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The map label for this gene is yjfR

Identifier: 30065563

GI number: 30065563

Start: 4514999

End: 4516069

Strand: Reverse

Name: yjfR

Synonym: S4617

Alternate gene names: 30065563

Gene position: 4516069-4514999 (Counterclockwise)

Preceding gene: 30065570

Following gene: 30065562

Centisome position: 98.19

GC content: 51.73

Gene sequence:

>1071_bases
ATGGCGATGAGTAAAGTGAAAAGTATCACCCGAGAATCCTGGATCCTGAGCACTTTCCCGGAGTGGGGTAGCTGGCTGAA
TGAAGAAATTGAACAAGAGCAGGTCGCCCCTGGCACATTTGCGATGTGGTGGCTAGGCTGCACCGGGATCTGGTTGAAAT
CAGAAGGTGGCACCAACGTTTGCGTTGATTTCTGGTGCGGCACTGGCAAACAAAGTCACGGTAACCCGTTAATGAAACAG
GGTCACCAGATGCAGCGCATGGCTGGCGTGAAAAAACTGCAGCCAAACCTACGCACCACCCCGTTTGTTCTTGATCCGTT
TGCGATTCGCCAGATCGACGCGGTACTGGCGACTCACGATCACAACGATCATATCGACGTTAACGTCGCTGCTGCCGTGA
TGCAGAACTGTGCTGATGACGTACCATTCATTGGACCGAAAACCTGCGTTGATTTGTGGATTGGCTGGGGCGTACCGAAA
GAGCGTTGCATCGTGGTCAAACCGGGCGATGTTGTAAAAGTGAAAGACATTGAAATTCATGCGCTTGACGCTTTCGACCG
TACTGCACTGATCACCCTGCCTGCCGATCAAAAAGCGGCTGGCGTACTGCCAGATGGCATGGACGATCGCGCAGTAAACT
ACCTGTTCAAAACCCCTGGCGGTTCCCTATATCACAGTGGCGATTCCCACTACTCTAACTATTATGCGAAGCACGGTAAC
GAACATCAGATCGATGTGGCGTTAGGATCGTATGGTGAGAACCCGCGCGGTATTACCGACAAAATGACCAGCGCCGATAT
GCTGCGTATGGGTGAAGCCCTGAATGCGAAAGTGGTTATCCCGTTCCACCACGACATCTGGTCAAACTTCCAGGCCGATC
CGCAAGAGATCCGCGTGCTGTGGGAGATCAAAAAAGATCGTCTGAAGTATGGCTTCAAGCCGTTTATCTGGCAGGTTGGC
GGCAAATTTACCTGGCCGCTGGATAAAGACAACTTCGAGTACCACTATCCGCGCGGTTTCGATGATTGCTTCACTATTGA
ACCGGATCTGCCGTTCAAGTCATTCCTGTAA

Upstream 100 bases:

>100_bases
CAAAAAGTCAAAAAATGGTCATTACCTTCCTGAAGGAATAGTGTCAGGATTAATCAAAACCAATCACATGTTGATTCGAA
TTGAACTATCATGAAGGTAA

Downstream 100 bases:

>100_bases
TCTGTCGTTTATACCGGATAGCGTGGAGAAGTTCGCGCTATCCGGCGTAGTATTTCCTGGCTATTTCAAATATCATCTAA
AAAAATCAAATTTTATCGGA

Product: putative L-ascorbate 6-phosphate lactonase

Products: NA

Alternate protein names: L-ascorbate utilization protein G [H]

Number of amino acids: Translated: 356; Mature: 355

Protein sequence:

>356_residues
MAMSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLGCTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQ
GHQMQRMAGVKKLQPNLRTTPFVLDPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPK
ERCIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPGGSLYHSGDSHYSNYYAKHGN
EHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKVVIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVG
GKFTWPLDKDNFEYHYPRGFDDCFTIEPDLPFKSFL

Sequences:

>Translated_356_residues
MAMSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLGCTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQ
GHQMQRMAGVKKLQPNLRTTPFVLDPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPK
ERCIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPGGSLYHSGDSHYSNYYAKHGN
EHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKVVIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVG
GKFTWPLDKDNFEYHYPRGFDDCFTIEPDLPFKSFL
>Mature_355_residues
AMSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLGCTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQG
HQMQRMAGVKKLQPNLRTTPFVLDPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKE
RCIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPGGSLYHSGDSHYSNYYAKHGNE
HQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKVVIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGG
KFTWPLDKDNFEYHYPRGFDDCFTIEPDLPFKSFL

Specific function: Probably catalyzes the hydrolysis of L-ascorbate-6-P into 3-keto-L-gulonate-6-P. Is essential for L-ascorbate utilization under anaerobic conditions [H]

COG id: COG2220

COG function: function code R; Predicted Zn-dependent hydrolases of the beta-lactamase fold

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ulaG family [H]

Homologues:

Organism=Escherichia coli, GI87082387, Length=354, Percent_Identity=99.7175141242938, Blast_Score=739, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 40245; Mature: 40114

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLGCTGIWLKSEGGTNV
CCHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEECCCCCEE
CVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVLDPFAIRQIDAVLATHD
EEEEEECCCCCCCCCHHHHCCHHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHEECC
HNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKERCIVVKPGDVVKVKDIEIH
CCCCEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCEEEECCCCEEEEEEEEEE
ALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPGGSLYHSGDSHYSNYYAKHGN
EECCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCEEECCCCHHHHHHHHCCC
EHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKVVIPFHHDIWSNFQADPQEIRVL
CEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECHHHHCCCCCCHHHHHHH
WEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHYPRGFDDCFTIEPDLPFKSFL
EEEHHHHHHCCCCCEEEECCCEEECCCCCCCCCEECCCCCCCCEEECCCCCHHHCC
>Mature Secondary Structure 
AMSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLGCTGIWLKSEGGTNV
CHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEECCCCCEE
CVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVLDPFAIRQIDAVLATHD
EEEEEECCCCCCCCCHHHHCCHHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHHHEECC
HNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKERCIVVKPGDVVKVKDIEIH
CCCCEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCEEEECCCCEEEEEEEEEE
ALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPGGSLYHSGDSHYSNYYAKHGN
EECCCCCEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCEEECCCCHHHHHHHHCCC
EHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKVVIPFHHDIWSNFQADPQEIRVL
CEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECHHHHCCCCCCHHHHHHH
WEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHYPRGFDDCFTIEPDLPFKSFL
EEEHHHHHHCCCCCEEEECCCEEECCCCCCCCCEECCCCCCCCEEECCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA