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The map label for this gene is metY

Identifier: 32266135

GI number: 32266135

Start: 614897

End: 616189

Strand: Direct

Name: metY

Synonym: HH0636

Alternate gene names: 32266135

Gene position: 614897-616189 (Clockwise)

Preceding gene: 32266133

Following gene: 32266136

Centisome position: 34.18

GC content: 36.97

Gene sequence:

>1293_bases
ATGGCAAATCTCTCTCACCACAATCTTTCACAAGATACGCTTGCACTTCACGCAGGTTATACTTATGACACACAGCGCAC
AATCAGCGTGCCAATTTATCAAAATACTGCTTATAGTTTTGAGAGCTTAGAACAAGCTGCTGCACGATTCGGACTTCAAG
AGATTGGTAATATTTATACACGCTTGAGCAATCCTACCACCGATGTTCTAGGTGCGCGCTTAGCTGCAGTTGAGGGAGGA
ATATTTGGTGTGCCTACTGCTAGTGGGAGTGCTGCAATTTTTTATGCGATTGTTAATTGTGCGCAAAGTGGCGATAATAT
CGTATTTTCCAATAAAATCTATGGTGGCACACAAACACTATTCGTGCATACTTTAAAACGATTTGGCATACAAACAAAAG
TTTTTGATATTGACAATATTGAATCTTCTTTGGAATCTGTCATAGATGATAAAACAAAAGCGATTTTTTTTGAAAGTATT
TCAAACCCACAAATATCTATTGCCGATACGGAGAAAATTACCGCTATTGCTAAAAAACATAAAATTATTAGCATTTGTGA
TAATACCATTCCCACCGCATTTTTACATAAACCTTTTGATTATGGCGTGGATATTAGCATATATAGTCTAAGTAAGTATA
TTAATGGGCAAGGAAGTGCGCTTGGTGGAGCAATTATTGAGCGATATGGATTAAATGAATTAATTAAGGATAATCCGCGC
TATGAGGTATTTAACACACCTGATGCAAGCTATCACGGATTAATATATGCAAATCTTCCTTTGCCTTGCTTTAGTATCCG
GCTTATTACAGAATGGCTTAGAAACATTGGCGCTACTCTCTCTCCACAAGCATCGTGGATTATTCTACAAGGCTTAGAAA
CTTTAGAGTTGCGTATTAAAAAACATAGTCAAAATGCCCTTAAAGTCGCGGAATTTTTACAATCACACCCTAAGGTGCAA
AGTGTGGCTTATGCTGGATTAACAAGTAACCCTTACAATAGACTACTTCAAAAATATTACAAAGATTCCCAAGCAAGTGG
GCTTATCAGTTTTGAGGCGCAAAGTTTTGAAGAGGCACAAAAAATTTGTAATGCTACAGAAATTTTTGCAGTCGTAGTTA
ATATTGGGGATTCAAAATCACTTATCATTCACCCCGCTTCCACAACACATTCGCAACTTAATGCAGATGAACTCTCAAGC
GCAGGTATCACACCTTGCACTATACGCCTTAGCATTGGTTTAGAATCTGCAGATGATTTAATACAAGATTTAAAAAAAGC
TTTAGAAAAATAA

Upstream 100 bases:

>100_bases
TTTATCCTTTTTAGCATATTTATATATATTACATATTGTAATCTTATTTTTTTGTGATATTATTAAATTCAAAAAATAAC
AAAATACAACGAGGTATAAT

Downstream 100 bases:

>100_bases
GGAAAACTATGCCACTCATTATCCCAGAAGATATTCCCGCTTATAAATTCCTCAATCAAAATGCTTTTGTTATGGGTTCT
CAAAGGGCAAAACATCAAGA

Product: O-acetylhomoserine (thiol)-lyase MetY

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MANLSHHNLSQDTLALHAGYTYDTQRTISVPIYQNTAYSFESLEQAAARFGLQEIGNIYTRLSNPTTDVLGARLAAVEGG
IFGVPTASGSAAIFYAIVNCAQSGDNIVFSNKIYGGTQTLFVHTLKRFGIQTKVFDIDNIESSLESVIDDKTKAIFFESI
SNPQISIADTEKITAIAKKHKIISICDNTIPTAFLHKPFDYGVDISIYSLSKYINGQGSALGGAIIERYGLNELIKDNPR
YEVFNTPDASYHGLIYANLPLPCFSIRLITEWLRNIGATLSPQASWIILQGLETLELRIKKHSQNALKVAEFLQSHPKVQ
SVAYAGLTSNPYNRLLQKYYKDSQASGLISFEAQSFEEAQKICNATEIFAVVVNIGDSKSLIIHPASTTHSQLNADELSS
AGITPCTIRLSIGLESADDLIQDLKKALEK

Sequences:

>Translated_430_residues
MANLSHHNLSQDTLALHAGYTYDTQRTISVPIYQNTAYSFESLEQAAARFGLQEIGNIYTRLSNPTTDVLGARLAAVEGG
IFGVPTASGSAAIFYAIVNCAQSGDNIVFSNKIYGGTQTLFVHTLKRFGIQTKVFDIDNIESSLESVIDDKTKAIFFESI
SNPQISIADTEKITAIAKKHKIISICDNTIPTAFLHKPFDYGVDISIYSLSKYINGQGSALGGAIIERYGLNELIKDNPR
YEVFNTPDASYHGLIYANLPLPCFSIRLITEWLRNIGATLSPQASWIILQGLETLELRIKKHSQNALKVAEFLQSHPKVQ
SVAYAGLTSNPYNRLLQKYYKDSQASGLISFEAQSFEEAQKICNATEIFAVVVNIGDSKSLIIHPASTTHSQLNADELSS
AGITPCTIRLSIGLESADDLIQDLKKALEK
>Mature_429_residues
ANLSHHNLSQDTLALHAGYTYDTQRTISVPIYQNTAYSFESLEQAAARFGLQEIGNIYTRLSNPTTDVLGARLAAVEGGI
FGVPTASGSAAIFYAIVNCAQSGDNIVFSNKIYGGTQTLFVHTLKRFGIQTKVFDIDNIESSLESVIDDKTKAIFFESIS
NPQISIADTEKITAIAKKHKIISICDNTIPTAFLHKPFDYGVDISIYSLSKYINGQGSALGGAIIERYGLNELIKDNPRY
EVFNTPDASYHGLIYANLPLPCFSIRLITEWLRNIGATLSPQASWIILQGLETLELRIKKHSQNALKVAEFLQSHPKVQS
VAYAGLTSNPYNRLLQKYYKDSQASGLISFEAQSFEEAQKICNATEIFAVVVNIGDSKSLIIHPASTTHSQLNADELSSA
GITPCTIRLSIGLESADDLIQDLKKALEK

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=406, Percent_Identity=29.5566502463054, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI299473759, Length=406, Percent_Identity=26.8472906403941, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI25453487, Length=405, Percent_Identity=26.4197530864197, Blast_Score=125, Evalue=8e-29,
Organism=Escherichia coli, GI1790375, Length=421, Percent_Identity=28.0285035629454, Blast_Score=171, Evalue=8e-44,
Organism=Escherichia coli, GI1789383, Length=429, Percent_Identity=24.7086247086247, Blast_Score=112, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71980708, Length=409, Percent_Identity=32.2738386308068, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17538055, Length=367, Percent_Identity=31.0626702997275, Blast_Score=174, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI17560056, Length=375, Percent_Identity=32, Blast_Score=168, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6323333, Length=438, Percent_Identity=38.3561643835616, Blast_Score=267, Evalue=2e-72,
Organism=Saccharomyces cerevisiae, GI6319307, Length=425, Percent_Identity=29.1764705882353, Blast_Score=180, Evalue=5e-46,
Organism=Saccharomyces cerevisiae, GI6321254, Length=447, Percent_Identity=24.6085011185682, Blast_Score=119, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6321170, Length=326, Percent_Identity=26.9938650306748, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321904, Length=384, Percent_Identity=23.9583333333333, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI19922554, Length=424, Percent_Identity=30.188679245283, Blast_Score=179, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 47127; Mature: 46996

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANLSHHNLSQDTLALHAGYTYDTQRTISVPIYQNTAYSFESLEQAAARFGLQEIGNIYT
CCCCCCCCCCCCCEEEECCCEECCCEEEEEEEECCCCCCHHHHHHHHHHHCHHHHHHHHH
RLSNPTTDVLGARLAAVEGGIFGVPTASGSAAIFYAIVNCAQSGDNIVFSNKIYGGTQTL
HCCCCCHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHCCCCCEEEECEECCCHHHH
FVHTLKRFGIQTKVFDIDNIESSLESVIDDKTKAIFFESISNPQISIADTEKITAIAKKH
HHHHHHHHCCEEEEEECHHHHHHHHHHHCCHHHEEEEECCCCCEEEEECCHHHHHHHHHC
KIISICDNTIPTAFLHKPFDYGVDISIYSLSKYINGQGSALGGAIIERYGLNELIKDNPR
CEEEECCCCCCHHHHCCCCCCCCCEEEEEHHHHHCCCCCHHHHHHHHHHCCHHHHCCCCC
YEVFNTPDASYHGLIYANLPLPCFSIRLITEWLRNIGATLSPQASWIILQGLETLELRIK
EEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHH
KHSQNALKVAEFLQSHPKVQSVAYAGLTSNPYNRLLQKYYKDSQASGLISFEAQSFEEAQ
HCCCHHHHHHHHHHHCCCHHHEEECCCCCCHHHHHHHHHHCCCCCCCEEEECHHHHHHHH
KICNATEIFAVVVNIGDSKSLIIHPASTTHSQLNADELSSAGITPCTIRLSIGLESADDL
HHHCHHEEEEEEEECCCCCEEEEECCCCCCCCCCHHHHHCCCCCEEEEEEEECCCCHHHH
IQDLKKALEK
HHHHHHHHCC
>Mature Secondary Structure 
ANLSHHNLSQDTLALHAGYTYDTQRTISVPIYQNTAYSFESLEQAAARFGLQEIGNIYT
CCCCCCCCCCCCEEEECCCEECCCEEEEEEEECCCCCCHHHHHHHHHHHCHHHHHHHHH
RLSNPTTDVLGARLAAVEGGIFGVPTASGSAAIFYAIVNCAQSGDNIVFSNKIYGGTQTL
HCCCCCHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHCCCCCEEEECEECCCHHHH
FVHTLKRFGIQTKVFDIDNIESSLESVIDDKTKAIFFESISNPQISIADTEKITAIAKKH
HHHHHHHHCCEEEEEECHHHHHHHHHHHCCHHHEEEEECCCCCEEEEECCHHHHHHHHHC
KIISICDNTIPTAFLHKPFDYGVDISIYSLSKYINGQGSALGGAIIERYGLNELIKDNPR
CEEEECCCCCCHHHHCCCCCCCCCEEEEEHHHHHCCCCCHHHHHHHHHHCCHHHHCCCCC
YEVFNTPDASYHGLIYANLPLPCFSIRLITEWLRNIGATLSPQASWIILQGLETLELRIK
EEEECCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEECCHHHHHHHH
KHSQNALKVAEFLQSHPKVQSVAYAGLTSNPYNRLLQKYYKDSQASGLISFEAQSFEEAQ
HCCCHHHHHHHHHHHCCCHHHEEECCCCCCHHHHHHHHHHCCCCCCCEEEECHHHHHHHH
KICNATEIFAVVVNIGDSKSLIIHPASTTHSQLNADELSSAGITPCTIRLSIGLESADDL
HHHCHHEEEEEEEECCCCCEEEEECCCCCCCCCCHHHHHCCCCCEEEEEEEECCCCHHHH
IQDLKKALEK
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]