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The map label for this gene is yieO

Identifier: 30064940

GI number: 30064940

Start: 3823225

End: 3824628

Strand: Direct

Name: yieO

Synonym: S3930

Alternate gene names: 30064940

Gene position: 3823225-3824628 (Clockwise)

Preceding gene: 30064939

Following gene: 229089133

Centisome position: 83.13

GC content: 49.36

Gene sequence:

>1404_bases
ATGTCGGGTTTGCCGTGGATCGCGGCGATGGCCTTCTTCATGCAGGCACTTGATGCCACTATTCTGAATACCGCCTTACC
CGCAATCGCTCATAGCCTTAATCGTTCTCCTCTCGCGATGCAATCAGCCATCATCAGTTATACGCTGACGGTGGCGATGC
TTATTCCGGTAAGCGGATGGCTAGCCGATCGCTTCGGTACGCGTCGCATTTTTACCCTTGCCGTGAGTCTGTTCACGTTG
GGTTCTCTGGCCTGCGCACTTTCTAATTCGCTACCACAGCTGGTTGTCTTCCGGGTTATTCAAGGGATAGGCGACGCAAT
GATGATGCCTGTTGCTCGGCCGGCCTTACTGCGTGCTTATCCTCGTAATGAACTTCTTCCGGTATTGAATTTTGTCGCCA
TGCCGGGTCTGGTGGGGCCAATTTTAGGCCCCGTTCTTGGCGGCGTGCTGGTCACCTGGGCAACCTGGCACTGGATATTT
TTAATTAATATCCCCATAGGTATTGCGGGCCTTCTTTACGCGCGCAAACATATGCCCAATTTCACCACCGCACGACGCAG
ATTCGATATCACTGGCTTTTTGCTGTTTGGCCTCAGCCTTGTTCTCTTTTCAAGCGGAATAGAGCTATTCGGGGAAAAGA
TTGTCGCCAGCTGGATTGCCTTGACGGTAATTGTCACCAGCATCGGGTTACTGCTTCTCTATATTCTCCATGCGCGACGT
ACGCCAAACCCATTAATTTCATTAGATTTATTTAAAACCCGCACTTTCTCGATCGGTATCGTAGGCAATATTGCAACCCG
TCTGGGGACCGGCTGTGTACCGTTCCTTATGCCATTGATGTTACAGGTAGGATTTGGTTATCAGGCGTTTATTGCCGGCT
GTATGATGGCACCGACAGCGTTAGGTTCCATTATTGCAAAATCGATGGTTACCCAAGTCTTACGTCGTCTGGGCTATCGC
CATACATTAGTGGGGATCACGGTGATTATTGGGCTAATGATCGCTCAGTTCTCTTTGCAATCACCGGCAATGGCGATATG
GATGCTGATCTTGCCGTTGTTTATATTAGGGATGGCTATGTCGACGCAGTTTACCGCGATGAATACCATCACACTTGCCG
ATCTGACCGATGACAACGCCAGCAGCGGTAACAGTGTTCTGGCGGTCACGCAGCAACTGTCGATTAGTTTAGGCGTTGCT
GTAAGTGCGGCCGTCCTTCGCATTTATGAAGGGATGGAAGGCACAACGACTGTCGAACAATTCCACTATACGTTTATCAC
GATGGGCATTATTACTGTTGCTTCAGCAGCAATGTTCATGCTTCTGAAAACAACCGATGGTAATAATTTGATCAAAAGAC
AGCGTAAATCTATGCCGAACCGCGTTCCATCAGAATCGGAGTAA

Upstream 100 bases:

>100_bases
GGCGACGCGGCATTTAAAGCTTGTCAGGCGCTGCTACGCTCACCTGATAAGTGATAACCCGATAACAGGACATTGAATGA
GCGATAAAAAGAAGCGTAGT

Downstream 100 bases:

>100_bases
GTTGTAATCGTTGTTGCTGAAGGGTCGGCTGGGTTATCCGATGGATGAGTACATCAATCGCCAGCTCCCCCAGTTCATCT
TTCGGTTGGTGGATAGTGGT

Product: putative transport protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRSPLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTL
GSLACALSNSLPQLVVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVTWATWHWIF
LINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELFGEKIVASWIALTVIVTSIGLLLLYILHARR
TPNPLISLDLFKTRTFSIGIVGNIATRLGTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYR
HTLVGITVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGNSVLAVTQQLSISLGVA
VSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLLKTTDGNNLIKRQRKSMPNRVPSESE

Sequences:

>Translated_467_residues
MSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRSPLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTL
GSLACALSNSLPQLVVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVTWATWHWIF
LINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELFGEKIVASWIALTVIVTSIGLLLLYILHARR
TPNPLISLDLFKTRTFSIGIVGNIATRLGTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYR
HTLVGITVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGNSVLAVTQQLSISLGVA
VSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLLKTTDGNNLIKRQRKSMPNRVPSESE
>Mature_466_residues
SGLPWIAAMAFFMQALDATILNTALPAIAHSLNRSPLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLG
SLACALSNSLPQLVVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVTWATWHWIFL
INIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELFGEKIVASWIALTVIVTSIGLLLLYILHARRT
PNPLISLDLFKTRTFSIGIVGNIATRLGTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRH
TLVGITVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGNSVLAVTQQLSISLGVAV
SAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLLKTTDGNNLIKRQRKSMPNRVPSESE

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=467, Percent_Identity=98.9293361884368, Blast_Score=921, Evalue=0.0,
Organism=Escherichia coli, GI1788392, Length=459, Percent_Identity=45.0980392156863, Blast_Score=359, Evalue=1e-100,
Organism=Escherichia coli, GI1789042, Length=426, Percent_Identity=32.1596244131455, Blast_Score=168, Evalue=9e-43,
Organism=Escherichia coli, GI1788710, Length=437, Percent_Identity=32.2654462242563, Blast_Score=162, Evalue=6e-41,
Organism=Escherichia coli, GI87081983, Length=413, Percent_Identity=27.6029055690073, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1788509, Length=136, Percent_Identity=29.4117647058824, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322958, Length=473, Percent_Identity=25.5813953488372, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321663, Length=147, Percent_Identity=31.9727891156463, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6325455, Length=127, Percent_Identity=31.496062992126, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6319770, Length=402, Percent_Identity=24.1293532338308, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50576; Mature: 50445

Theoretical pI: Translated: 10.60; Mature: 10.60

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRSPLAMQSAIISYTLTVAMLIPVSGW
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LADRFGTRRIFTLAVSLFTLGSLACALSNSLPQLVVFRVIQGIGDAMMMPVARPALLRAY
HHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHCCHHHHHHC
PRNELLPVLNFVAMPGLVGPILGPVLGGVLVTWATWHWIFLINIPIGIAGLLYARKHMPN
CCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHCCC
FTTARRRFDITGFLLFGLSLVLFSSGIELFGEKIVASWIALTVIVTSIGLLLLYILHARR
HHHHHHHHCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TPNPLISLDLFKTRTFSIGIVGNIATRLGTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTA
CCCCCEEEEEEEEEEEEEEEHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LGSIIAKSMVTQVLRRLGYRHTLVGITVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAM
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
STQFTAMNTITLADLTDDNASSGNSVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQ
HCHHHHEEEEEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
FHYTFITMGIITVASAAMFMLLKTTDGNNLIKRQRKSMPNRVPSESE
HHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SGLPWIAAMAFFMQALDATILNTALPAIAHSLNRSPLAMQSAIISYTLTVAMLIPVSGW
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LADRFGTRRIFTLAVSLFTLGSLACALSNSLPQLVVFRVIQGIGDAMMMPVARPALLRAY
HHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHCCHHHHHHC
PRNELLPVLNFVAMPGLVGPILGPVLGGVLVTWATWHWIFLINIPIGIAGLLYARKHMPN
CCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHCCC
FTTARRRFDITGFLLFGLSLVLFSSGIELFGEKIVASWIALTVIVTSIGLLLLYILHARR
HHHHHHHHCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TPNPLISLDLFKTRTFSIGIVGNIATRLGTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTA
CCCCCEEEEEEEEEEEEEEEHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LGSIIAKSMVTQVLRRLGYRHTLVGITVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAM
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
STQFTAMNTITLADLTDDNASSGNSVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQ
HCHHHHEEEEEEEECCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
FHYTFITMGIITVASAAMFMLLKTTDGNNLIKRQRKSMPNRVPSESE
HHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]