEFCAWEMLCKSAYADADYLVNEQGNQHLFTHFLAKIDNF
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHH
FTL
CCC
>Mature Secondary Structure 
AKKDAYKLTYNTSDTLFATNLGDILAPSFASIKQTRNLLGFSSGVDSTALFFLLLECEI
CCCCCEEEEECCCCCEEEECCHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHEEECC
PFDIAIVHYHTRLQADDEVAYAKELAATYNKLCFVAHAPHFNANFESNARSFRFDFFQSL
CCEEEEEEEEECCCCCCHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCHHHHHHHHHHH
IIKHHYTHLLLAHQLNDRLEWLLMQLTKGAGLGNLLGFADERYNYDKSLNNYTIVRPLES
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCEEEECCHHH
IPKNELYRFCKERGIKYFEDSSNQDKTFRRNYFRYEFCDKLVNEFSAGIARSLSYLQRDR
CCHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSLENMLYADTLELESLKMQILRQIHNISVPMHTHKICCIIFSIHKSKVQDDENLLLLFC
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHEEHHHHHHCCCCCCEEEEEH
DKVAKQCGYVLSAAQRDEISKSRFNCKIAHFIITSNTHRIYIAQDSMSMYKIVTQSPMSK
HHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCEEEEEECCCHHHHHHHCCCCCC
KFKIFCASHRVPSKLRFLLWAEFCAWEMLCKSAYADADYLVNEQGNQHLFTHFLAKIDNF
CEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHH
FTL
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12810954