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The map label for this gene is arsB

Identifier: 30065213

GI number: 30065213

Start: 4147018

End: 4148307

Strand: Direct

Name: arsB

Synonym: S4233

Alternate gene names: 30065213

Gene position: 4147018-4148307 (Clockwise)

Preceding gene: 30065212

Following gene: 30065214

Centisome position: 90.17

GC content: 54.5

Gene sequence:

>1290_bases
ATGTTACTGGCAGGCGCTATCTTTGTCCTGACCATCGTATTGGTTATCTGGCAGCCGAAAGGTTTAGGCATCGGCTGGAG
TGCAACGCTCGGCGCGGTACTGGCGTTGGTTACGGGCGTGGTTCATCCGGGTGATATTCCGGTGGTGTGGAATATCGTCT
GGAACGCGACGGCGGCGTTTATTGCCGTCATTATCATCAGCCTGCTGCTGGATGAGTCCGGCTTTTTTGAATGGGCAGCG
CTCCACGTTTCACGCTGGGGCAATGGACGCGGTCGCTTGCTGTTTACCTGGATTGTCCTGCTCGGTGCCGCCGTTGCGGC
CCTGTTTGCCAATGATGGCGCGGCGCTTATTTTGACGCCGATTGTCATCGCCATGCTGCTGGCTTTAGGATTCAGTAAAG
GCACGACGCTGGCGTTCGTGATGGCGGCCGGATTCATTGCCGATACCGCCAGCCTGCCGCTTATTGTCTCCAACCTGGTG
AATATCGTTTCCGCTGATTTCTTTGGCCTCGGCTTTCGCGAATACGCCTCGGTGATGGTGCCGGTGGATATCGCCGCGAT
TGTTGCCACGCTGGTGATGCTACATCTCTATTTTCGCAAAGATATTCCGCAGAACTACGATATGGCGCTGCTGAAATCTC
CCGCAGAAGCGATCAAAGATCCTGCTACGTTCAAAACTGGCTGGGTTGTTTTACTGCTTCTGCTGGTGGGATTTTTCGTC
CTGGAACCGCTCGGCATTCCGGTGAGCGCCATTGCAGCTGTGGGCGCACTGATATTATTTGTCGTCGCTAAACGCGGTCA
TGCGATTAATACGGGTAAAGTCCTGCGCGGTGCCCCCTGGCAGATTGTCATCTTCTCGCTCGGCATGTATCTGGTGGTTT
ATGGCCTGCGCAATGCCGGATTAACGGAATATCTTTCTGGCGTACTCAACGTGCTGGCGGATAACGGCCTGTGGGCCGCG
ACGCTCGGCACCGGATTCCTCACCGCCTTCCTCTCTTCTATTATGAACAATATGCCGACGGTACTGGTTGGCGCGTTGTC
CATTGATGGCAGCACGGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAACGTGATTGGCTGCGATTTGGGGCCGA
AAATTACCCCGATTGGTAGCCTGGCAACGCTGCTCTGGCTGCACGTACTTTCGCAGAAGAATATGACTATCAGCTGGGGA
TATTACTTCCGTACAGGGATTATCATGACCCTGCCTGTGCTGTTTGTGACGCTGGCTGCGCTGGCGCTACGTCTCTCTTT
CACTTTGTAA

Upstream 100 bases:

>100_bases
TGGCCAGACAAAACTGTTCCGGAGACAGTAAGAACATTTGCAGTTAAAAATTTAGCTAAACACATATGGATTTGCACATG
TGTTTTATCCGGGAGGCATT

Downstream 100 bases:

>100_bases
TGAGATACTGATATGAGCAACATTACCATTTATCACAACCCGGCCTGCGGCACGTCGCGTAATACGCTGGAGATGATCCG
CAACAGCGGCACAGAACCGA

Product: arsenical pump membrane protein

Products: ADP; phosphate; arsenite [Periplasm] [C]

Alternate protein names: Arsenic efflux pump protein [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPGDIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAA
LHVSRWGNGRGRLLFTWIVLLGAAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAIKDPATFKTGWVVLLLLLVGFFV
LEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLRGAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAA
TLGTGFLTAFLSSIMNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQKNMTISWG
YYFRTGIIMTLPVLFVTLAALALRLSFTL

Sequences:

>Translated_429_residues
MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPGDIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAA
LHVSRWGNGRGRLLFTWIVLLGAAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAIKDPATFKTGWVVLLLLLVGFFV
LEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLRGAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAA
TLGTGFLTAFLSSIMNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQKNMTISWG
YYFRTGIIMTLPVLFVTLAALALRLSFTL
>Mature_429_residues
MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPGDIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAA
LHVSRWGNGRGRLLFTWIVLLGAAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAIKDPATFKTGWVVLLLLLVGFFV
LEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLRGAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAA
TLGTGFLTAFLSSIMNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQKNMTISWG
YYFRTGIIMTLPVLFVTLAALALRLSFTL

Specific function: Involved in arsenical resistance. Thought to form the channel of an arsenite pump [H]

COG id: COG1055

COG function: function code P; Na+/H+ antiporter NhaD and related arsenite permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ArsB family [H]

Homologues:

Organism=Escherichia coli, GI87082277, Length=429, Percent_Identity=100, Blast_Score=830, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000802 [H]

Pfam domain/function: PF02040 ArsB [H]

EC number: NA

Molecular weight: Translated: 45498; Mature: 45498

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPGDIPVVWNIVWNATAAF
CCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHH
IAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLGAAVAALFANDGAALILTP
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
IVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLVNIVSADFFGLGFREYASVMV
HHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
PVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAIKDPATFKTGWVVLLLLLVGFFV
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHH
LEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLRGAPWQIVIFSLGMYLVVYGLRNAG
HCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
LTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSIMNNMPTVLVGALSIDGSTASGVIKEA
HHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCCCHHEEEEEEECCCCHHHHHHHH
MVYANVIGCDLGPKITPIGSLATLLWLHVLSQKNMTISWGYYFRTGIIMTLPVLFVTLAA
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHH
LALRLSFTL
HHHHHHCCC
>Mature Secondary Structure
MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPGDIPVVWNIVWNATAAF
CCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHH
IAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLGAAVAALFANDGAALILTP
HHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
IVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLVNIVSADFFGLGFREYASVMV
HHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
PVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAIKDPATFKTGWVVLLLLLVGFFV
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHHH
LEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLRGAPWQIVIFSLGMYLVVYGLRNAG
HCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
LTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSIMNNMPTVLVGALSIDGSTASGVIKEA
HHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCCCHHEEEEEEECCCCHHHHHHHH
MVYANVIGCDLGPKITPIGSLATLLWLHVLSQKNMTISWGYYFRTGIIMTLPVLFVTLAA
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHH
LALRLSFTL
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arsenite [Cytoplasm]; H2O [C]

Specific reaction: ATP + arsenite [Cytoplasm] + H2O = ADP + phosphate + arsenite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9006011 [H]