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The map label for this gene is nirD

Identifier: 30065348

GI number: 30065348

Start: 4306608

End: 4306934

Strand: Reverse

Name: nirD

Synonym: S4378

Alternate gene names: 30065348

Gene position: 4306934-4306608 (Counterclockwise)

Preceding gene: 30065349

Following gene: 30065347

Centisome position: 93.64

GC content: 53.82

Gene sequence:

>327_bases
ATGAGCCAGTGGAAAGACATCTGCAAAATCGATGACATCCTGCCTGAAACCGGCGTCTGCGCACTGTTAGGTGACAAGCA
GGTAGCGATTTTCCGCCCGTATCACAGCGACCAGGTGTTTGCGATCAGCAACATTGATCCGTTCTTCGAGTCCAGCGTGC
TATCACGCGGGCTGATTGCGGAACACCAGGGCGAGCTGTGGGTCGCCAGCCCGCTGAAAAAACAGCGTTTTCGCTTAAGT
GATGGCTTGTGCATGGAAGACGAACAGTTTTCCGTCAAACATTACGAAGCACGAGTGAAAGACGGCGTGGTGCAGCTGCG
CGGTTAA

Upstream 100 bases:

>100_bases
ACAAGCGTGACCCGAACGTGCAGATGGTGCCAGAGCGCGAACAGCACCGTCCGGCAACGCCGTATGAACGTATCCCGGTA
ACTCTGGTGGAGGACAACGC

Downstream 100 bases:

>100_bases
TGTTTTAACGGGAGGCGCAATGCCTCCCCTTTTTGCATGGTCCTGTAATGACCTTCGGTATATTGCAGGACATTTTTTAA
ACTTTTTGTTTTATTTTTTG

Product: nitrite reductase small subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 108; Mature: 107

Protein sequence:

>108_residues
MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISNIDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLS
DGLCMEDEQFSVKHYEARVKDGVVQLRG

Sequences:

>Translated_108_residues
MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISNIDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLS
DGLCMEDEQFSVKHYEARVKDGVVQLRG
>Mature_107_residues
SQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISNIDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSD
GLCMEDEQFSVKHYEARVKDGVVQLRG

Specific function: Required for activity of the reductase [H]

COG id: COG2146

COG function: function code PR; Ferredoxin subunits of nitrite reductase and ring-hydroxylating dioxygenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To B.subtilis nasE [H]

Homologues:

Organism=Escherichia coli, GI1789766, Length=108, Percent_Identity=99.0740740740741, Blast_Score=223, Evalue=3e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012748
- InterPro:   IPR017881
- InterPro:   IPR017941 [H]

Pfam domain/function: NA

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 12283; Mature: 12152

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISNIDPFFESSVLSRGLIA
CCCCHHHHHHHHCCCCCCEEEEECCCEEEEECCCCCCCEEEECCCCCHHHHHHHHCCCCC
EHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDGVVQLRG
CCCCCEEEECCCCHHHCCCCCCCCCCCCCCCHHHHHHHHCCCEEEECC
>Mature Secondary Structure 
SQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISNIDPFFESSVLSRGLIA
CCCHHHHHHHHCCCCCCEEEEECCCEEEEECCCCCCCEEEECCCCCHHHHHHHHCCCCC
EHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDGVVQLRG
CCCCCEEEECCCCHHHCCCCCCCCCCCCCCCHHHHHHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]