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The map label for this gene is hpaC

Identifier: 30065586

GI number: 30065586

Start: 4538025

End: 4538537

Strand: Reverse

Name: hpaC

Synonym: S4643

Alternate gene names: 30065586

Gene position: 4538537-4538025 (Counterclockwise)

Preceding gene: 30065587

Following gene: 30065585

Centisome position: 98.68

GC content: 54.19

Gene sequence:

>513_bases
ATGCAATTAGATGAACAACGCCTGCGCTTTCATGACGCGATGGCCAGCCTGTCGGCAGCGGTAAATATTATCACCACCGA
GGGCGACGCCGGACAATGCGGGATTACGGCAACGGCTGTCTGCTCGGTCACGGATACACCACCGTCGCTGATGGTGTGCA
TTAACGCCAACAGTGCGATGAACCCGGTTTTTCAGGGCAACGGCAAGTTGTGCGTCAACGTCCTCAACCATGAGCAGGAA
CTGATGGCGCGCCACTTCGCGGGCATGACAGGCATGGCGATGGAAGAGCGTTTTAGCCTCTCATGCTGGCAAAAAGGTCC
GCTGGCGCAGCCGGTGCTAAAAGGTTCGCTGGCCAGTCTTGAAGGAGAGATCCGCGATGTGCAGGCGATTGGCACACATC
TGGTGTATCTGGTGGAGATTAAAAACATCATCCTCAGTGCAGAAGGTCACGGACTTATTTACTTTAAACGCCGTTTCCAT
CCGGTGATGCTGGAAATGGAAGCTGCGATTTAA

Upstream 100 bases:

>100_bases
CGGAATACGACCAGAACGGCTGGACTGTGCCGCACCTGCACAACAACGACGATATCAACATGCTGGATAAGCTGCTGAAA
TAACGCAGCAGGAGGTTAAG

Downstream 100 bases:

>100_bases
GCTCCAGCCCCTTCACGCATTCGTCGAAGGGGCATTTTTATTGTGATCTCAATCACATATCTACCCGATGAATAATTCTT
GCCCAACCGCTCACCGCCAT

Product: component C of the 4HPA-hydroxylase

Products: 3,4-dihydroxyphenylacetate; NAD+; H2O

Alternate protein names: 4-HPA 3-monooxygenase small component; Flavin:NADH reductase [H]

Number of amino acids: Translated: 170; Mature: 170

Protein sequence:

>170_residues
MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVTDTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQE
LMARHFAGMTGMAMEERFSLSCWQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
PVMLEMEAAI

Sequences:

>Translated_170_residues
MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVTDTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQE
LMARHFAGMTGMAMEERFSLSCWQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
PVMLEMEAAI
>Mature_170_residues
MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVTDTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQE
LMARHFAGMTGMAMEERFSLSCWQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
PVMLEMEAAI

Specific function: Catalyzes the reduction of free flavins (FMN, FAD and riboflavin) by NADH. Subsequently, the reduced flavins diffuse to the large hpaB component or to other electron acceptors such as cytochrome c and Fe(3+) ion [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. HpaC subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=161, Percent_Identity=44.0993788819876, Blast_Score=138, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002563
- InterPro:   IPR011982
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: 1.14.13.3

Molecular weight: Translated: 18503; Mature: 18503

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
5.9 %Met     (Translated Protein)
8.8 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
5.9 %Met     (Mature Protein)
8.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVTDTPPSLMVCINANSAM
CCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCCCEEEEEEEEECCCCCEEEEEECCCCCC
NPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSCWQKGPLAQPVLKGSLASL
CCEEECCCCEEEEEECCHHHHHHHHHCCCCCCHHHHHCCEECCCCCCCCCHHHHHHHHHH
EGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFHPVMLEMEAAI
CCHHHHHHHHHHHHHHEEEEEEHEEECCCCEEEEEECCCCEEEEEEEECC
>Mature Secondary Structure
MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVTDTPPSLMVCINANSAM
CCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCCCEEEEEEEEECCCCCEEEEEECCCCCC
NPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSCWQKGPLAQPVLKGSLASL
CCEEECCCCEEEEEECCHHHHHHHHHCCCCCCHHHHHCCEECCCCCCCCCHHHHHHHHHH
EGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFHPVMLEMEAAI
CCHHHHHHHHHHHHHHEEEEEEHEEECCCCEEEEEECCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-hydroxyphenylacetate; NADH; H+; O2

Specific reaction: 4-hydroxyphenylacetate + NADH + H+ + O2 = 3,4-dihydroxyphenylacetate + NAD+ + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8077235; 8550403; 10633095 [H]