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The map label for this gene is afaB [H]

Identifier: 30065229

GI number: 30065229

Start: 4166011

End: 4166571

Strand: Direct

Name: afaB [H]

Synonym: S4250

Alternate gene names: 30065229

Gene position: 4166011-4166571 (Clockwise)

Preceding gene: 30065228

Following gene: 30065230

Centisome position: 90.58

GC content: 42.07

Gene sequence:

>561_bases
ATGCTTGTGCAGAGTAAAGTCTTTGAAGAAGATAAAGTAACTGCAGCGCCCTTTATTGTCACTCCGCCACTGTTCAGGCT
TAATCCAGGCCAGCAAAATAAAATGTGGGTTGTGCAGACTGCGCCAGCTTTACAAGACAGGGAAAAATTACACTGGCTAT
GCGCCACCGGCATTACGCCTAAAAAGAGTGATGTTTGGGCCGAGAAAGATAAAGAAAAAATTGCTGAAAATGTGAACCTG
AACATCCAGGTTAACGTACATACCTGCATAAAACTTATTACCAGGCCATCTGGTATTGAAGGCAAGATGGTTAATGCGAG
TGAGAAGTTATCGTGGAAAAAGCAGGGTGATAAAATTGTCGCGGTCAATCAGTCGCCGTTCTACATCAACCTTTCTTCTT
TACAGGTTGGCGGTAGCAAGATTGAGAAACTAGATTATATTGCGCCGTTTTCGCAACATGAGTTTGTTATGCCAAAAACT
AGCGGCCAGGATATTAAATGGTCTGTGATTACTGATGAAGGTGGTGAGAGTCGTTTGTTTATATCTCATTTGCAATCATA
A

Upstream 100 bases:

>100_bases
CACTTCAGTTTTTAAACTGAAGCTTAGTGCAACGCGATTAATCTATGAACAAGGGTCGGATGGCGCAGCGATAACTGTGA
CAAATCCGCAGGATTGGCCG

Downstream 100 bases:

>100_bases
TGTATTACATTTTTATTGAATATTTTTATGCCATGCATATTCCATGTTTTATCGACTGTCATTAATTTTGATAATGGCCT
TACTTGCGGGTCAGTTAAGT

Product: chaperone

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKMWVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNL
NIQVNVHTCIKLITRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFSQHEFVMPKT
SGQDIKWSVITDEGGESRLFISHLQS

Sequences:

>Translated_186_residues
MLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKMWVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNL
NIQVNVHTCIKLITRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFSQHEFVMPKT
SGQDIKWSVITDEGGESRLFISHLQS
>Mature_186_residues
MLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKMWVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNL
NIQVNVHTCIKLITRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFSQHEFVMPKT
SGQDIKWSVITDEGGESRLFISHLQS

Specific function: Involved in the biogenesis of the AFA-III afimbrial adhesin [H]

COG id: COG3121

COG function: function code NU; P pilus assembly protein, chaperone PapD

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI1789532, Length=179, Percent_Identity=30.7262569832402, Blast_Score=95, Evalue=3e-21,
Organism=Escherichia coli, GI1787171, Length=178, Percent_Identity=32.5842696629214, Blast_Score=91, Evalue=4e-20,
Organism=Escherichia coli, GI1790771, Length=178, Percent_Identity=28.6516853932584, Blast_Score=81, Evalue=3e-17,
Organism=Escherichia coli, GI1786743, Length=176, Percent_Identity=29.5454545454545, Blast_Score=71, Evalue=4e-14,
Organism=Escherichia coli, GI1786333, Length=172, Percent_Identity=29.6511627906977, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 20903; Mature: 20903

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKMWVVQTAPALQDREKLHWLCATGITP
CCCCCCCCCCCCCEECCEEECCCEEEECCCCCCCEEEEEECCCCCCHHHEEEEEECCCCC
KKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLITRPSGIEGKMVNASEKLSWKKQGDKIV
CCCCCCCCCHHHHHHHCCCEEEEEEHHHHHHHHCCCCCCCCEEECCHHHCCHHCCCCEEE
AVNQSPFYINLSSLQVGGSKIEKLDYIAPFSQHEFVMPKTSGQDIKWSVITDEGGESRLF
EEECCCEEEEEEEEEECCCHHHHHHEECCCCCCEEECCCCCCCCEEEEEEECCCCCCEEE
ISHLQS
EEECCC
>Mature Secondary Structure
MLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKMWVVQTAPALQDREKLHWLCATGITP
CCCCCCCCCCCCCEECCEEECCCEEEECCCCCCCEEEEEECCCCCCHHHEEEEEECCCCC
KKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLITRPSGIEGKMVNASEKLSWKKQGDKIV
CCCCCCCCCHHHHHHHCCCEEEEEEHHHHHHHHCCCCCCCCEEECCHHHCCHHCCCCEEE
AVNQSPFYINLSSLQVGGSKIEKLDYIAPFSQHEFVMPKTSGQDIKWSVITDEGGESRLF
EEECCCEEEEEEEEEECCCHHHHHHEECCCCCCEEECCCCCCCCEEEEEEECCCCCCEEE
ISHLQS
EEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8002584 [H]