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The map label for this gene is waaA

Identifier: 32266139

GI number: 32266139

Start: 618784

End: 620040

Strand: Direct

Name: waaA

Synonym: HH0640

Alternate gene names: 32266139

Gene position: 618784-620040 (Clockwise)

Preceding gene: 32266138

Following gene: 32266140

Centisome position: 34.39

GC content: 35.88

Gene sequence:

>1257_bases
ATGGATAGAGCAGATTCTACAAAAGATTTAGATAAAAATAAAACTCCTCGTTTTCCTTTTACATATTATTGTCTTTGTAT
AATACTCTATCTTATAGCACTACCTATTTTAATTGCAAATATATTTCGTGCAAAACATCGTGAATCTATTCCAGCACGTT
TTTTTTACTCATCGCTTGATTGTGAGCCACAATATTGGTTTCACGCGTGTTCTTTTGGCGAAATAAAATCTCTTGAACCC
CTTATCAACGCATCAAAAACTATGCCTTGCACCATTCTTATTACAACTATTACACATACAGGTTTTAAAGAAGCAAAACG
CCTATATCAAACGCATTCTGCAGATTCACAAGATACAGCCCATATTGTCGTGCGTTATTTACCTTTTGAAATATTTTTGC
CCTTATGGAGTAAATCTTGTCGCCAACTTAAAACTCTCGTGGTTACAGAAGCTGAAATATGGCAAATGCTTTTTTATCTT
GCGAAGTCTCATAGAGCACGCACTCTACTGATTAATGCCCGCATTTCTAATCGCTCACACAAAAATTATCAACGTTTTGC
GTGGTTTTATCAAGGCATTTTTAATCTTATTGATGAAGTGCTTGCTCAAAGTCAAATTGATAAGGAGCGTTTAGAGAATT
TAGGTGCACATAATGTTGAAGTTTTTGGTAATCTTAAAACACTCAATACTCCCTCACTTAGCGCACATTATACCAAACCT
TCCTCTGCTGTATTTATTGGCGCAAGCACACATAGAGGCGAAGAAAAGCTTATCCTTGAAGCCTTTAAAGCTCTTAAAAA
TGCTCAAAAATCATCTGATAACTCTGCACTTTTACTCATTGCACCGCGCCACCCAGAACGTTTTAAGGAAGTGTATGAAT
TAAGCCTACGCACTTTTACAAACACTACACTTTTCTCACAAACACATTTGAATGCTCATAATGCAGATGTAATTATTATT
GATACACTTGGAGAACTTAATAATCTCTATGCAATAAGTGATGTTGTCATACTTGGTGGTGGTTTTGCCAAAATAGGAGG
GCATAATCCGCTTGAGCCCGCTTATTTTCATACAAAGCTTATAAGTGGAGAACATATTTTTAATCAATACGCACTTTTTG
AGGAAATAGAACATTATGTGCTGATACCTCCCCAAAGCTTAAAAGATACGCTTCTTAGATGGGAATCTCTACCCAAGAGC
GCCATTAAAACAAATTCCCAAGACAAACTCACTACCCTTATACAAAAGATATACTGA

Upstream 100 bases:

>100_bases
TTCAAAAAGCACTCAAACACCAACAAATACAGCTGAAGAAAAACCTCAAAGCAAGAAAAAAACTAAAGCATAGAATCTAA
GAGCTTTTTGAGGAATTGTT

Downstream 100 bases:

>100_bases
TGTATAAAGATAAGGCTTACAAAGTCCTCGCCCGCACACATAATATTTCTCATAATCAAGCTAAATCTCTTATTGATAAA
GGACTTGTCTTCGCTGCAGG

Product: 3-deoxy-D-manno-octulosonic-acid transferase

Products: NA

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MDRADSTKDLDKNKTPRFPFTYYCLCIILYLIALPILIANIFRAKHRESIPARFFYSSLDCEPQYWFHACSFGEIKSLEP
LINASKTMPCTILITTITHTGFKEAKRLYQTHSADSQDTAHIVVRYLPFEIFLPLWSKSCRQLKTLVVTEAEIWQMLFYL
AKSHRARTLLINARISNRSHKNYQRFAWFYQGIFNLIDEVLAQSQIDKERLENLGAHNVEVFGNLKTLNTPSLSAHYTKP
SSAVFIGASTHRGEEKLILEAFKALKNAQKSSDNSALLLIAPRHPERFKEVYELSLRTFTNTTLFSQTHLNAHNADVIII
DTLGELNNLYAISDVVILGGGFAKIGGHNPLEPAYFHTKLISGEHIFNQYALFEEIEHYVLIPPQSLKDTLLRWESLPKS
AIKTNSQDKLTTLIQKIY

Sequences:

>Translated_418_residues
MDRADSTKDLDKNKTPRFPFTYYCLCIILYLIALPILIANIFRAKHRESIPARFFYSSLDCEPQYWFHACSFGEIKSLEP
LINASKTMPCTILITTITHTGFKEAKRLYQTHSADSQDTAHIVVRYLPFEIFLPLWSKSCRQLKTLVVTEAEIWQMLFYL
AKSHRARTLLINARISNRSHKNYQRFAWFYQGIFNLIDEVLAQSQIDKERLENLGAHNVEVFGNLKTLNTPSLSAHYTKP
SSAVFIGASTHRGEEKLILEAFKALKNAQKSSDNSALLLIAPRHPERFKEVYELSLRTFTNTTLFSQTHLNAHNADVIII
DTLGELNNLYAISDVVILGGGFAKIGGHNPLEPAYFHTKLISGEHIFNQYALFEEIEHYVLIPPQSLKDTLLRWESLPKS
AIKTNSQDKLTTLIQKIY
>Mature_418_residues
MDRADSTKDLDKNKTPRFPFTYYCLCIILYLIALPILIANIFRAKHRESIPARFFYSSLDCEPQYWFHACSFGEIKSLEP
LINASKTMPCTILITTITHTGFKEAKRLYQTHSADSQDTAHIVVRYLPFEIFLPLWSKSCRQLKTLVVTEAEIWQMLFYL
AKSHRARTLLINARISNRSHKNYQRFAWFYQGIFNLIDEVLAQSQIDKERLENLGAHNVEVFGNLKTLNTPSLSAHYTKP
SSAVFIGASTHRGEEKLILEAFKALKNAQKSSDNSALLLIAPRHPERFKEVYELSLRTFTNTTLFSQTHLNAHNADVIII
DTLGELNNLYAISDVVILGGGFAKIGGHNPLEPAYFHTKLISGEHIFNQYALFEEIEHYVLIPPQSLKDTLLRWESLPKS
AIKTNSQDKLTTLIQKIY

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers a single molecule of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=362, Percent_Identity=30.3867403314917, Blast_Score=138, Evalue=9e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.-

Molecular weight: Translated: 47887; Mature: 47887

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRADSTKDLDKNKTPRFPFTYYCLCIILYLIALPILIANIFRAKHRESIPARFFYSSLD
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCC
CEPQYWFHACSFGEIKSLEPLINASKTMPCTILITTITHTGFKEAKRLYQTHSADSQDTA
CCCCHHEEECCCCCCCCCCHHHCCCCCCCEEEEEEEHHCCCHHHHHHHHHHCCCCCCCHH
HIVVRYLPFEIFLPLWSKSCRQLKTLVVTEAEIWQMLFYLAKSHRARTLLINARISNRSH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCC
KNYQRFAWFYQGIFNLIDEVLAQSQIDKERLENLGAHNVEVFGNLKTLNTPSLSAHYTKP
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEECEEECCCCCCCEEECCC
SSAVFIGASTHRGEEKLILEAFKALKNAQKSSDNSALLLIAPRHPERFKEVYELSLRTFT
CCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHC
NTTLFSQTHLNAHNADVIIIDTLGELNNLYAISDVVILGGGFAKIGGHNPLEPAYFHTKL
CCEEEEECCCCCCCCCEEEEECCCCCCCCEEEEHEEEECCCCCCCCCCCCCCCHHHHHHH
ISGEHIFNQYALFEEIEHYVLIPPQSLKDTLLRWESLPKSAIKTNSQDKLTTLIQKIY
CCCHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHC
>Mature Secondary Structure
MDRADSTKDLDKNKTPRFPFTYYCLCIILYLIALPILIANIFRAKHRESIPARFFYSSLD
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCC
CEPQYWFHACSFGEIKSLEPLINASKTMPCTILITTITHTGFKEAKRLYQTHSADSQDTA
CCCCHHEEECCCCCCCCCCHHHCCCCCCCEEEEEEEHHCCCHHHHHHHHHHCCCCCCCHH
HIVVRYLPFEIFLPLWSKSCRQLKTLVVTEAEIWQMLFYLAKSHRARTLLINARISNRSH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCC
KNYQRFAWFYQGIFNLIDEVLAQSQIDKERLENLGAHNVEVFGNLKTLNTPSLSAHYTKP
CHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEECEEECCCCCCCEEECCC
SSAVFIGASTHRGEEKLILEAFKALKNAQKSSDNSALLLIAPRHPERFKEVYELSLRTFT
CCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHC
NTTLFSQTHLNAHNADVIIIDTLGELNNLYAISDVVILGGGFAKIGGHNPLEPAYFHTKL
CCEEEEECCCCCCCCCEEEEECCCCCCCCEEEEHEEEECCCCCCCCCCCCCCCHHHHHHH
ISGEHIFNQYALFEEIEHYVLIPPQSLKDTLLRWESLPKSAIKTNSQDKLTTLIQKIY
CCCHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHCCHHHHCCCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952982; 7542800 [H]