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The map label for this gene is dnaT

Identifier: 30065603

GI number: 30065603

Start: 4559428

End: 4559967

Strand: Reverse

Name: dnaT

Synonym: S4663

Alternate gene names: 30065603

Gene position: 4559967-4559428 (Counterclockwise)

Preceding gene: 229089129

Following gene: 30065602

Centisome position: 99.14

GC content: 56.67

Gene sequence:

>540_bases
ATGTCTTCCAGAGTTTTGACCCCGGACGTCGTTGGTATTGACGCCCTGGTACACGATCACCAAACCGTTCTGGCAAAAGC
TGAAGGCGGTGTGGTTGCCGTATTTGCTAACAATGCCCCGGCGTTTTATGCCGTCACGCCTGCACGCCTGGCTGAACTGC
TGGCGCTGGAAGAAAAGCTAGCGCGTCCGGGAAGCGATGTCGCTCTGGACGATCAACTCTATCAGGAACCGCAAGCCGCT
CCCGTTGCTGTACCCATGGGGAAATTCGCCATGTATCCGGACTGGCAACCCGATGCCGATTTTATCCGCCTGGCGGCGCT
ATGGGGCGTGGCGCTAAGAGAGCCGGTGACCACCGAAGAACTGGCCTCATTCATTGCCTACTGGCAGGCGGAAGGTAAAG
TCTTTCACCATGTGCAGTGGCAACAAAAACTGGCGCGCAGCCTGCAAATCGGTCGTGCCAGCAACGGCGGACTGCCGAAA
CGAGATGTGAATACGGTCAGTGAACCTGACAGCCAAATTCCACCAGGATTCAGAGGGTAA

Upstream 100 bases:

>100_bases
CTCGCTTAAGCACACGGATGAGAGACAGCCTCCTCTCCTCCGTGTGTTACTATAAAAGTTATCTCCCTTCTCGTTCATCG
TTCCATATTTGAGAAACAGT

Downstream 100 bases:

>100_bases
CGATGAAAAACGTTGGCGACCTGATGCAACGCCTGCAAAAAATGATGCCTGCCCATATCAAACCTGCATTCAAAACGGGT
GAAGAGCTACTGGCCTGGCA

Product: primosomal protein DnaI

Products: NA

Alternate protein names: Primosomal protein I

Number of amino acids: Translated: 179; Mature: 178

Protein sequence:

>179_residues
MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYAVTPARLAELLALEEKLARPGSDVALDDQLYQEPQAA
PVAVPMGKFAMYPDWQPDADFIRLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
RDVNTVSEPDSQIPPGFRG

Sequences:

>Translated_179_residues
MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYAVTPARLAELLALEEKLARPGSDVALDDQLYQEPQAA
PVAVPMGKFAMYPDWQPDADFIRLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
RDVNTVSEPDSQIPPGFRG
>Mature_178_residues
SSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYAVTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAP
VAVPMGKFAMYPDWQPDADFIRLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPKR
DVNTVSEPDSQIPPGFRG

Specific function: This protein is required for primosome-dependent normal DNA replication; it is also involved in inducing stable DNA replication during SOS response. It forms, in concert with dnaB protein and other prepriming proteins dnaC, N, N', N'' a prepriming protein

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaT family

Homologues:

Organism=Escherichia coli, GI1790824, Length=179, Percent_Identity=100, Blast_Score=363, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNAT_ECO24 (A7ZVQ2)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001465881.1
- ProteinModelPortal:   A7ZVQ2
- STRING:   A7ZVQ2
- EnsemblBacteria:   EBESCT00000022750
- GeneID:   5589437
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_4955
- eggNOG:   NOG148646
- GeneTree:   EBGT00050000009365
- HOGENOM:   HBG416722
- OMA:   FVAYWQA
- ProtClustDB:   PRK02854
- BioCyc:   ECOL331111:ECE24377A_4955-MONOMER
- HAMAP:   MF_01061
- InterPro:   IPR020917

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 19455; Mature: 19324

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYAVTPARLAELLALEEKL
CCCCCCCCCHHHHHHHHHCCHHEEEECCCCEEEEEECCCCEEEEECHHHHHHHHHHHHHH
ARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFIRLAALWGVALREPVTTEE
CCCCCCCEECHHHHCCCCCCCEEECCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCHHH
LASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPKRDVNTVSEPDSQIPPGFRG
HHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYAVTPARLAELLALEEKL
CCCCCCCCHHHHHHHHHCCHHEEEECCCCEEEEEECCCCEEEEECHHHHHHHHHHHHHH
ARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFIRLAALWGVALREPVTTEE
CCCCCCCEECHHHHCCCCCCCEEECCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCHHH
LASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPKRDVNTVSEPDSQIPPGFRG
HHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA