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The map label for this gene is cysK_1

Identifier: 32265562

GI number: 32265562

Start: 68643

End: 69584

Strand: Direct

Name: cysK_1

Synonym: HH0063

Alternate gene names: 32265562

Gene position: 68643-69584 (Clockwise)

Preceding gene: 32265561

Following gene: 32265565

Centisome position: 3.82

GC content: 38.22

Gene sequence:

>942_bases
ATGCTCTATCGTTCAAGTCTTGAACTTATAGGGCATACACCACTTTTAGAACTTACTCATTTGAATATTGCAAACAATAA
CCGCATTTTTGCAAAGCTAGAATTCTATAATCCTGCAGGAGGAATAAAAGACAGGGTAGCTCTTTACATCTGCCAAAAGC
TTAAGGAAAAGGGTCTTTTACATAAAGATTCTGTTATTATTGAAGCTACTGCGGGGAATACAGGACTTGGTATCGCCTTT
GTGGCACAACATTTTGGGTGCAAAGCTATTCTTATCGTACCTGATAAATTCTCTATTGAAAAACAGATTCTAATGCGCGC
ACTTGGCGCACAAGTCATCAATACGCCCAAAGAAAAAGGAATGCAAGGTGCTATGGACAGAGCACAAGAAATGTTATCTT
GCACACCTTATGCTTTAAGTCTTAATCAATTTGAGAATCTTGATAATCCACAAGCACATTATCTTACTACAGCTAAAGAA
ATTTATGATGATATGGCAAAAGTGCAAGCAAATAGTGAAAATGGCTCTGACGCACTCATTGATTATCTTGTATGTGGTGC
TGGGAGCGGAGGGAGCTTTAGCGGTATAGTTGGTTATCTTAAAAAGCAAGATTCTCGCATAAAAGGTGTGCTATGCGACC
CTGTAGGCTCTATTATCGGCGGTGGTGAAGAGGGTTGTGCAAATATTGAAGGTATTGGTAATAACTTTATCCCTCATACA
ATGGATATTTCACTCATTGATAGTGTGCAAAAAATCACAGATGAGGAAGCTTATGAGGGTGTAAGAATGCTAGCTAAAAA
TGAGGGAATCTTAGCAGGCATATCTTCTGGAGCGTGTTTGATAGCTTGTCTAAAACTCGCTAATGAAGTGCAAAATAGCC
TGATTGTAACACTTTTTGCAGATGACTTAAGCCGTTATTTTAGTCGCAATTTAATTGATTAA

Upstream 100 bases:

>100_bases
GGTATAAGCGAACATCTTATCCGCCTTTCTGTTGGTATTGAATATGCACAAGATTTACTTGATGATTTAAATCAAGCCAT
TAAAAAAGCAAAGGTATAGT

Downstream 100 bases:

>100_bases
TATTTACCCTTTCCCTCAGCATAGGATAAGATTCTAAGAGAGTATGCAAGACTTTATCATTTATTCCACCTTTTGCATTC
TCTAGCCACAATGCTCCACA

Product: cysteine synthase

Products: NA

Alternate protein names: CSase; O-acetylserine (thiol)-lyase; OAS-TL; O-acetylserine sulfhydrylase [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MLYRSSLELIGHTPLLELTHLNIANNNRIFAKLEFYNPAGGIKDRVALYICQKLKEKGLLHKDSVIIEATAGNTGLGIAF
VAQHFGCKAILIVPDKFSIEKQILMRALGAQVINTPKEKGMQGAMDRAQEMLSCTPYALSLNQFENLDNPQAHYLTTAKE
IYDDMAKVQANSENGSDALIDYLVCGAGSGGSFSGIVGYLKKQDSRIKGVLCDPVGSIIGGGEEGCANIEGIGNNFIPHT
MDISLIDSVQKITDEEAYEGVRMLAKNEGILAGISSGACLIACLKLANEVQNSLIVTLFADDLSRYFSRNLID

Sequences:

>Translated_313_residues
MLYRSSLELIGHTPLLELTHLNIANNNRIFAKLEFYNPAGGIKDRVALYICQKLKEKGLLHKDSVIIEATAGNTGLGIAF
VAQHFGCKAILIVPDKFSIEKQILMRALGAQVINTPKEKGMQGAMDRAQEMLSCTPYALSLNQFENLDNPQAHYLTTAKE
IYDDMAKVQANSENGSDALIDYLVCGAGSGGSFSGIVGYLKKQDSRIKGVLCDPVGSIIGGGEEGCANIEGIGNNFIPHT
MDISLIDSVQKITDEEAYEGVRMLAKNEGILAGISSGACLIACLKLANEVQNSLIVTLFADDLSRYFSRNLID
>Mature_313_residues
MLYRSSLELIGHTPLLELTHLNIANNNRIFAKLEFYNPAGGIKDRVALYICQKLKEKGLLHKDSVIIEATAGNTGLGIAF
VAQHFGCKAILIVPDKFSIEKQILMRALGAQVINTPKEKGMQGAMDRAQEMLSCTPYALSLNQFENLDNPQAHYLTTAKE
IYDDMAKVQANSENGSDALIDYLVCGAGSGGSFSGIVGYLKKQDSRIKGVLCDPVGSIIGGGEEGCANIEGIGNNFIPHT
MDISLIDSVQKITDEEAYEGVRMLAKNEGILAGISSGACLIACLKLANEVQNSLIVTLFADDLSRYFSRNLID

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=321, Percent_Identity=34.5794392523364, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI295821200, Length=321, Percent_Identity=34.5794392523364, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI4557415, Length=321, Percent_Identity=34.5794392523364, Blast_Score=174, Evalue=1e-43,
Organism=Escherichia coli, GI1788754, Length=314, Percent_Identity=38.2165605095541, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI2367138, Length=308, Percent_Identity=35.3896103896104, Blast_Score=177, Evalue=6e-46,
Organism=Caenorhabditis elegans, GI17535051, Length=315, Percent_Identity=34.6031746031746, Blast_Score=180, Evalue=7e-46,
Organism=Caenorhabditis elegans, GI17562970, Length=315, Percent_Identity=32.6984126984127, Blast_Score=179, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI32566674, Length=311, Percent_Identity=32.475884244373, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI115535073, Length=307, Percent_Identity=34.5276872964169, Blast_Score=161, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17561720, Length=315, Percent_Identity=33.968253968254, Blast_Score=160, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI25147552, Length=327, Percent_Identity=35.1681957186544, Blast_Score=148, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17534315, Length=326, Percent_Identity=35.5828220858896, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI32566672, Length=222, Percent_Identity=33.3333333333333, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17561716, Length=116, Percent_Identity=37.9310344827586, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321594, Length=326, Percent_Identity=35.5828220858896, Blast_Score=196, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6321449, Length=336, Percent_Identity=32.7380952380952, Blast_Score=112, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24643623, Length=319, Percent_Identity=34.7962382445141, Blast_Score=160, Evalue=7e-40,
Organism=Drosophila melanogaster, GI20129101, Length=319, Percent_Identity=34.7962382445141, Blast_Score=160, Evalue=7e-40,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 33851; Mature: 33851

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYRSSLELIGHTPLLELTHLNIANNNRIFAKLEFYNPAGGIKDRVALYICQKLKEKGLL
CCCCCCHHHHCCCCEEEEEEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCE
HKDSVIIEATAGNTGLGIAFVAQHFGCKAILIVPDKFSIEKQILMRALGAQVINTPKEKG
ECCCEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHC
MQGAMDRAQEMLSCTPYALSLNQFENLDNPQAHYLTTAKEIYDDMAKVQANSENGSDALI
HHHHHHHHHHHHCCCCCEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
DYLVCGAGSGGSFSGIVGYLKKQDSRIKGVLCDPVGSIIGGGEEGCANIEGIGNNFIPHT
HHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEECCHHHHHCCCCCCCCCCCCCCCCCCCCC
MDISLIDSVQKITDEEAYEGVRMLAKNEGILAGISSGACLIACLKLANEVQNSLIVTLFA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHEEEEEHH
DDLSRYFSRNLID
HHHHHHHHHCCCC
>Mature Secondary Structure
MLYRSSLELIGHTPLLELTHLNIANNNRIFAKLEFYNPAGGIKDRVALYICQKLKEKGLL
CCCCCCHHHHCCCCEEEEEEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCE
HKDSVIIEATAGNTGLGIAFVAQHFGCKAILIVPDKFSIEKQILMRALGAQVINTPKEKG
ECCCEEEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHC
MQGAMDRAQEMLSCTPYALSLNQFENLDNPQAHYLTTAKEIYDDMAKVQANSENGSDALI
HHHHHHHHHHHHCCCCCEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
DYLVCGAGSGGSFSGIVGYLKKQDSRIKGVLCDPVGSIIGGGEEGCANIEGIGNNFIPHT
HHHHCCCCCCCCHHHHHHHHHHCCCCCCEEEECCHHHHHCCCCCCCCCCCCCCCCCCCCC
MDISLIDSVQKITDEEAYEGVRMLAKNEGILAGISSGACLIACLKLANEVQNSLIVTLFA
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHEEEEEHH
DDLSRYFSRNLID
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9252185 [H]