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The map label for this gene is caf1A [H]

Identifier: 30065230

GI number: 30065230

Start: 4166615

End: 4167694

Strand: Direct

Name: caf1A [H]

Synonym: S4254

Alternate gene names: 30065230

Gene position: 4166615-4167694 (Clockwise)

Preceding gene: 30065229

Following gene: 30065233

Centisome position: 90.59

GC content: 43.43

Gene sequence:

>1080_bases
ATGTTTTATCGACTGTCATTAATTTTGATAATGGCCTTACTTGCGGGTCAGTTAAGTGCCCAGGAGTGGTCATTTGATTC
TTCACAGTTGGAAGGAAACGTTAGTGCGGACACTGTGGCAATGTTTAATCAGGGAGAGCAACTCCCCGGAAATTATCGTG
TGGAAATTTATCTTAACGGTGAAAAAGTTGATGTTGGGGAGTTTCCCTTTCATCGTCCGGAATCCCCTGAAGAAAAAGAG
CTTGTTCCATGTTTAACCGTTGATGACCTTATCCACTATGGCATTAAAATTGATAAAAGCAGCAGTGATACTGACAATAA
AAAGAATCAGTGTTTTAAATGGAACTCTATTGAAGGTTTGAAAGTAAATTATGATTTTGATAGCCAGCGCGTACAGATAA
CCGTTCCACAACTGTATCTGCAAGATAAAAAAAGTAGTTTAGCACCAGTGTCGTTATGGAATGAGGGTGTAGCTGCATTC
CGCATGGTGTATCAGACAAATATTGATATTTCCAAACAAAATGATAATCAATCTACCACGCGTAATTCTCGTTATGGCAG
GTTCACTCCAGGGTTTAATCTTGGTGCCTGGAGGTTTCGTAGTTCAGTGACATGGAGCAAGGAACTTGGCCAGTCAGAAA
GATGGCAAAGGGGTTACATGTGGTTTGAGCGAGGCATAAATGCGATTAAAAGCCGATTAACGCTTGGAGAATCTTATACG
TCGTCTGAGGTGTTTGACAGCATACCTTTTCGCGGCGGGATGTTAGCGACCGACGATGCGATGACTCCGCCGGAGGACAG
CTACTACACACCTGTGGTGCATGGAATAGCACAAAGTGAGGCGCAGGTTATTATTAAACAAAATGGGCAAATTATTTTTA
CCCGTTCTGTGCCGCCAGGGCCTTTTGCGCTGGATAATTTACCAACGCTGGCCGTTGGCGGGGAGCTTGATGTTACAGTG
AGAGAGAGTAATGGCGAGGAGCAGTATTTTTCCGTGCCATTTCAAACGCCGGCCATCGCTCTGCATGAAGGATATTTCAA
ATATAGTGTGATGGGGGGGAATATAAAGAAGAAGGTATAA

Upstream 100 bases:

>100_bases
GTGATTACTGATGAAGGTGGTGAGAGTCGTTTGTTTATATCTCATTTGCAATCATAATGTATTACATTTTTATTGAATAT
TTTTATGCCATGCATATTCC

Downstream 100 bases:

>100_bases
AAAGCTCCCCTGTCTCCGTGGGCGAAATCACGGCGATGTACGGGCTTCCTTATGGCGTCACGGTGTATGGAGGGATTCAA
AACGCCACTCATTTTAATGC

Product: fimbrial protein remnant

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQGEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKE
LVPCLTVDDLIHYGIKIDKSSSDTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRGYMWFERGINAIKSRLTLGESYT
SSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGIAQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTV
RESNGEEQYFSVPFQTPAIALHEGYFKYSVMGGNIKKKV

Sequences:

>Translated_359_residues
MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQGEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKE
LVPCLTVDDLIHYGIKIDKSSSDTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRGYMWFERGINAIKSRLTLGESYT
SSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGIAQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTV
RESNGEEQYFSVPFQTPAIALHEGYFKYSVMGGNIKKKV
>Mature_359_residues
MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQGEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKE
LVPCLTVDDLIHYGIKIDKSSSDTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRGYMWFERGINAIKSRLTLGESYT
SSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGIAQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTV
RESNGEEQYFSVPFQTPAIALHEGYFKYSVMGGNIKKKV

Specific function: A probable role in capsular biogenesis. It is likely that the caf1A molecule binds F1 antigen subunits during the extracellular secretion process [H]

COG id: COG3188

COG function: function code NU; P pilus assembly protein, porin PapC

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fimbrial export usher family [H]

Homologues:

Organism=Escherichia coli, GI1790772, Length=365, Percent_Identity=33.4246575342466, Blast_Score=212, Evalue=3e-56,
Organism=Escherichia coli, GI1787172, Length=314, Percent_Identity=33.4394904458599, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1788427, Length=351, Percent_Identity=32.7635327635328, Blast_Score=175, Evalue=4e-45,
Organism=Escherichia coli, GI1786332, Length=369, Percent_Identity=30.6233062330623, Blast_Score=175, Evalue=4e-45,
Organism=Escherichia coli, GI1789533, Length=355, Percent_Identity=32.6760563380282, Blast_Score=174, Evalue=9e-45,
Organism=Escherichia coli, GI1786744, Length=356, Percent_Identity=30.8988764044944, Blast_Score=173, Evalue=1e-44,
Organism=Escherichia coli, GI87081778, Length=355, Percent_Identity=30.7042253521127, Blast_Score=161, Evalue=6e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000015
- InterPro:   IPR018030 [H]

Pfam domain/function: PF00577 Usher [H]

EC number: NA

Molecular weight: Translated: 40535; Mature: 40535

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS01151 FIMBRIAL_USHER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQGEQLPGNYRVEIYLNG
CEEHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCEEEEECCCCCCCCCEEEEEEECC
EKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSSDTDNKKNQCFKWNSIEGL
CEEECCCCCCCCCCCCCHHCCCCEEEHHHHHHCCEEEECCCCCCCCCCCCCEEECCCCCE
KVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAFRMVYQTNIDISKQNDNQSTT
EEEECCCCCEEEEEECCEEEECCCCCCCCHHHHHHHHHHEEEEEECCCEEECCCCCCCCC
RNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRGYMWFERGINAIKSRLTLGESYT
CCCCCCCCCCCCCCCCEEECCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGIAQSEAQVIIKQNGQIIFTRSVPPG
CHHHHHCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHCCCEEEEEECCCCEEEECCCCCC
PFALDNLPTLAVGGELDVTVRESNGEEQYFSVPFQTPAIALHEGYFKYSVMGGNIKKKV
CEECCCCCEEEECCEEEEEEECCCCCCEEEECCCCCCEEEEECCEEEEEEECCCCCCCC
>Mature Secondary Structure
MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQGEQLPGNYRVEIYLNG
CEEHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCEEEEECCCCCCCCCEEEEEEECC
EKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSSDTDNKKNQCFKWNSIEGL
CEEECCCCCCCCCCCCCHHCCCCEEEHHHHHHCCEEEECCCCCCCCCCCCCEEECCCCCE
KVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAFRMVYQTNIDISKQNDNQSTT
EEEECCCCCEEEEEECCEEEECCCCCCCCHHHHHHHHHHEEEEEECCCEEECCCCCCCCC
RNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRGYMWFERGINAIKSRLTLGESYT
CCCCCCCCCCCCCCCCEEECCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGIAQSEAQVIIKQNGQIIFTRSVPPG
CHHHHHCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHCCCEEEEEECCCCEEEECCCCCC
PFALDNLPTLAVGGELDVTVRESNGEEQYFSVPFQTPAIALHEGYFKYSVMGGNIKKKV
CEECCCCCEEEECCEEEEEEECCCCCCEEEECCCCCCEEEEECCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1551441; 9826348; 9748454; 11586360 [H]