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The map label for this gene is nirC

Identifier: 30065347

GI number: 30065347

Start: 4305676

End: 4306230

Strand: Reverse

Name: nirC

Synonym: S4377

Alternate gene names: 30065347

Gene position: 4306230-4305676 (Counterclockwise)

Preceding gene: 30065348

Following gene: 30065346

Centisome position: 93.63

GC content: 54.77

Gene sequence:

>555_bases
ATGTTCCTCACCTTTGGGGTAAAAGCGGGCAGCATCAGCCACGGGCAAATGTGGGCAATCCTGCCGCAAACCTGGCTGGG
TAACCTGGTCGGTTCCGTCTTCGTTGCCATGCTCTATAGCTGGGGCGGCGGTAGCCTGCTGCCGGTAGATACCAGCATCG
TTCACTCCGTCGCGCTGGCTAAAACCACTGCACCGGCAATGGTACTGTTCTTCAAAGGTGCATTGTGTAACTGGCTGGTT
TGCCTGGCAATCTGGATGGCGCTGCGCACTGAAGGGGCGGCGAAATTTATCGCTATCTGGTGGTGTCTGCTGGCATTTAT
CGCGTCCGGCTACGAGCACTCTATCGCTAACATGACGCTGTTCGCGCTCTCCTGGTTCGGCAACCACAGCGAAGCCTACA
CGCTGGCGGGTATTGGTCATAACCTGCTGTGGGTGACGCTGGGTAATACTTTATCAGGTGCCGTATTCATGGGATTGGGT
TATTGGTATGCTACGCCGAAAGCGAATCGTCCGGTTGCGGACAAATTTAATCAAACTGAAACAGCTGCCGGTTAA

Upstream 100 bases:

>100_bases
GCTCGACCCGTCCGTACGTCCGCTGGTGATGGGCGCGACCTTTGGTATCGCCTTAACGCTGGTGATTATCGCCGGTTCTG
AACTGTTCACCGGACACACC

Downstream 100 bases:

>100_bases
TTACTAAGGGGTTTTTACGTGGATCATTTGCCTATATTTTGCCAATTACGCGATCGCGACTGTCTGATTGTCGGCGGTGG
TGATGTCGCGGAACGCAAAG

Product: nitrite reductase, NirC protein

Products: Proton [Cytoplasm]; nitrite [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 184; Mature: 184

Protein sequence:

>184_residues
MFLTFGVKAGSISHGQMWAILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALCNWLV
CLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYTLAGIGHNLLWVTLGNTLSGAVFMGLG
YWYATPKANRPVADKFNQTETAAG

Sequences:

>Translated_184_residues
MFLTFGVKAGSISHGQMWAILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALCNWLV
CLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYTLAGIGHNLLWVTLGNTLSGAVFMGLG
YWYATPKANRPVADKFNQTETAAG
>Mature_184_residues
MFLTFGVKAGSISHGQMWAILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALCNWLV
CLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYTLAGIGHNLLWVTLGNTLSGAVFMGLG
YWYATPKANRPVADKFNQTETAAG

Specific function: May act as a nitrite transporter [H]

COG id: COG2116

COG function: function code P; Formate/nitrite family of transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FNT transporter (TC 2.A.44) family [H]

Homologues:

Organism=Escherichia coli, GI87082249, Length=184, Percent_Identity=100, Blast_Score=372, Evalue=1e-105,
Organism=Escherichia coli, GI1787132, Length=192, Percent_Identity=30.7291666666667, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000292 [H]

Pfam domain/function: PF01226 Form_Nir_trans [H]

EC number: NA

Molecular weight: Translated: 19870; Mature: 19870

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS01006 FORMATE_NITRITE_TP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLTFGVKAGSISHGQMWAILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALA
CEEEEECCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHH
KTTAPAMVLFFKGALCNWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTL
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
FALSWFGNHSEAYTLAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTE
EEHHHCCCCCCEEEEECCCCCEEEEEECCCHHHHHHHHHHHEEECCCCCCCHHHHCCCCC
TAAG
CCCC
>Mature Secondary Structure
MFLTFGVKAGSISHGQMWAILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALA
CEEEEECCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHH
KTTAPAMVLFFKGALCNWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTL
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
FALSWFGNHSEAYTLAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTE
EEHHHCCCCCCEEEEECCCCCEEEEEECCCHHHHHHHHHHHEEECCCCCCCHHHHCCCCC
TAAG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; nitrite [Periplasm] [C]

Specific reaction: Proton [Periplasm] + nitrite [Periplasm] = Proton [Cytoplasm] + nitrite [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1987123; 11677609 [H]