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The map label for this gene is miaA

Identifier: 32266409

GI number: 32266409

Start: 883938

End: 884846

Strand: Direct

Name: miaA

Synonym: HH0910

Alternate gene names: 32266409

Gene position: 883938-884846 (Clockwise)

Preceding gene: 32266408

Following gene: 32266413

Centisome position: 49.13

GC content: 37.73

Gene sequence:

>909_bases
ATGAAAATCATTGCAGTGCTTGGCTCAAGTGGAAGCGGCAAGAGTGCTCTTGCTCATCGCATAGCAATGGAGCAAAATTG
TAAGATTTTTAGCCTTGATTCTCTAAGTATTTATAAATATCTTGATATTGCTTCTGCTAAACCCACACTCTTGGAGCAAT
CACAGGTATGTTATTATGCGCTTAATATCCTAGAGCCACATCAAAAAAGCAATGTGATGATTTTTAAAGATCTTCTGCTC
CAAAGCATTGAAGATATCAAAAATAACTCTCCACACACGCCTTTGCTTATTGTAGGCGGAAGTAGCTTTTTTCTTAAAAG
CATTATGGAGGGCTTAAGCCCTATGCCTCCCCTTGAAGAACACGAAGAGTGGGTAAAAAGTCTAGGAAATATAAGTATGC
AATACGCACAATTAACACAAATTGATAAAACTTATGCTCAAAGTCTCTCACCCACAGATACTTACAGAATCTGCAAAGCC
CTTGCGCTCTTTAAAGCCACAAATACACCTCCAAGTATATATTTTGCTACACATAAAAAAGAGTCTTTAGGCTATGACAT
TGAGATTTTTTGCCTTGAATGCGAACGCGATGAGTTAAGAGAGCGCATTGCTAAACGCACAAAAGCAATGATTCAAAAAG
GGATTGTGGAGGAAGTGCAAAATGTATTAGAAGCTTATGGCGCACAAGCTCCTGCGTTAAACGCTATTGGCGCAAAAGAA
TGTGTAAATTTTTTGCAAGGCAAAGTTGCGACTTTACAACAGCTTGAAGAACAAATCTTTTTTCATACTTGCCAACTTGC
CAAACGGCAGCGCACCTTTAATCGCACTCAATTTGCACAAATTACACATTTAAAGGAAAAAGCATTAGAGGCACAATTAA
TACAGCAAATTCATAATAATATTCTTTGA

Upstream 100 bases:

>100_bases
CTAGAACGATTAAATCTTAGGAAATTTGATGGCACAGGAAATAAAGTATTTCTCACTACCTATTATGAGATATTAGATAA
ATAGGTCTCTAACTTCATAT

Downstream 100 bases:

>100_bases
TTATTCCTTTTTGCCATATCGTTCAAAGTAGGTTTTCTCAAGCTCATACAACTCATATCTTACCTTTTTAAAATCAGCAC
TATTGCCAAAAGTAGGAACA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MKIIAVLGSSGSGKSALAHRIAMEQNCKIFSLDSLSIYKYLDIASAKPTLLEQSQVCYYALNILEPHQKSNVMIFKDLLL
QSIEDIKNNSPHTPLLIVGGSSFFLKSIMEGLSPMPPLEEHEEWVKSLGNISMQYAQLTQIDKTYAQSLSPTDTYRICKA
LALFKATNTPPSIYFATHKKESLGYDIEIFCLECERDELRERIAKRTKAMIQKGIVEEVQNVLEAYGAQAPALNAIGAKE
CVNFLQGKVATLQQLEEQIFFHTCQLAKRQRTFNRTQFAQITHLKEKALEAQLIQQIHNNIL

Sequences:

>Translated_302_residues
MKIIAVLGSSGSGKSALAHRIAMEQNCKIFSLDSLSIYKYLDIASAKPTLLEQSQVCYYALNILEPHQKSNVMIFKDLLL
QSIEDIKNNSPHTPLLIVGGSSFFLKSIMEGLSPMPPLEEHEEWVKSLGNISMQYAQLTQIDKTYAQSLSPTDTYRICKA
LALFKATNTPPSIYFATHKKESLGYDIEIFCLECERDELRERIAKRTKAMIQKGIVEEVQNVLEAYGAQAPALNAIGAKE
CVNFLQGKVATLQQLEEQIFFHTCQLAKRQRTFNRTQFAQITHLKEKALEAQLIQQIHNNIL
>Mature_302_residues
MKIIAVLGSSGSGKSALAHRIAMEQNCKIFSLDSLSIYKYLDIASAKPTLLEQSQVCYYALNILEPHQKSNVMIFKDLLL
QSIEDIKNNSPHTPLLIVGGSSFFLKSIMEGLSPMPPLEEHEEWVKSLGNISMQYAQLTQIDKTYAQSLSPTDTYRICKA
LALFKATNTPPSIYFATHKKESLGYDIEIFCLECERDELRERIAKRTKAMIQKGIVEEVQNVLEAYGAQAPALNAIGAKE
CVNFLQGKVATLQQLEEQIFFHTCQLAKRQRTFNRTQFAQITHLKEKALEAQLIQQIHNNIL

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=258, Percent_Identity=24.4186046511628, Blast_Score=76, Evalue=5e-14,
Organism=Escherichia coli, GI1790613, Length=286, Percent_Identity=29.3706293706294, Blast_Score=105, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI25144712, Length=298, Percent_Identity=26.8456375838926, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324848, Length=285, Percent_Identity=24.5614035087719, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24649830, Length=284, Percent_Identity=27.4647887323944, Blast_Score=103, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_HELHP (Q7U323)

Other databases:

- EMBL:   AE017125
- RefSeq:   NP_860441.1
- GeneID:   1492599
- GenomeReviews:   AE017125_GR
- KEGG:   hhe:HH0910
- NMPDR:   fig|235279.1.peg.910
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- BioCyc:   HHEP235279:HH_0910-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 34055; Mature: 34055

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIIAVLGSSGSGKSALAHRIAMEQNCKIFSLDSLSIYKYLDIASAKPTLLEQSQVCYYA
CEEEEEECCCCCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHH
LNILEPHQKSNVMIFKDLLLQSIEDIKNNSPHTPLLIVGGSSFFLKSIMEGLSPMPPLEE
HHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHCCCCCCHHH
HEEWVKSLGNISMQYAQLTQIDKTYAQSLSPTDTYRICKALALFKATNTPPSIYFATHKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCC
ESLGYDIEIFCLECERDELRERIAKRTKAMIQKGIVEEVQNVLEAYGAQAPALNAIGAKE
CCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCHHH
CVNFLQGKVATLQQLEEQIFFHTCQLAKRQRTFNRTQFAQITHLKEKALEAQLIQQIHNN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
IL
CC
>Mature Secondary Structure
MKIIAVLGSSGSGKSALAHRIAMEQNCKIFSLDSLSIYKYLDIASAKPTLLEQSQVCYYA
CEEEEEECCCCCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHCCCCCHHHHHHHHHHHH
LNILEPHQKSNVMIFKDLLLQSIEDIKNNSPHTPLLIVGGSSFFLKSIMEGLSPMPPLEE
HHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHCCCCCCHHH
HEEWVKSLGNISMQYAQLTQIDKTYAQSLSPTDTYRICKALALFKATNTPPSIYFATHKK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCC
ESLGYDIEIFCLECERDELRERIAKRTKAMIQKGIVEEVQNVLEAYGAQAPALNAIGAKE
CCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCHHH
CVNFLQGKVATLQQLEEQIFFHTCQLAKRQRTFNRTQFAQITHLKEKALEAQLIQQIHNN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
IL
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12810954