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The map label for this gene is aspA

Identifier: 32266221

GI number: 32266221

Start: 701495

End: 702934

Strand: Direct

Name: aspA

Synonym: HH0722

Alternate gene names: 32266221

Gene position: 701495-702934 (Clockwise)

Preceding gene: 32266220

Following gene: 32266222

Centisome position: 38.99

GC content: 38.12

Gene sequence:

>1440_bases
ATGGGCAAACGAGTAGAACACGATTTTATTGGCGAACTAGAGATTGCCGATGATATGTATTATGGTGTGCAAACCTTTAG
AGCATTACAAAATTTCAATATTACAGGCGATAAGCTAAGTAGCTATCCAAGCTTTATCAAGGCTTTTGCGCAAGTAAAAA
AAGCTGCTTGTTTGGCAAATTATGAACTTAAATTGCTTGATGAGCAAAAAAAAGATGCTATTTGCAAAGCTTGTGATAGG
CTTATTGCAGGCGAGCTAAGAGAGCAATTTGTAGTAGATATGCTTCAAGGTGGAGCTGGAACAAGCACAAATATGAACAC
AAATGAAGTTGTAGCAAATTTGGCATTAGAGATTATGGGACATAAAAAAGGTGAATATCAATACTGCCACCCAAATGACC
ACGTCAATCTCTCACAATCTACAAATGATTCATATCCCACTGCTATTCACGTAGCACTATATGACGAGCTTTCAGCTCTT
GCCAAAGATATGGAAGTGCTAAAAGAATCTTTTAATAAAAAATCTCAAGAATTTAAAGATGTGCTTAAAATGGGGCGAAC
TCAACTTCAAGATGCTGTGCCTATGACTTTAGGACAAGAGTTTCACACTTTTGCAGTGATGATGGGAGAAGATATTGCGC
GTGTGCTTGAAGCAAGAAACCTTGTAACTGAAATCAATATGGGTGGGACCGCAATTGGGACAGGCATAAACTCACACCCT
GATTATCCTAAAATTGTCCAAAAGAAACTCTGTGAGGTTACAGGACACCCTTTCAAAACTGCAGATGACCTCATTGAGGC
CACACAAGATACAGGTGCGTATGTGCAAGTAAGTGGTGTGCTTAAAAGAGTAGCTGTGAAACTCTCTAAAGTCTGTAATG
ACTTACGACTACTAAGCTCTGGACCAAGAGCGGGGCTTAATGAAATCAACCTCCCCAAAATGCAGCCCGGAAGCTCCATT
ATGCCCGGTAAAGTAAATCCTGTAATCCCTGAAGTTGTTAATCAAGTATGCTATGCAGTGATTGGTAATGATGTAACTGT
GAGCTTTGCAAGTGAGGGCGGACAATTGCAGCTTAATGTATTTGAGCCTGTGATTGCTTATGGATTATTTAACTCTATTT
CTATGATGAGAAATGCTATGCTTACACTTGCAAGCAAATGTATTGACGGAATCACTGCAAATGAAAAGATTTGTAGTGAT
TTTGTTTATAATAGCATTGGTATTGTTACAGCTCTTAATCCTTATATTGGCTATGAGAATTCTGCATCAATTGCTAAAGA
ATCTTTACAAACAGGCAAACCTGTTAAAGAAATTGCATTAGAAAGAAAGCTTTTAAGCGAAGAGCAACTCAATGAGATTT
TTCAACCAAAAAATATGCTCAATCCACATATGAGCGCAGAAGATAAGGCGAAATTCCAAAAAAATTCGCCTTTTGCATAA

Upstream 100 bases:

>100_bases
AACTATTCAAGTTTAGTTTAATCTTTGAGAATATATAATTCTTGCAAATTATCTAATGATTCGTGATAATTTACCTATAT
AATACTTACAGGAGTTTAAA

Downstream 100 bases:

>100_bases
TCTCTTTGTAAAGTGTTTTTAGAGGAGTAGCCAAAGGCACTTCTTTAAAACTTACAATTTCATTTTTTAAGGATTTTTTA
TGGAAATAATATTACTGGTT

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MGKRVEHDFIGELEIADDMYYGVQTFRALQNFNITGDKLSSYPSFIKAFAQVKKAACLANYELKLLDEQKKDAICKACDR
LIAGELREQFVVDMLQGGAGTSTNMNTNEVVANLALEIMGHKKGEYQYCHPNDHVNLSQSTNDSYPTAIHVALYDELSAL
AKDMEVLKESFNKKSQEFKDVLKMGRTQLQDAVPMTLGQEFHTFAVMMGEDIARVLEARNLVTEINMGGTAIGTGINSHP
DYPKIVQKKLCEVTGHPFKTADDLIEATQDTGAYVQVSGVLKRVAVKLSKVCNDLRLLSSGPRAGLNEINLPKMQPGSSI
MPGKVNPVIPEVVNQVCYAVIGNDVTVSFASEGGQLQLNVFEPVIAYGLFNSISMMRNAMLTLASKCIDGITANEKICSD
FVYNSIGIVTALNPYIGYENSASIAKESLQTGKPVKEIALERKLLSEEQLNEIFQPKNMLNPHMSAEDKAKFQKNSPFA

Sequences:

>Translated_479_residues
MGKRVEHDFIGELEIADDMYYGVQTFRALQNFNITGDKLSSYPSFIKAFAQVKKAACLANYELKLLDEQKKDAICKACDR
LIAGELREQFVVDMLQGGAGTSTNMNTNEVVANLALEIMGHKKGEYQYCHPNDHVNLSQSTNDSYPTAIHVALYDELSAL
AKDMEVLKESFNKKSQEFKDVLKMGRTQLQDAVPMTLGQEFHTFAVMMGEDIARVLEARNLVTEINMGGTAIGTGINSHP
DYPKIVQKKLCEVTGHPFKTADDLIEATQDTGAYVQVSGVLKRVAVKLSKVCNDLRLLSSGPRAGLNEINLPKMQPGSSI
MPGKVNPVIPEVVNQVCYAVIGNDVTVSFASEGGQLQLNVFEPVIAYGLFNSISMMRNAMLTLASKCIDGITANEKICSD
FVYNSIGIVTALNPYIGYENSASIAKESLQTGKPVKEIALERKLLSEEQLNEIFQPKNMLNPHMSAEDKAKFQKNSPFA
>Mature_478_residues
GKRVEHDFIGELEIADDMYYGVQTFRALQNFNITGDKLSSYPSFIKAFAQVKKAACLANYELKLLDEQKKDAICKACDRL
IAGELREQFVVDMLQGGAGTSTNMNTNEVVANLALEIMGHKKGEYQYCHPNDHVNLSQSTNDSYPTAIHVALYDELSALA
KDMEVLKESFNKKSQEFKDVLKMGRTQLQDAVPMTLGQEFHTFAVMMGEDIARVLEARNLVTEINMGGTAIGTGINSHPD
YPKIVQKKLCEVTGHPFKTADDLIEATQDTGAYVQVSGVLKRVAVKLSKVCNDLRLLSSGPRAGLNEINLPKMQPGSSIM
PGKVNPVIPEVVNQVCYAVIGNDVTVSFASEGGQLQLNVFEPVIAYGLFNSISMMRNAMLTLASKCIDGITANEKICSDF
VYNSIGIVTALNPYIGYENSASIAKESLQTGKPVKEIALERKLLSEEQLNEIFQPKNMLNPHMSAEDKAKFQKNSPFA

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=460, Percent_Identity=41.5217391304348, Blast_Score=365, Evalue=1e-101,
Organism=Escherichia coli, GI87082375, Length=465, Percent_Identity=56.5591397849462, Blast_Score=536, Evalue=1e-153,
Organism=Escherichia coli, GI1787896, Length=458, Percent_Identity=40.3930131004367, Blast_Score=361, Evalue=1e-101,
Organism=Escherichia coli, GI1790398, Length=315, Percent_Identity=22.5396825396825, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17553882, Length=460, Percent_Identity=43.4782608695652, Blast_Score=395, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI32565146, Length=327, Percent_Identity=42.2018348623853, Blast_Score=280, Evalue=9e-76,
Organism=Saccharomyces cerevisiae, GI6324993, Length=460, Percent_Identity=41.0869565217391, Blast_Score=369, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24640177, Length=461, Percent_Identity=42.5162689804772, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24640179, Length=461, Percent_Identity=42.5162689804772, Blast_Score=374, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24662684, Length=465, Percent_Identity=42.1505376344086, Blast_Score=368, Evalue=1e-102,
Organism=Drosophila melanogaster, GI78710009, Length=463, Percent_Identity=42.7645788336933, Blast_Score=368, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24583245, Length=462, Percent_Identity=38.0952380952381, Blast_Score=328, Evalue=5e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 52702; Mature: 52571

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKRVEHDFIGELEIADDMYYGVQTFRALQNFNITGDKLSSYPSFIKAFAQVKKAACLAN
CCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
YELKLLDEQKKDAICKACDRLIAGELREQFVVDMLQGGAGTSTNMNTNEVVANLALEIMG
CHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHC
HKKGEYQYCHPNDHVNLSQSTNDSYPTAIHVALYDELSALAKDMEVLKESFNKKSQEFKD
CCCCCCCEECCCCCEECCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
VLKMGRTQLQDAVPMTLGQEFHTFAVMMGEDIARVLEARNLVTEINMGGTAIGTGINSHP
HHHHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCC
DYPKIVQKKLCEVTGHPFKTADDLIEATQDTGAYVQVSGVLKRVAVKLSKVCNDLRLLSS
CCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCC
GPRAGLNEINLPKMQPGSSIMPGKVNPVIPEVVNQVCYAVIGNDVTVSFASEGGQLQLNV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEH
FEPVIAYGLFNSISMMRNAMLTLASKCIDGITANEKICSDFVYNSIGIVTALNPYIGYEN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
SASIAKESLQTGKPVKEIALERKLLSEEQLNEIFQPKNMLNPHMSAEDKAKFQKNSPFA
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHCCCCCCC
>Mature Secondary Structure 
GKRVEHDFIGELEIADDMYYGVQTFRALQNFNITGDKLSSYPSFIKAFAQVKKAACLAN
CCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
YELKLLDEQKKDAICKACDRLIAGELREQFVVDMLQGGAGTSTNMNTNEVVANLALEIMG
CHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHC
HKKGEYQYCHPNDHVNLSQSTNDSYPTAIHVALYDELSALAKDMEVLKESFNKKSQEFKD
CCCCCCCEECCCCCEECCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
VLKMGRTQLQDAVPMTLGQEFHTFAVMMGEDIARVLEARNLVTEINMGGTAIGTGINSHP
HHHHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCC
DYPKIVQKKLCEVTGHPFKTADDLIEATQDTGAYVQVSGVLKRVAVKLSKVCNDLRLLSS
CCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCC
GPRAGLNEINLPKMQPGSSIMPGKVNPVIPEVVNQVCYAVIGNDVTVSFASEGGQLQLNV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCEEEEEH
FEPVIAYGLFNSISMMRNAMLTLASKCIDGITANEKICSDFVYNSIGIVTALNPYIGYEN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCC
SASIAKESLQTGKPVKEIALERKLLSEEQLNEIFQPKNMLNPHMSAEDKAKFQKNSPFA
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]