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The map label for this gene is rho

Identifier: 32265522

GI number: 32265522

Start: 24450

End: 25742

Strand: Direct

Name: rho

Synonym: HH0023

Alternate gene names: 32265522

Gene position: 24450-25742 (Clockwise)

Preceding gene: 32265521

Following gene: 32265523

Centisome position: 1.36

GC content: 39.06

Gene sequence:

>1293_bases
ATGGCAGACAATAAACGAACTCATACACCTGTGGAAGGCTATCAGCTTGAAGATTTGCGTATTAAAAGCATTAAACAACT
CATCAATATTGCTGAAGAAATCGGAGTAGAAAATCCAAGTGACTATCGCCGACAAGATTTAATTTTTGAGATTCTCAAAA
CTCAAGTTAATCAGGGTGGATACATTCTTTTTTCGGGTATATTAGATATCACAAATGAAGGCTATGGCTTCTTACGCGCA
TTAACCGAAAGTCTTGCAGATTCTCAAAATGACACTTATGTATCACAATCTCAAATTCGCCGTTTTGCTTTGCGTAATGG
TGATATAGTAACAGGGCAAGTGCGTGCACCAAAAGACCAAGAGCGATATTATGCTCTCCTTAAAATTGAAGCAATTAACT
ATATGTCGCTTGAAGAAAACAAAAATCGCCCTCTTTTTGATAACCTTACCCCTCTATTCCCACAAGAGCAGCTTAAGCTT
GAATACGAATCTAGCAAGGTTACAGGACGTATGCTTGATCTCTTTAGCCCTATTGGTAAAGGACAACGTGCCCTTATTGT
TGCTCCTCCACGCACAGGAAAAACAGAGTTAATGAAAGAATTGGCTCACGGCATTGCGAAAAATCACCCCGATGTTGAGT
TAATGGTATTGCTTGTAGATGAGCGTCCAGAAGAAGTTACTGATATGGAACGAAGCGTCAAAGGACAAGTTTTTAGCTCT
ACTTTTGATATGCCAGCAACAAACCACATTCGCGTAGCAGATTTAGTCATTGAGAGTGCTAAACGAAAAGTTGAAATGGG
TAAAGATGTAGTAATTCTTCTTGATTCTATCACACGTCTTGCTCGAGCATATAATGCTGCTACTCCCTCAAGTGGTAAAG
TGCTAAGTGGTGGTGTTGATGCAAATGCTTTGCATAAACCTAAACGATTTTTTGGTGCAGCACGTAACATTGAACAAGGT
GGCAGCCTTACTATTATCGCGACTGCACTTATTGAAACAGGCTCAAGAATGGACGAGGTCATCTTTGAGGAATTTAAGGG
CACAGGTAATAGTGAAATTGTCCTTGCACGTTCTATAGCTGACCGCAGAATCTACCCTGCTTTTGATGTGCTTAAAAGTG
GGACACGTAAAGATGATTTGCTTCTTGGCAAAGATAATCTCACAAAAGTATGGATGCTACGCAATGTAATGCACACAATG
GACGATATTGAAGCACTCACTTTCATTTACTCAAAAATGAAACAAACAGAGAATAATGAAGAGTTTTTAAATATGATGAA
TAGTCAAGAATAA

Upstream 100 bases:

>100_bases
GAAGACTCCTTTGGAGTAGGCTTTAGAATCAAATGTAGCTATTGTAGATTCTAAAGACTTCTTGAACTTCTTAACATCTT
CTCTTTGGCTAAGGAATTTT

Downstream 100 bases:

>100_bases
GGATATATATGCGAGCAGGGGTATTTGATAGTGGTGTTGGTGGCTTAAGCGTGCTTAAAAGCCTCATTAATGCAAAACTC
TTTGAGGACATCATTTATTA

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 430; Mature: 429

Protein sequence:

>430_residues
MADNKRTHTPVEGYQLEDLRIKSIKQLINIAEEIGVENPSDYRRQDLIFEILKTQVNQGGYILFSGILDITNEGYGFLRA
LTESLADSQNDTYVSQSQIRRFALRNGDIVTGQVRAPKDQERYYALLKIEAINYMSLEENKNRPLFDNLTPLFPQEQLKL
EYESSKVTGRMLDLFSPIGKGQRALIVAPPRTGKTELMKELAHGIAKNHPDVELMVLLVDERPEEVTDMERSVKGQVFSS
TFDMPATNHIRVADLVIESAKRKVEMGKDVVILLDSITRLARAYNAATPSSGKVLSGGVDANALHKPKRFFGAARNIEQG
GSLTIIATALIETGSRMDEVIFEEFKGTGNSEIVLARSIADRRIYPAFDVLKSGTRKDDLLLGKDNLTKVWMLRNVMHTM
DDIEALTFIYSKMKQTENNEEFLNMMNSQE

Sequences:

>Translated_430_residues
MADNKRTHTPVEGYQLEDLRIKSIKQLINIAEEIGVENPSDYRRQDLIFEILKTQVNQGGYILFSGILDITNEGYGFLRA
LTESLADSQNDTYVSQSQIRRFALRNGDIVTGQVRAPKDQERYYALLKIEAINYMSLEENKNRPLFDNLTPLFPQEQLKL
EYESSKVTGRMLDLFSPIGKGQRALIVAPPRTGKTELMKELAHGIAKNHPDVELMVLLVDERPEEVTDMERSVKGQVFSS
TFDMPATNHIRVADLVIESAKRKVEMGKDVVILLDSITRLARAYNAATPSSGKVLSGGVDANALHKPKRFFGAARNIEQG
GSLTIIATALIETGSRMDEVIFEEFKGTGNSEIVLARSIADRRIYPAFDVLKSGTRKDDLLLGKDNLTKVWMLRNVMHTM
DDIEALTFIYSKMKQTENNEEFLNMMNSQE
>Mature_429_residues
ADNKRTHTPVEGYQLEDLRIKSIKQLINIAEEIGVENPSDYRRQDLIFEILKTQVNQGGYILFSGILDITNEGYGFLRAL
TESLADSQNDTYVSQSQIRRFALRNGDIVTGQVRAPKDQERYYALLKIEAINYMSLEENKNRPLFDNLTPLFPQEQLKLE
YESSKVTGRMLDLFSPIGKGQRALIVAPPRTGKTELMKELAHGIAKNHPDVELMVLLVDERPEEVTDMERSVKGQVFSST
FDMPATNHIRVADLVIESAKRKVEMGKDVVILLDSITRLARAYNAATPSSGKVLSGGVDANALHKPKRFFGAARNIEQGG
SLTIIATALIETGSRMDEVIFEEFKGTGNSEIVLARSIADRRIYPAFDVLKSGTRKDDLLLGKDNLTKVWMLRNVMHTMD
DIEALTFIYSKMKQTENNEEFLNMMNSQE

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=414, Percent_Identity=56.0386473429952, Blast_Score=489, Evalue=1e-139,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 48486; Mature: 48355

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADNKRTHTPVEGYQLEDLRIKSIKQLINIAEEIGVENPSDYRRQDLIFEILKTQVNQGG
CCCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
YILFSGILDITNEGYGFLRALTESLADSQNDTYVSQSQIRRFALRNGDIVTGQVRAPKDQ
EEEEECHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCH
ERYYALLKIEAINYMSLEENKNRPLFDNLTPLFPQEQLKLEYESSKVTGRMLDLFSPIGK
HHEEEEEEEEEECEEECCCCCCCCCCCCCCCCCCHHHHEEEECCCHHHHHHHHHHHHCCC
GQRALIVAPPRTGKTELMKELAHGIAKNHPDVELMVLLVDERPEEVTDMERSVKGQVFSS
CCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHCCHHHHC
TFDMPATNHIRVADLVIESAKRKVEMGKDVVILLDSITRLARAYNAATPSSGKVLSGGVD
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCCCCCCEEECCCC
ANALHKPKRFFGAARNIEQGGSLTIIATALIETGSRMDEVIFEEFKGTGNSEIVLARSIA
CHHHHCHHHHHHHHCCCCCCCCEEEEHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEECCC
DRRIYPAFDVLKSGTRKDDLLLGKDNLTKVWMLRNVMHTMDDIEALTFIYSKMKQTENNE
CCCCCCHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
EFLNMMNSQE
HHHHHHCCCC
>Mature Secondary Structure 
ADNKRTHTPVEGYQLEDLRIKSIKQLINIAEEIGVENPSDYRRQDLIFEILKTQVNQGG
CCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
YILFSGILDITNEGYGFLRALTESLADSQNDTYVSQSQIRRFALRNGDIVTGQVRAPKDQ
EEEEECHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCH
ERYYALLKIEAINYMSLEENKNRPLFDNLTPLFPQEQLKLEYESSKVTGRMLDLFSPIGK
HHEEEEEEEEEECEEECCCCCCCCCCCCCCCCCCHHHHEEEECCCHHHHHHHHHHHHCCC
GQRALIVAPPRTGKTELMKELAHGIAKNHPDVELMVLLVDERPEEVTDMERSVKGQVFSS
CCEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHCCHHHHC
TFDMPATNHIRVADLVIESAKRKVEMGKDVVILLDSITRLARAYNAATPSSGKVLSGGVD
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCCCCCCEEECCCC
ANALHKPKRFFGAARNIEQGGSLTIIATALIETGSRMDEVIFEEFKGTGNSEIVLARSIA
CHHHHCHHHHHHHHCCCCCCCCEEEEHHHHHHCCCHHHHHHHHHHCCCCCCEEEEEECCC
DRRIYPAFDVLKSGTRKDDLLLGKDNLTKVWMLRNVMHTMDDIEALTFIYSKMKQTENNE
CCCCCCHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
EFLNMMNSQE
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]