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The map label for this gene is hyaB

Identifier: 32265556

GI number: 32265556

Start: 61814

End: 63544

Strand: Direct

Name: hyaB

Synonym: HH0057

Alternate gene names: 32265556

Gene position: 61814-63544 (Clockwise)

Preceding gene: 32265555

Following gene: 32265557

Centisome position: 3.44

GC content: 38.71

Gene sequence:

>1731_bases
ATGACAAAACGTATTATTGTAGATCCAATTACAAGAATTGAAGGGCACTTACGAATTGAGGTTATTGTTGATGAAAATAA
TGTGATACAAGATGCGTTCTCAACTTCAACATTATGGCGTGGTTTAGAGACAATCGTTAAAAATCGCGATCCACGTGATG
CAGGTTTTATCGTTCAAAGAATCTGCGGTGTATGTACTTATTCCCATTACAAAGCAGGAATTACTGCTGTAGAAAATGCG
CTAGGGATTAAGCCTCCACTCAATGCTATCCTTACACGCACTTTAATGAATATTTCTCTTTTTTTACACGACCACGCTGT
GCATTTTTATACCTTGCACGGACTTGATTGGTGTGATATTACTTCTGCATTAAAAGCTGATTGCGCTGCTGCTGCAAAAC
TTGCTTTCAAATATAGCCCAAATCCTATCTGCACTGGTGAAAATGAACTCAAAGCTGTGCAAGAAAAAGTAAGCACTTTT
GTTAAACAAGGTGCGCTTGGACCATTTGCTAATGCGTATTGGGGACACAAAACTTATCACTTTACTCCAGAGCAAAATCT
CATTGTTCTCTCGCATTATCTTAAACTCCTTGAAGTGCAACGTGTCATTGCACAAATGATGGCAATTTTTGGTGCAAAAA
ATCCACATCCACAAAGTCTCACTGTAGGTGGCGTAACTTCTGTTATGGATATTTTAGACCCAAGCCGTTTAGGCGAATGG
CTTACTAAGTATAAAGAAGTTACTGAATTTATCAATCGCGCATATTGGGCAGATATTATTATGGCAGCCGAAGCTTACAA
AAATGAAGATTCTGTATTAAAAGGTTGTGGAGTTAAAAACTTTATCAGCTTCCAAGAAATGCAAGTAGGACCTGAAGAGT
GGCTTTTTAGTAGTGGAATCATAAAAGATGGTGATTTAAGTAAAGTATATCCTATTGATGAGAGCAAAATCACAGAAGAA
GCTATACATTCGTGGTATGAGGATAATCAGCCACTCCACCCCTATGATGGCAAAACAAATCCTCAATACACAGGCTTTAA
AGACGCCCAAACTATTGGACCTGATGGTAAGCTTATAGATTCTAAAGTTATTGACGAAAATGGCAAATATTCGTGGATTA
AATCTCCTCGTTATGATGGCGAACCTATGGAAGTTGGACCGCTTGCAAGTGTGCTAGTAGGTTATGCAAGTGGAAACAAA
GCTATTGTTGATATTGTAAATGAACTTCTTAGCGTTACGGGCTTACCTAAAGAAGCTCTTTTTAGCACTCTTGGTAGAAC
TGCAGCACGTGCTATTGAATGTAAAGTTGTCGCAGACCACGGCATAAAAGCTTTCAATGCACTTGTTGCCAATATTGCCT
CTGGAGATAAAAATACTTGTGCACCTTATAGTATTGATAAAAACAAAGAATACAAAGGACGCTTTATCGGTAATGTGCCT
CGAGGAATGCTTAGCCACTGGGTGCGTATAAAAAATGGTGTTATTGAAAACTATCAAGCTGTTGTGCCTTCAACTTGGAA
TGCAGGACCACGTGATGCTAATGGTAAAAAAGGACCCTATGAAATGAGCCTTATTGGCACACAAGTCAAAGATATTACTC
AACCGCTAGAGATTATTCGTCATATTCATTCATTTGACCCGTGTATTGCCTGCGCAGTGCATGTTATGGACACAAAAGGC
AATGAACTAAGTCAATATAAGGTAGAGCCATCATTTGCAAGACTACGATAA

Upstream 100 bases:

>100_bases
GCAGATACAATCGGTGCTGTAGTGCTTGGGGCTGCAGCCATAGGTATTGCCGCACACGCAATTATTAGTTCAACACAAAA
TGCAAAATAAGGGGTAGAAT

Downstream 100 bases:

>100_bases
AGGAGGCGATATGAATACAAATAAAATTGAAATGTTTAAAAAAGAGCTTCATATGCACGAAGAGTTTTCTGGGCTCACGC
GCATATTTCACTGGCTAAGA

Product: Ni/Fe hydrogenase alpha subunit

Products: NA

Alternate protein names: Hydrogen:quinone oxidoreductase; Membrane-bound hydrogenase large subunit [H]

Number of amino acids: Translated: 576; Mature: 575

Protein sequence:

>576_residues
MTKRIIVDPITRIEGHLRIEVIVDENNVIQDAFSTSTLWRGLETIVKNRDPRDAGFIVQRICGVCTYSHYKAGITAVENA
LGIKPPLNAILTRTLMNISLFLHDHAVHFYTLHGLDWCDITSALKADCAAAAKLAFKYSPNPICTGENELKAVQEKVSTF
VKQGALGPFANAYWGHKTYHFTPEQNLIVLSHYLKLLEVQRVIAQMMAIFGAKNPHPQSLTVGGVTSVMDILDPSRLGEW
LTKYKEVTEFINRAYWADIIMAAEAYKNEDSVLKGCGVKNFISFQEMQVGPEEWLFSSGIIKDGDLSKVYPIDESKITEE
AIHSWYEDNQPLHPYDGKTNPQYTGFKDAQTIGPDGKLIDSKVIDENGKYSWIKSPRYDGEPMEVGPLASVLVGYASGNK
AIVDIVNELLSVTGLPKEALFSTLGRTAARAIECKVVADHGIKAFNALVANIASGDKNTCAPYSIDKNKEYKGRFIGNVP
RGMLSHWVRIKNGVIENYQAVVPSTWNAGPRDANGKKGPYEMSLIGTQVKDITQPLEIIRHIHSFDPCIACAVHVMDTKG
NELSQYKVEPSFARLR

Sequences:

>Translated_576_residues
MTKRIIVDPITRIEGHLRIEVIVDENNVIQDAFSTSTLWRGLETIVKNRDPRDAGFIVQRICGVCTYSHYKAGITAVENA
LGIKPPLNAILTRTLMNISLFLHDHAVHFYTLHGLDWCDITSALKADCAAAAKLAFKYSPNPICTGENELKAVQEKVSTF
VKQGALGPFANAYWGHKTYHFTPEQNLIVLSHYLKLLEVQRVIAQMMAIFGAKNPHPQSLTVGGVTSVMDILDPSRLGEW
LTKYKEVTEFINRAYWADIIMAAEAYKNEDSVLKGCGVKNFISFQEMQVGPEEWLFSSGIIKDGDLSKVYPIDESKITEE
AIHSWYEDNQPLHPYDGKTNPQYTGFKDAQTIGPDGKLIDSKVIDENGKYSWIKSPRYDGEPMEVGPLASVLVGYASGNK
AIVDIVNELLSVTGLPKEALFSTLGRTAARAIECKVVADHGIKAFNALVANIASGDKNTCAPYSIDKNKEYKGRFIGNVP
RGMLSHWVRIKNGVIENYQAVVPSTWNAGPRDANGKKGPYEMSLIGTQVKDITQPLEIIRHIHSFDPCIACAVHVMDTKG
NELSQYKVEPSFARLR
>Mature_575_residues
TKRIIVDPITRIEGHLRIEVIVDENNVIQDAFSTSTLWRGLETIVKNRDPRDAGFIVQRICGVCTYSHYKAGITAVENAL
GIKPPLNAILTRTLMNISLFLHDHAVHFYTLHGLDWCDITSALKADCAAAAKLAFKYSPNPICTGENELKAVQEKVSTFV
KQGALGPFANAYWGHKTYHFTPEQNLIVLSHYLKLLEVQRVIAQMMAIFGAKNPHPQSLTVGGVTSVMDILDPSRLGEWL
TKYKEVTEFINRAYWADIIMAAEAYKNEDSVLKGCGVKNFISFQEMQVGPEEWLFSSGIIKDGDLSKVYPIDESKITEEA
IHSWYEDNQPLHPYDGKTNPQYTGFKDAQTIGPDGKLIDSKVIDENGKYSWIKSPRYDGEPMEVGPLASVLVGYASGNKA
IVDIVNELLSVTGLPKEALFSTLGRTAARAIECKVVADHGIKAFNALVANIASGDKNTCAPYSIDKNKEYKGRFIGNVPR
GMLSHWVRIKNGVIENYQAVVPSTWNAGPRDANGKKGPYEMSLIGTQVKDITQPLEIIRHIHSFDPCIACAVHVMDTKGN
ELSQYKVEPSFARLR

Specific function: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789368, Length=588, Percent_Identity=43.7074829931973, Blast_Score=468, Evalue=1e-133,
Organism=Escherichia coli, GI1787207, Length=589, Percent_Identity=39.3887945670628, Blast_Score=416, Evalue=1e-117,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.5.1 [H]

Molecular weight: Translated: 63962; Mature: 63831

Theoretical pI: Translated: 7.10; Mature: 7.10

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRIIVDPITRIEGHLRIEVIVDENNVIQDAFSTSTLWRGLETIVKNRDPRDAGFIVQR
CCCCEEECCHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
ICGVCTYSHYKAGITAVENALGIKPPLNAILTRTLMNISLFLHDHAVHFYTLHGLDWCDI
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEEECEEEEEEECCCCHHHH
TSALKADCAAAAKLAFKYSPNPICTGENELKAVQEKVSTFVKQGALGPFANAYWGHKTYH
HHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCEEE
FTPEQNLIVLSHYLKLLEVQRVIAQMMAIFGAKNPHPQSLTVGGVTSVMDILDPSRLGEW
ECCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCHHHHHHHHCCHHHHHHH
LTKYKEVTEFINRAYWADIIMAAEAYKNEDSVLKGCGVKNFISFQEMQVGPEEWLFSSGI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHCCCHHHHHHHCCC
IKDGDLSKVYPIDESKITEEAIHSWYEDNQPLHPYDGKTNPQYTGFKDAQTIGPDGKLID
CCCCCCCEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEC
SKVIDENGKYSWIKSPRYDGEPMEVGPLASVLVGYASGNKAIVDIVNELLSVTGLPKEAL
HHHHCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHH
FSTLGRTAARAIECKVVADHGIKAFNALVANIASGDKNTCAPYSIDKNKEYKGRFIGNVP
HHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RGMLSHWVRIKNGVIENYQAVVPSTWNAGPRDANGKKGPYEMSLIGTQVKDITQPLEIIR
HHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHH
HIHSFDPCIACAVHVMDTKGNELSQYKVEPSFARLR
HHHCCCHHHHHHHHHCCCCCCCCCEEECCCHHHHCC
>Mature Secondary Structure 
TKRIIVDPITRIEGHLRIEVIVDENNVIQDAFSTSTLWRGLETIVKNRDPRDAGFIVQR
CCCEEECCHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
ICGVCTYSHYKAGITAVENALGIKPPLNAILTRTLMNISLFLHDHAVHFYTLHGLDWCDI
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEEECEEEEEEECCCCHHHH
TSALKADCAAAAKLAFKYSPNPICTGENELKAVQEKVSTFVKQGALGPFANAYWGHKTYH
HHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHCCCCCCEEE
FTPEQNLIVLSHYLKLLEVQRVIAQMMAIFGAKNPHPQSLTVGGVTSVMDILDPSRLGEW
ECCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCHHHHHHHHCCHHHHHHH
LTKYKEVTEFINRAYWADIIMAAEAYKNEDSVLKGCGVKNFISFQEMQVGPEEWLFSSGI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHCCCHHHHHHHCCC
IKDGDLSKVYPIDESKITEEAIHSWYEDNQPLHPYDGKTNPQYTGFKDAQTIGPDGKLID
CCCCCCCEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEC
SKVIDENGKYSWIKSPRYDGEPMEVGPLASVLVGYASGNKAIVDIVNELLSVTGLPKEAL
HHHHCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHH
FSTLGRTAARAIECKVVADHGIKAFNALVANIASGDKNTCAPYSIDKNKEYKGRFIGNVP
HHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RGMLSHWVRIKNGVIENYQAVVPSTWNAGPRDANGKKGPYEMSLIGTQVKDITQPLEIIR
HHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHH
HIHSFDPCIACAVHVMDTKGNELSQYKVEPSFARLR
HHHCCCHHHHHHHHHCCCCCCCCCEEECCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1587288; 8319698; 14500908 [H]