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The map label for this gene is hup [H]

Identifier: 32266292

GI number: 32266292

Start: 768908

End: 769195

Strand: Direct

Name: hup [H]

Synonym: HH0793

Alternate gene names: 32266292

Gene position: 768908-769195 (Clockwise)

Preceding gene: 32266291

Following gene: 32266295

Centisome position: 42.74

GC content: 34.72

Gene sequence:

>288_bases
ATGAACAAGGCTGAGTTTGTAGATTTGGTTAAGAAAGTTGGTGAATACAACACAAAAAAAGATGCTGAACAAGCAGTAAA
TTCTTTTGTAGATGCTATCTCCAAAGCTCTTTCTAAAAAGCAAAGTGTAGAACTTGTAGGTTTTGGTAAATTTGAAACAG
CTATTCAAAAGGCTAAAACAGGTAAAGTTCCCGGGACAAACAAAACTTACACAACAAAAGAAAAATCTGTGCCTAAGTTC
CGCCCTGGAAAAGGTTTGAAAGACCAAGTAGCAAAAGCTAAAAAATAA

Upstream 100 bases:

>100_bases
TTTATAATAAATTTATGCAAAATATCCGCATATACCTTGACTTAAGACTTAAAATTGTATAGAATGTGAGCGTTCAAAAT
TTAACATCAAGGAGTTTGTA

Downstream 100 bases:

>100_bases
GTCTTTCTTTGCATAGCCTTTTTGGCTATGCCATTAGTGCTTTTGAACTTTCTCTTACATTTTTTCCATTTATAATCAGA
TTAATCTCTCTCGCAATAGG

Product: histone-like DNA-binding protein HU (HupB)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 95; Mature: 95

Protein sequence:

>95_residues
MNKAEFVDLVKKVGEYNTKKDAEQAVNSFVDAISKALSKKQSVELVGFGKFETAIQKAKTGKVPGTNKTYTTKEKSVPKF
RPGKGLKDQVAKAKK

Sequences:

>Translated_95_residues
MNKAEFVDLVKKVGEYNTKKDAEQAVNSFVDAISKALSKKQSVELVGFGKFETAIQKAKTGKVPGTNKTYTTKEKSVPKF
RPGKGLKDQVAKAKK
>Mature_95_residues
MNKAEFVDLVKKVGEYNTKKDAEQAVNSFVDAISKALSKKQSVELVGFGKFETAIQKAKTGKVPGTNKTYTTKEKSVPKF
RPGKGLKDQVAKAKK

Specific function: Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions [H]

COG id: COG0776

COG function: function code L; Bacterial nucleoid DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial histone-like protein family [H]

Homologues:

Organism=Escherichia coli, GI1786644, Length=90, Percent_Identity=36.6666666666667, Blast_Score=62, Evalue=8e-12,
Organism=Escherichia coli, GI1790433, Length=90, Percent_Identity=36.6666666666667, Blast_Score=59, Evalue=5e-11,

Paralogues:

None

Copy number: 860 Molecules/Cell In: Growth-Phase, Minimal Media (Based on E. coli). 2040 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000119
- InterPro:   IPR010992 [H]

Pfam domain/function: PF00216 Bac_DNA_binding [H]

EC number: NA

Molecular weight: Translated: 10407; Mature: 10407

Theoretical pI: Translated: 10.71; Mature: 10.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKAEFVDLVKKVGEYNTKKDAEQAVNSFVDAISKALSKKQSVELVGFGKFETAIQKAKT
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHC
GKVPGTNKTYTTKEKSVPKFRPGKGLKDQVAKAKK
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCC
>Mature Secondary Structure
MNKAEFVDLVKKVGEYNTKKDAEQAVNSFVDAISKALSKKQSVELVGFGKFETAIQKAKT
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHC
GKVPGTNKTYTTKEKSVPKFRPGKGLKDQVAKAKK
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]