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The map label for this gene is metB

Identifier: 32265561

GI number: 32265561

Start: 67490

End: 68641

Strand: Direct

Name: metB

Synonym: HH0062

Alternate gene names: 32265561

Gene position: 67490-68641 (Clockwise)

Preceding gene: 32265560

Following gene: 32265562

Centisome position: 3.75

GC content: 39.41

Gene sequence:

>1152_bases
ATGAAAAGCACGCTTGATACATTGCTGATACACGGGGGAGCAACCACAGACCCACGAACAGGTGCAGTTAATATCCCTAT
CTATCAAACTTCTACCTATGCCCAAAGTGCGCTCGGAGAACATTTAGGATATGAATACTCACGCACTAAGAATCCAACGC
GAGATGGCATTGAGAGCCTTATTGCCCAATGTGAGGGTGGAAAATTTGGCTTCGCTTTTGCTTCGGGTATGGCAGCTATT
GGCACGATTTTAAGTCTCTTTCAAAGTGGAGATTGCATTATCATTTCAAATAATGTCTATGGTGGCACTTTTAGAATCTT
AGATAAAGTTTTTTCACATTTTAATATTAGCTATAAAATTGTTGATACACGTGATTTGAAAGCATTAGAATCTGCTATTA
CGCCTGAAGTCAAAGCTGTGCTTATTGAAACTCCAGCAAATCCGCTTCTAAGTGTTACGCCTTTAGAGCAGGTTGCTATA
CTTGCGAAAAAAAAAGGTATTTTAAGCATTGTAGATAATACCTTTATGACGCCTTATTTACAAAAACCTTTAGAGCTTGG
CATTGATATCGTGATGCATTCAGCAACAAAATATCTTGGAGGACATAGCGACCTTATAGCAGGACTCGTAGTTGTAAATG
ATTCTGCACTTGCAGAACGTATCGGCTTTTTACAAAATAGTATTGGGGGAGTGTTAGCACCTTTTGATAGCTTTTTGCTT
ATTCGCGGTATGAAAACACTCGGGGTGCGTCTCCAGCGCCATTGTGAAAATGCTCTCTTTTTAGCACAAGCACTTAGCGA
GCATAGTGGGGTTGAAAAGGTATATTATCCCGGACTTAAAAGTGATGAAGGCTATCAAATACAAAACTCACAGGCTCGTA
GCGGTGGAGGAATGCTAAGTTTTGAACTCAAAAAAAATTATGATTATAGAATCTTTTTTAAATCCACGCAAACAATCGTT
TTAGCCGAAAGTTTAGGAGGTGTAGAATCCTTGCTTTGCCACCCTGCATCTATGACTCATGCTTCAATCCCCAAAGATGT
GCGTGAGCGTATGGGTATAAGCGAACATCTTATCCGCCTTTCTGTTGGTATTGAATATGCACAAGATTTACTTGATGATT
TAAATCAAGCCATTAAAAAAGCAAAGGTATAG

Upstream 100 bases:

>100_bases
AGTGCTTCTTCTGATGTAAGCTGGGAACTGCCCTCACCCAAAGAGCAAGAAATCACAGCAAAAATTCAAGTCAAATATAT
TTGTAAATAAAGGATATTGT

Downstream 100 bases:

>100_bases
TATGCTCTATCGTTCAAGTCTTGAACTTATAGGGCATACACCACTTTTAGAACTTACTCATTTGAATATTGCAAACAATA
ACCGCATTTTTGCAAAGCTA

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CBL; Beta-cystathionase; Cysteine lyase [H]

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MKSTLDTLLIHGGATTDPRTGAVNIPIYQTSTYAQSALGEHLGYEYSRTKNPTRDGIESLIAQCEGGKFGFAFASGMAAI
GTILSLFQSGDCIIISNNVYGGTFRILDKVFSHFNISYKIVDTRDLKALESAITPEVKAVLIETPANPLLSVTPLEQVAI
LAKKKGILSIVDNTFMTPYLQKPLELGIDIVMHSATKYLGGHSDLIAGLVVVNDSALAERIGFLQNSIGGVLAPFDSFLL
IRGMKTLGVRLQRHCENALFLAQALSEHSGVEKVYYPGLKSDEGYQIQNSQARSGGGMLSFELKKNYDYRIFFKSTQTIV
LAESLGGVESLLCHPASMTHASIPKDVRERMGISEHLIRLSVGIEYAQDLLDDLNQAIKKAKV

Sequences:

>Translated_383_residues
MKSTLDTLLIHGGATTDPRTGAVNIPIYQTSTYAQSALGEHLGYEYSRTKNPTRDGIESLIAQCEGGKFGFAFASGMAAI
GTILSLFQSGDCIIISNNVYGGTFRILDKVFSHFNISYKIVDTRDLKALESAITPEVKAVLIETPANPLLSVTPLEQVAI
LAKKKGILSIVDNTFMTPYLQKPLELGIDIVMHSATKYLGGHSDLIAGLVVVNDSALAERIGFLQNSIGGVLAPFDSFLL
IRGMKTLGVRLQRHCENALFLAQALSEHSGVEKVYYPGLKSDEGYQIQNSQARSGGGMLSFELKKNYDYRIFFKSTQTIV
LAESLGGVESLLCHPASMTHASIPKDVRERMGISEHLIRLSVGIEYAQDLLDDLNQAIKKAKV
>Mature_383_residues
MKSTLDTLLIHGGATTDPRTGAVNIPIYQTSTYAQSALGEHLGYEYSRTKNPTRDGIESLIAQCEGGKFGFAFASGMAAI
GTILSLFQSGDCIIISNNVYGGTFRILDKVFSHFNISYKIVDTRDLKALESAITPEVKAVLIETPANPLLSVTPLEQVAI
LAKKKGILSIVDNTFMTPYLQKPLELGIDIVMHSATKYLGGHSDLIAGLVVVNDSALAERIGFLQNSIGGVLAPFDSFLL
IRGMKTLGVRLQRHCENALFLAQALSEHSGVEKVYYPGLKSDEGYQIQNSQARSGGGMLSFELKKNYDYRIFFKSTQTIV
LAESLGGVESLLCHPASMTHASIPKDVRERMGISEHLIRLSVGIEYAQDLLDDLNQAIKKAKV

Specific function: The enzymatic degradation of amino acids in cheese is believed to generate aroma compounds and therefore to be essential for flavor development. Cystathionine beta-lyase (CBL) can convert cystathionine to homocysteine but is also able to catalyze an alpha

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=378, Percent_Identity=48.6772486772487, Blast_Score=325, Evalue=4e-89,
Organism=Homo sapiens, GI299473759, Length=378, Percent_Identity=44.1798941798942, Blast_Score=275, Evalue=5e-74,
Organism=Homo sapiens, GI25453487, Length=377, Percent_Identity=42.7055702917772, Blast_Score=263, Evalue=2e-70,
Organism=Escherichia coli, GI1790375, Length=378, Percent_Identity=39.4179894179894, Blast_Score=286, Evalue=1e-78,
Organism=Escherichia coli, GI1789383, Length=398, Percent_Identity=28.1407035175879, Blast_Score=144, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI17560056, Length=360, Percent_Identity=45, Blast_Score=302, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI17538055, Length=360, Percent_Identity=43.8888888888889, Blast_Score=293, Evalue=1e-79,
Organism=Caenorhabditis elegans, GI71980708, Length=378, Percent_Identity=36.2433862433862, Blast_Score=236, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6319307, Length=385, Percent_Identity=43.3766233766234, Blast_Score=293, Evalue=4e-80,
Organism=Saccharomyces cerevisiae, GI6321254, Length=405, Percent_Identity=33.5802469135802, Blast_Score=195, Evalue=9e-51,
Organism=Saccharomyces cerevisiae, GI6323333, Length=436, Percent_Identity=32.3394495412844, Blast_Score=165, Evalue=9e-42,
Organism=Saccharomyces cerevisiae, GI6321170, Length=299, Percent_Identity=30.7692307692308, Blast_Score=140, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6321904, Length=393, Percent_Identity=29.7709923664122, Blast_Score=137, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6322970, Length=349, Percent_Identity=26.0744985673352, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323557, Length=344, Percent_Identity=26.7441860465116, Blast_Score=77, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6322590, Length=280, Percent_Identity=26.4285714285714, Blast_Score=73, Evalue=9e-14,
Organism=Drosophila melanogaster, GI19922554, Length=376, Percent_Identity=43.3510638297872, Blast_Score=312, Evalue=2e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.8 [H]

Molecular weight: Translated: 41650; Mature: 41650

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSTLDTLLIHGGATTDPRTGAVNIPIYQTSTYAQSALGEHLGYEYSRTKNPTRDGIESL
CCCCHHHHEEECCCCCCCCCCEEEEEEEECHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
IAQCEGGKFGFAFASGMAAIGTILSLFQSGDCIIISNNVYGGTFRILDKVFSHFNISYKI
HHHCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEE
VDTRDLKALESAITPEVKAVLIETPANPLLSVTPLEQVAILAKKKGILSIVDNTFMTPYL
ECCHHHHHHHHHCCCCEEEEEEECCCCCCEEECCHHHHHHHHHCCCHHHHHHHHHCCHHH
QKPLELGIDIVMHSATKYLGGHSDLIAGLVVVNDSALAERIGFLQNSIGGVLAPFDSFLL
HCHHHHHHHHHHHHHHHHHCCCHHHHEEEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHH
IRGMKTLGVRLQRHCENALFLAQALSEHSGVEKVYYPGLKSDEGYQIQNSQARSGGGMLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEECCCCCCCCCCEEE
FELKKNYDYRIFFKSTQTIVLAESLGGVESLLCHPASMTHASIPKDVRERMGISEHLIRL
EEEECCCCEEEEEECCCEEEEEHHCCCHHHHHHCCCCCCCCCCCHHHHHHCCHHHHHHHH
SVGIEYAQDLLDDLNQAIKKAKV
HHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKSTLDTLLIHGGATTDPRTGAVNIPIYQTSTYAQSALGEHLGYEYSRTKNPTRDGIESL
CCCCHHHHEEECCCCCCCCCCEEEEEEEECHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
IAQCEGGKFGFAFASGMAAIGTILSLFQSGDCIIISNNVYGGTFRILDKVFSHFNISYKI
HHHCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEE
VDTRDLKALESAITPEVKAVLIETPANPLLSVTPLEQVAILAKKKGILSIVDNTFMTPYL
ECCHHHHHHHHHCCCCEEEEEEECCCCCCEEECCHHHHHHHHHCCCHHHHHHHHHCCHHH
QKPLELGIDIVMHSATKYLGGHSDLIAGLVVVNDSALAERIGFLQNSIGGVLAPFDSFLL
HCHHHHHHHHHHHHHHHHHCCCHHHHEEEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHH
IRGMKTLGVRLQRHCENALFLAQALSEHSGVEKVYYPGLKSDEGYQIQNSQARSGGGMLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEECCCCCCCCCCEEE
FELKKNYDYRIFFKSTQTIVLAESLGGVESLLCHPASMTHASIPKDVRERMGISEHLIRL
EEEECCCCEEEEEECCCEEEEEHHCCCHHHHHHCCCCCCCCCCCHHHHHHCCHHHHHHHH
SVGIEYAQDLLDDLNQAIKKAKV
HHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]