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The map label for this gene is hyaC

Identifier: 32265557

GI number: 32265557

Start: 63555

End: 64319

Strand: Direct

Name: hyaC

Synonym: HH0058

Alternate gene names: 32265557

Gene position: 63555-64319 (Clockwise)

Preceding gene: 32265556

Following gene: 32265558

Centisome position: 3.53

GC content: 35.03

Gene sequence:

>765_bases
ATGAATACAAATAAAATTGAAATGTTTAAAAAAGAGCTTCATATGCACGAAGAGTTTTCTGGGCTCACGCGCATATTTCA
CTGGCTAAGAGCACTATGTATCTTTACGCTCATTGCTACAGGATTCTATATCGCTTATCCTTTTATTATGTCAAATCCCG
ATACGACACAAGCTACTTTGCAAGGAAGTATACAAGGGCAAGTTGTTATTGAACAAAGTATGGGTGCGGTAAGCACTTCT
TACTTGCAAGGTTATATCAGAAGCGTGCATTTGATTATGGGTTTTGTGCTTATTGCCATCTCATTTTTTAGAATCTATCT
TTTTTTCTTTGACAAAAAATCTTTGCCTGAAAGAATCTCGCTTAACCAAGCGCTACAACCTAAAATATGGATAGCCCAAA
TTAAAACCTATATGTTTATAGGCAATCACCCTCATATTGATGGAACTTACAATCCTATACAATTTGTAACCTATTTATGC
CTTGGCATACTCGTATTCCTTATATCTCTCACGGGAGTTGTGCTTTATTATAATGTTTATAATAATGGACTTGGAGCAAT
TTTAGAATTTTGCTTTAAATGGGTAGAGGTGCTTTGTGGAGGATTATCAAGTGTGCGGAATCTGCATCATATTTTTACTT
GGGCGATTATCATCTTTATTCCAGTGCATATTTATTTTGCAGTATGGAATTCAATTAAATACCCAAATGGTGGTGTAGAT
GCTATTGTAAGCGGTATACGCTACACTGATGAAGTAAAAGTTTAA

Upstream 100 bases:

>100_bases
TGTATTGCCTGCGCAGTGCATGTTATGGACACAAAAGGCAATGAACTAAGTCAATATAAGGTAGAGCCATCATTTGCAAG
ACTACGATAAAGGAGGCGAT

Downstream 100 bases:

>100_bases
GGCTTGTATGAAAATTTTAGTGCTTGGCATTGGTAATATCCTCTTTGGTGATGAGGGTATTGGGGTGCATTTAGCCAACT
TTCTTAAACTCAATTATCGC

Product: Ni/Fe hydrogenase cytochrome b subunit

Products: oxidized ferredoxin; H2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MNTNKIEMFKKELHMHEEFSGLTRIFHWLRALCIFTLIATGFYIAYPFIMSNPDTTQATLQGSIQGQVVIEQSMGAVSTS
YLQGYIRSVHLIMGFVLIAISFFRIYLFFFDKKSLPERISLNQALQPKIWIAQIKTYMFIGNHPHIDGTYNPIQFVTYLC
LGILVFLISLTGVVLYYNVYNNGLGAILEFCFKWVEVLCGGLSSVRNLHHIFTWAIIIFIPVHIYFAVWNSIKYPNGGVD
AIVSGIRYTDEVKV

Sequences:

>Translated_254_residues
MNTNKIEMFKKELHMHEEFSGLTRIFHWLRALCIFTLIATGFYIAYPFIMSNPDTTQATLQGSIQGQVVIEQSMGAVSTS
YLQGYIRSVHLIMGFVLIAISFFRIYLFFFDKKSLPERISLNQALQPKIWIAQIKTYMFIGNHPHIDGTYNPIQFVTYLC
LGILVFLISLTGVVLYYNVYNNGLGAILEFCFKWVEVLCGGLSSVRNLHHIFTWAIIIFIPVHIYFAVWNSIKYPNGGVD
AIVSGIRYTDEVKV
>Mature_254_residues
MNTNKIEMFKKELHMHEEFSGLTRIFHWLRALCIFTLIATGFYIAYPFIMSNPDTTQATLQGSIQGQVVIEQSMGAVSTS
YLQGYIRSVHLIMGFVLIAISFFRIYLFFFDKKSLPERISLNQALQPKIWIAQIKTYMFIGNHPHIDGTYNPIQFVTYLC
LGILVFLISLTGVVLYYNVYNNGLGAILEFCFKWVEVLCGGLSSVRNLHHIFTWAIIIFIPVHIYFAVWNSIKYPNGGVD
AIVSGIRYTDEVKV

Specific function: B-type cytochrome that interacts with quinones and anchor the hydrogenase to the membrane [H]

COG id: COG1969

COG function: function code C; Ni,Fe-hydrogenase I cytochrome b subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hupC/hyaC/hydC family [H]

Homologues:

Organism=Escherichia coli, GI1787208, Length=235, Percent_Identity=22.9787234042553, Blast_Score=68, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR000516 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: NA

Molecular weight: Translated: 29105; Mature: 29105

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHCCCCCCCCC
>Mature Secondary Structure
MNTNKIEMFKKELHMHEEFSGLTRIFHWLRALCIFTLIATGFYIAYPFIMSNPDTTQATL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
QGSIQGQVVIEQSMGAVSTSYLQGYIRSVHLIMGFVLIAISFFRIYLFFFDKKSLPERIS
HCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHC
LNQALQPKIWIAQIKTYMFIGNHPHIDGTYNPIQFVTYLCLGILVFLISLTGVVLYYNVY
CCHHCCCHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NNGLGAILEFCFKWVEVLCGGLSSVRNLHHIFTWAIIIFIPVHIYFAVWNSIKYPNGGVD
HCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
AIVSGIRYTDEVKV
HHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced ferredoxin; 2 H+ [C]

Specific reaction: Reduced ferredoxin + 2 H+ = 2 oxidized ferredoxin + H2 [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1587288; 8319698; 14500908 [H]