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The map label for this gene is proB

Identifier: 32265918

GI number: 32265918

Start: 404743

End: 405537

Strand: Direct

Name: proB

Synonym: HH0419

Alternate gene names: 32265918

Gene position: 404743-405537 (Clockwise)

Preceding gene: 32265917

Following gene: 32265919

Centisome position: 22.5

GC content: 37.36

Gene sequence:

>795_bases
ATGCAAAAGCCACGAATAGTTCTTAAAATTGGCTCTTCAAACCTTTGTAATGGCAAAATCATTGACAAAACCCAAATTAA
AGCATTAGCACAAATTATCAGTGAGCTCAAGATGCGTTTTGATGTGATTCTTGTCAGTTCCGGAGCAGTAGCAAGCGGTC
ATACTACGCTGCACATAGATAGAAATACTTTGCAAAACAAACAAGCCCTAGCAAGTATCGGTCAGCCTTTGCTAATGGAA
TCTTATCGTGAGGCATTAAAAGATTATGCAATTCCTACTGCACAATTGCTCCTTGTGTGGCGTGATTTCGATTCTCGCAA
AAATACAACTTTTGCAAAGGATACGATTGATACACTTCTAGCACACAATGCACTCCCTATTATTAATGAAAATGATACTA
TTGCCACAGATGAAATGGTTTTTGGCGACAATGATAGGCTTGGTGCTTATGTAACATATTATTTTGGTGCAAAACTCCTT
ATTATTTTAAGCGATATTGATGGCTATTTTGACAAAAATCCTCATCAATATGATGATGCACAAATCCTCCCTATAGTGCA
TTCTATTCCTTCTCAAGCATTAGAACAAACTCATAGCCCACACGGAGATTTTGCCACAGGAGGGATTGTTACCAAACTTA
TAGCAGCAGATTTTTTACTTCAACGCAAATGTATGATGTTTTTAAGCCACGGACGCAAACTTGATGTATTAAGAGATTTT
CTTCTACATAATAAACAGAGTTCGGGCACACTTTTTTGCCCTGCAGATTCTCAAGGTATAAAGACGCTTATATGA

Upstream 100 bases:

>100_bases
AGCAAATTATTCCTTGCAGTTGCGATATAAATTATAATTTCAAAGAAGTATTTTAGCGACCTAAAGATATAATCTCTCTT
TAATTTGAAGGAGAGATTAT

Downstream 100 bases:

>100_bases
AACTGATTCTCGCTGGAACACCTGCATTTGCATCGGCAATATTTACACCCATAATAAAAAGCAAAATATTTGATATTCTT
GCTCTTATCTGTCAGCCTGA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MQKPRIVLKIGSSNLCNGKIIDKTQIKALAQIISELKMRFDVILVSSGAVASGHTTLHIDRNTLQNKQALASIGQPLLME
SYREALKDYAIPTAQLLLVWRDFDSRKNTTFAKDTIDTLLAHNALPIINENDTIATDEMVFGDNDRLGAYVTYYFGAKLL
IILSDIDGYFDKNPHQYDDAQILPIVHSIPSQALEQTHSPHGDFATGGIVTKLIAADFLLQRKCMMFLSHGRKLDVLRDF
LLHNKQSSGTLFCPADSQGIKTLI

Sequences:

>Translated_264_residues
MQKPRIVLKIGSSNLCNGKIIDKTQIKALAQIISELKMRFDVILVSSGAVASGHTTLHIDRNTLQNKQALASIGQPLLME
SYREALKDYAIPTAQLLLVWRDFDSRKNTTFAKDTIDTLLAHNALPIINENDTIATDEMVFGDNDRLGAYVTYYFGAKLL
IILSDIDGYFDKNPHQYDDAQILPIVHSIPSQALEQTHSPHGDFATGGIVTKLIAADFLLQRKCMMFLSHGRKLDVLRDF
LLHNKQSSGTLFCPADSQGIKTLI
>Mature_264_residues
MQKPRIVLKIGSSNLCNGKIIDKTQIKALAQIISELKMRFDVILVSSGAVASGHTTLHIDRNTLQNKQALASIGQPLLME
SYREALKDYAIPTAQLLLVWRDFDSRKNTTFAKDTIDTLLAHNALPIINENDTIATDEMVFGDNDRLGAYVTYYFGAKLL
IILSDIDGYFDKNPHQYDDAQILPIVHSIPSQALEQTHSPHGDFATGGIVTKLIAADFLLQRKCMMFLSHGRKLDVLRDF
LLHNKQSSGTLFCPADSQGIKTLI

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate 5-kinase family

Homologues:

Organism=Homo sapiens, GI21361368, Length=263, Percent_Identity=28.1368821292776, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI62912457, Length=261, Percent_Identity=28.3524904214559, Blast_Score=83, Evalue=2e-16,
Organism=Escherichia coli, GI1786437, Length=251, Percent_Identity=31.8725099601594, Blast_Score=119, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17569969, Length=272, Percent_Identity=30.5147058823529, Blast_Score=107, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17569967, Length=272, Percent_Identity=30.5147058823529, Blast_Score=107, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6321822, Length=221, Percent_Identity=37.10407239819, Blast_Score=119, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6320506, Length=221, Percent_Identity=34.841628959276, Blast_Score=109, Evalue=4e-25,
Organism=Drosophila melanogaster, GI21357643, Length=289, Percent_Identity=27.681660899654, Blast_Score=85, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_HELHP (Q7VJ31)

Other databases:

- EMBL:   AE017125
- RefSeq:   NP_859950.1
- ProteinModelPortal:   Q7VJ31
- SMR:   Q7VJ31
- GeneID:   1493473
- GenomeReviews:   AE017125_GR
- KEGG:   hhe:HH0419
- NMPDR:   fig|235279.1.peg.419
- HOGENOM:   HBG507643
- OMA:   HIAINAR
- ProtClustDB:   PRK12314
- BioCyc:   HHEP235279:HH_0419-MONOMER
- BRENDA:   2.7.2.11
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; SSF53633 Aa_kinase

EC number: =2.7.2.11

Molecular weight: Translated: 29369; Mature: 29369

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKPRIVLKIGSSNLCNGKIIDKTQIKALAQIISELKMRFDVILVSSGAVASGHTTLHID
CCCCEEEEEECCCCCCCCEEECHHHHHHHHHHHHHHHHHEEEEEEECCCEECCCEEEEEE
RNTLQNKQALASIGQPLLMESYREALKDYAIPTAQLLLVWRDFDSRKNTTFAKDTIDTLL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHEEEEEECCCCCCCCCHHHHHHHHHH
AHNALPIINENDTIATDEMVFGDNDRLGAYVTYYFGAKLLIILSDIDGYFDKNPHQYDDA
HCCCCCEECCCCCEECCCEEECCCCCCCCEEEEECCCEEEEEEECCCHHHCCCCCCCCCC
QILPIVHSIPSQALEQTHSPHGDFATGGIVTKLIAADFLLQRKCMMFLSHGRKLDVLRDF
EEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LLHNKQSSGTLFCPADSQGIKTLI
HHCCCCCCCEEECCCCCCCCHHCC
>Mature Secondary Structure
MQKPRIVLKIGSSNLCNGKIIDKTQIKALAQIISELKMRFDVILVSSGAVASGHTTLHID
CCCCEEEEEECCCCCCCCEEECHHHHHHHHHHHHHHHHHEEEEEEECCCEECCCEEEEEE
RNTLQNKQALASIGQPLLMESYREALKDYAIPTAQLLLVWRDFDSRKNTTFAKDTIDTLL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHEEEEEECCCCCCCCCHHHHHHHHHH
AHNALPIINENDTIATDEMVFGDNDRLGAYVTYYFGAKLLIILSDIDGYFDKNPHQYDDA
HCCCCCEECCCCCEECCCEEECCCCCCCCEEEEECCCEEEEEEECCCHHHCCCCCCCCCC
QILPIVHSIPSQALEQTHSPHGDFATGGIVTKLIAADFLLQRKCMMFLSHGRKLDVLRDF
EEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
LLHNKQSSGTLFCPADSQGIKTLI
HHCCCCCCCEEECCCCCCCCHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12810954