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The map label for this gene is gatB

Identifier: 32266295

GI number: 32266295

Start: 771081

End: 772505

Strand: Direct

Name: gatB

Synonym: HH0796

Alternate gene names: 32266295

Gene position: 771081-772505 (Clockwise)

Preceding gene: 32266292

Following gene: 32266296

Centisome position: 42.86

GC content: 40.98

Gene sequence:

>1425_bases
ATGTCAGCGTATGAAACGATTATCGGTTTAGAGGTGCATGTCCAACTCAATACCAAAACAAAAATTTTTTGCTTTTGCGC
AACAAGCTTTGGTGATGAGCCTAACAAAAATGTTTGCCCCACTTGCTTAGGGCTACCCGGCGCACTCCCCGTGCTTAATC
GCGAAGCGGTAAAAAAGGCGATTTCCTTTGGCACTGCGATTAATGCGACTATTAATCAAAACTCTGTCTTTGCACGCAAA
AACTATTTTTACCCAGACTTGCCAAAAGCCTATCAAATCAGTCAATTTGAGATTCCTATCGTAGGACGAGGAAGTATTGA
AATTGAATGTAATAACCAAACAAAAACTATCGGTGTAACGCGTGCGCACCTTGAAGAAGATGCAGGTAAAAATATCCACG
AAAATAATTATTCAAAAGTAGATTTAAATCGTGCCTGTACGCCTCTACTAGAAATTGTGAGTGAGCCCGATATGCGAAGT
TCCGATGAGGCAATAGCCTATCTTAAAAAGCTGCATTCTATTGTGCGATTTCTTGGCATAAGCGATGCAAATATGCAAGA
AGGAAGCTTTAGATGTGATGCAAACGTTTCTATTCGCCCCAAAGGAGATTGCAAACTCTATACGCGTGTAGAGATTAAGA
ATCTTAACTCCTTTAAATTTATCCAAAAAGCGATTGAATATGAGGTAGAGCGACAAATTGAAGCTTGGGAAGAGGGAAAA
TATCAAAGCGAAGTTGTGCAAGAAACTCGTCTTTTTGACACTGCCAAAGGCATTACTCGTTCAATGCGTGGCAAGGAAGA
GGCGGCTGATTATCGCTATTTCCCTGACCCTGACCTTTTGCCTGTTTTTATTGATGAGAATCTTATGCGTGAGGGAGTGA
AGATTCCAGAAATGCCCGATGAAAAAAGGGAGCGATACATTAATACCTTAGGACTTAAGCCTTATGATGCAGGTGTGCTT
ACAAGTAGCCTTGAGCTTGCGCTTTATTTTGAATCTATGCTTGAAGAAGGTGCGAGCGCAAAAGGTGCTCTTACTTGGCT
TACTACAGAGCTTTTAGGACGATTAAAGGGTGAAAATACGCTGCAGACTTGTGGGGTGGATTCTAAAACTTTAGCCACAC
TTGTTAAACGCATTGAGGAGGGCAAAATCAGCGGAAAAAGTGGCAAAGAAATTTTAGATGTGCTTATGGAAAAAGGAGGC
GATGTAGATAGCCTCATAGATTCTATGGGATTAGCCCAAATTAATGATGATGGCGCAATTATTGCAGTTATAGAATCTGT
GTTAAGCGCTAATGCCGACAAGGTCGCGGAATACAAAAGTGGCAAAGACAAACTCTTTGGATTCTTTGTCGGGCAAGTGA
TGAAAAACTCTAAAGGCGCAAATCCAGCAAGGGTAAATGAGCTTTTAAAAGAAAAATTAGGGTAG

Upstream 100 bases:

>100_bases
TATTATATAAAAAATTTTTAAGAATTTATAAATATTTTATGCCTATTAGCCTATACTAGCATTTTATATTCCAATATTTT
CAACACAAAAGGCTATGAAA

Downstream 100 bases:

>100_bases
TATGAACAATGGAAGAGAATATTATAGCTTTTAGCGGGGGGGGGGGATAGAGAAAATTCTTATTGCCCTACATACCCGCA
GAATCTTAAATTCACCACAA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MSAYETIIGLEVHVQLNTKTKIFCFCATSFGDEPNKNVCPTCLGLPGALPVLNREAVKKAISFGTAINATINQNSVFARK
NYFYPDLPKAYQISQFEIPIVGRGSIEIECNNQTKTIGVTRAHLEEDAGKNIHENNYSKVDLNRACTPLLEIVSEPDMRS
SDEAIAYLKKLHSIVRFLGISDANMQEGSFRCDANVSIRPKGDCKLYTRVEIKNLNSFKFIQKAIEYEVERQIEAWEEGK
YQSEVVQETRLFDTAKGITRSMRGKEEAADYRYFPDPDLLPVFIDENLMREGVKIPEMPDEKRERYINTLGLKPYDAGVL
TSSLELALYFESMLEEGASAKGALTWLTTELLGRLKGENTLQTCGVDSKTLATLVKRIEEGKISGKSGKEILDVLMEKGG
DVDSLIDSMGLAQINDDGAIIAVIESVLSANADKVAEYKSGKDKLFGFFVGQVMKNSKGANPARVNELLKEKLG

Sequences:

>Translated_474_residues
MSAYETIIGLEVHVQLNTKTKIFCFCATSFGDEPNKNVCPTCLGLPGALPVLNREAVKKAISFGTAINATINQNSVFARK
NYFYPDLPKAYQISQFEIPIVGRGSIEIECNNQTKTIGVTRAHLEEDAGKNIHENNYSKVDLNRACTPLLEIVSEPDMRS
SDEAIAYLKKLHSIVRFLGISDANMQEGSFRCDANVSIRPKGDCKLYTRVEIKNLNSFKFIQKAIEYEVERQIEAWEEGK
YQSEVVQETRLFDTAKGITRSMRGKEEAADYRYFPDPDLLPVFIDENLMREGVKIPEMPDEKRERYINTLGLKPYDAGVL
TSSLELALYFESMLEEGASAKGALTWLTTELLGRLKGENTLQTCGVDSKTLATLVKRIEEGKISGKSGKEILDVLMEKGG
DVDSLIDSMGLAQINDDGAIIAVIESVLSANADKVAEYKSGKDKLFGFFVGQVMKNSKGANPARVNELLKEKLG
>Mature_473_residues
SAYETIIGLEVHVQLNTKTKIFCFCATSFGDEPNKNVCPTCLGLPGALPVLNREAVKKAISFGTAINATINQNSVFARKN
YFYPDLPKAYQISQFEIPIVGRGSIEIECNNQTKTIGVTRAHLEEDAGKNIHENNYSKVDLNRACTPLLEIVSEPDMRSS
DEAIAYLKKLHSIVRFLGISDANMQEGSFRCDANVSIRPKGDCKLYTRVEIKNLNSFKFIQKAIEYEVERQIEAWEEGKY
QSEVVQETRLFDTAKGITRSMRGKEEAADYRYFPDPDLLPVFIDENLMREGVKIPEMPDEKRERYINTLGLKPYDAGVLT
SSLELALYFESMLEEGASAKGALTWLTTELLGRLKGENTLQTCGVDSKTLATLVKRIEEGKISGKSGKEILDVLMEKGGD
VDSLIDSMGLAQINDDGAIIAVIESVLSANADKVAEYKSGKDKLFGFFVGQVMKNSKGANPARVNELLKEKLG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=497, Percent_Identity=34.6076458752515, Blast_Score=278, Evalue=9e-75,
Organism=Caenorhabditis elegans, GI115534094, Length=300, Percent_Identity=38, Blast_Score=182, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6319391, Length=507, Percent_Identity=32.1499013806706, Blast_Score=216, Evalue=9e-57,
Organism=Drosophila melanogaster, GI45550808, Length=494, Percent_Identity=31.9838056680162, Blast_Score=234, Evalue=9e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_HELHP (Q7VI13)

Other databases:

- EMBL:   AE017125
- RefSeq:   NP_860327.1
- ProteinModelPortal:   Q7VI13
- SMR:   Q7VI13
- GeneID:   1492485
- GenomeReviews:   AE017125_GR
- KEGG:   hhe:HH0796
- NMPDR:   fig|235279.1.peg.796
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- BioCyc:   HHEP235279:HH_0796-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 52622; Mature: 52490

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAYETIIGLEVHVQLNTKTKIFCFCATSFGDEPNKNVCPTCLGLPGALPVLNREAVKKA
CCCHHEEEEEEEEEEECCCEEEEEEEECCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHH
ISFGTAINATINQNSVFARKNYFYPDLPKAYQISQFEIPIVGRGSIEIECNNQTKTIGVT
HHHCCEEEEEECCCCEEEECCCCCCCCCCCEECCEEEEEEEECCEEEEEECCCEEEEEEE
RAHLEEDAGKNIHENNYSKVDLNRACTPLLEIVSEPDMRSSDEAIAYLKKLHSIVRFLGI
HHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCC
SDANMQEGSFRCDANVSIRPKGDCKLYTRVEIKNLNSFKFIQKAIEYEVERQIEAWEEGK
CCCCCCCCCEEECCCEEECCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCC
YQSEVVQETRLFDTAKGITRSMRGKEEAADYRYFPDPDLLPVFIDENLMREGVKIPEMPD
HHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCEEEEECCHHHHCCCCCCCCCC
EKRERYINTLGLKPYDAGVLTSSLELALYFESMLEEGASAKGALTWLTTELLGRLKGENT
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCC
LQTCGVDSKTLATLVKRIEEGKISGKSGKEILDVLMEKGGDVDSLIDSMGLAQINDDGAI
HHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHCCCEEECCCCHH
IAVIESVLSANADKVAEYKSGKDKLFGFFVGQVMKNSKGANPARVNELLKEKLG
HHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SAYETIIGLEVHVQLNTKTKIFCFCATSFGDEPNKNVCPTCLGLPGALPVLNREAVKKA
CCHHEEEEEEEEEEECCCEEEEEEEECCCCCCCCCCCCCHHHCCCCCCCCCCHHHHHHH
ISFGTAINATINQNSVFARKNYFYPDLPKAYQISQFEIPIVGRGSIEIECNNQTKTIGVT
HHHCCEEEEEECCCCEEEECCCCCCCCCCCEECCEEEEEEEECCEEEEEECCCEEEEEEE
RAHLEEDAGKNIHENNYSKVDLNRACTPLLEIVSEPDMRSSDEAIAYLKKLHSIVRFLGI
HHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCC
SDANMQEGSFRCDANVSIRPKGDCKLYTRVEIKNLNSFKFIQKAIEYEVERQIEAWEEGK
CCCCCCCCCEEECCCEEECCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCC
YQSEVVQETRLFDTAKGITRSMRGKEEAADYRYFPDPDLLPVFIDENLMREGVKIPEMPD
HHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCEEEEECCHHHHCCCCCCCCCC
EKRERYINTLGLKPYDAGVLTSSLELALYFESMLEEGASAKGALTWLTTELLGRLKGENT
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCC
LQTCGVDSKTLATLVKRIEEGKISGKSGKEILDVLMEKGGDVDSLIDSMGLAQINDDGAI
HHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHCCCEEECCCCHH
IAVIESVLSANADKVAEYKSGKDKLFGFFVGQVMKNSKGANPARVNELLKEKLG
HHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12810954