Click here to switch to the map view.

The map label for this gene is purN

Identifier: 32265969

GI number: 32265969

Start: 454156

End: 454731

Strand: Direct

Name: purN

Synonym: HH0470

Alternate gene names: 32265969

Gene position: 454156-454731 (Clockwise)

Preceding gene: 32265968

Following gene: 32265970

Centisome position: 25.24

GC content: 35.24

Gene sequence:

>576_bases
ATGCAAAACCTTATAGAATCTTTGCATAATAAAATATTTTATGATAATAATAACAATCCTGTGCATCTTGAAATCACTCT
TACTCTTTGTAATAATCCCAAAGCACAAGGTATTACACGCTCTGCTGCTCTTGCTATCCCTTGCACAATTATTAATCACA
AAGATTTCTCCTCGCGTATTGATTTTGATAATGCTATGATAGAAATACTCCGAGTGCATTCCATAGATATTGTGCTCTTA
GCAGGTTTTATGCGCATTCTTACATCTTCTTTTACACAAACCTTTCAAACAATCAATATCCACCCCTCTTTTCTGCCTGA
ACACAAAGGAGCTTATGCCATTAGAGAGAGTTTTAACTCTGCTCAATCCTATGGTGGAGTAAGTGTCCATTGGGTAAATG
AAGAACTTGATGGTGGAGAAATTATTTTGCAAGAAAAATTGCAAAAGATTCCAAATGAAAACCTTGAAGAATTTGAATCT
AAAATCCACGCCCTTGAATATTCTCTCTATCCACGCGCTATTTTGCTCGCACTTGGGTTAAAAGACTTAAACAAAAGTGT
AGAGGAATCAAAATGA

Upstream 100 bases:

>100_bases
TCTTAAAAAAGACTACCCACAATTTTTTATCAAAACATAGGTTTGCAGAGTGATGAGAAACATCAAATGTGCCATTTTAT
TTAGCGGAAACGGAAGCAAT

Downstream 100 bases:

>100_bases
GTTATCCCATTGAAACACTCAGTGCTTTTAGCATTTTTGTTCTCCTTATTCTATTTGCTTCGCCTATTAGTAGCCTTACG
CGTATTCCTATTGTAGTAGT

Product: GAR transformylase PurN

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MQNLIESLHNKIFYDNNNNPVHLEITLTLCNNPKAQGITRSAALAIPCTIINHKDFSSRIDFDNAMIEILRVHSIDIVLL
AGFMRILTSSFTQTFQTINIHPSFLPEHKGAYAIRESFNSAQSYGGVSVHWVNEELDGGEIILQEKLQKIPNENLEEFES
KIHALEYSLYPRAILLALGLKDLNKSVEESK

Sequences:

>Translated_191_residues
MQNLIESLHNKIFYDNNNNPVHLEITLTLCNNPKAQGITRSAALAIPCTIINHKDFSSRIDFDNAMIEILRVHSIDIVLL
AGFMRILTSSFTQTFQTINIHPSFLPEHKGAYAIRESFNSAQSYGGVSVHWVNEELDGGEIILQEKLQKIPNENLEEFES
KIHALEYSLYPRAILLALGLKDLNKSVEESK
>Mature_191_residues
MQNLIESLHNKIFYDNNNNPVHLEITLTLCNNPKAQGITRSAALAIPCTIINHKDFSSRIDFDNAMIEILRVHSIDIVLL
AGFMRILTSSFTQTFQTINIHPSFLPEHKGAYAIRESFNSAQSYGGVSVHWVNEELDGGEIILQEKLQKIPNENLEEFES
KIHALEYSLYPRAILLALGLKDLNKSVEESK

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=155, Percent_Identity=35.4838709677419, Blast_Score=114, Evalue=4e-26,
Organism=Homo sapiens, GI209869995, Length=155, Percent_Identity=35.4838709677419, Blast_Score=114, Evalue=4e-26,
Organism=Homo sapiens, GI209869993, Length=155, Percent_Identity=35.4838709677419, Blast_Score=114, Evalue=4e-26,
Organism=Escherichia coli, GI1788846, Length=159, Percent_Identity=30.8176100628931, Blast_Score=98, Evalue=3e-22,
Organism=Escherichia coli, GI1787483, Length=138, Percent_Identity=29.7101449275362, Blast_Score=68, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17567511, Length=187, Percent_Identity=32.0855614973262, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6320616, Length=166, Percent_Identity=29.5180722891566, Blast_Score=68, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24582400, Length=158, Percent_Identity=31.6455696202532, Blast_Score=117, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 21606; Mature: 21606

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNLIESLHNKIFYDNNNNPVHLEITLTLCNNPKAQGITRSAALAIPCTIINHKDFSSRI
CHHHHHHHHHCEEECCCCCEEEEEEEEEEECCCCCCCCCCCHHEEEEEEEECCCCCCCCC
DFDNAMIEILRVHSIDIVLLAGFMRILTSSFTQTFQTINIHPSFLPEHKGAYAIRESFNS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHH
AQSYGGVSVHWVNEELDGGEIILQEKLQKIPNENLEEFESKIHALEYSLYPRAILLALGL
HHHCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KDLNKSVEESK
HHHHHHHHCCC
>Mature Secondary Structure
MQNLIESLHNKIFYDNNNNPVHLEITLTLCNNPKAQGITRSAALAIPCTIINHKDFSSRI
CHHHHHHHHHCEEECCCCCEEEEEEEEEEECCCCCCCCCCCHHEEEEEEEECCCCCCCCC
DFDNAMIEILRVHSIDIVLLAGFMRILTSSFTQTFQTINIHPSFLPEHKGAYAIRESFNS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHH
AQSYGGVSVHWVNEELDGGEIILQEKLQKIPNENLEEFESKIHALEYSLYPRAILLALGL
HHHCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KDLNKSVEESK
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]