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The map label for this gene is repA [H]

Identifier: 30063896

GI number: 30063896

Start: 2611291

End: 2612118

Strand: Reverse

Name: repA [H]

Synonym: S2721

Alternate gene names: 30063896

Gene position: 2612118-2611291 (Counterclockwise)

Preceding gene: 30063897

Following gene: 30063895

Centisome position: 56.79

GC content: 55.68

Gene sequence:

>828_bases
ATGGCTCTTGATCTTATGGCGGCTTTCACGGAATTGCCGCCACCCATTGATTATGTTCTGCCTAATATGGTTGCTGGCAC
TGTTGGTGCTCTTGTGTCGCCTGGTGGGGCTGGTAAATCCATGTTGGCCCTTCAATTGGCTGCACAGATTGCAGGCGGGC
CTGATTTACTTGAAATAGGCGAGTTTCCCACCGGGCAAGTGGTCTATCTGCCTGCTGAAGATCCACCGGCCGCTATTCAC
CATCGTCTGCACGCCCTTGGGGCACACCTCAGCGCAGCGGAACGGCAAGCCGTGGCTGATGGTTTGCTCATTGAACCCTT
GATCGGTAAATGCCCAAACATCATGGCTGCTAGCTGGTTCGATGCTCTCAAACGAGCCGCTGAAGGTCGTCGCTTGATGA
TCTTGGACACTTTGCGGCGCTTCCACATTGAGGAGGAGAACGCTAGCGGGCCGATGGCGCAGGTTGTTGGGCACATGGAG
GCCATAGCCGCTGATACAGGCTGCTCCATTGTGTTTCTGCACCACGCGAGTAAAAGCGCAGCCATGATGGGGTCGGGCGA
TCAACAGCAGGCCAGTCGTGGATCGTCCGTACTGGTTGATAACATCCGTTGGCAATCGTACCTATCCGGCATGACGCAAG
GCGAGGCCGAGATACTGGGGGTCGATGATTGTCAGCGTGGGTATTTTGTCCGCTTTGGCGTCAGCAAGGCCAACTATGGC
GCACCTTTTCAAGAACTCTGGTTTAGACGGCACGACGGCGGCGTGTTGAAGCCTGCTGTACTGGAGCGGCAGTGCAAGGT
GAAAAGGAGACAGCGTGAAGAAGCCTAA

Upstream 100 bases:

>100_bases
AATGTCGCCTGGGATTTGTCAGAGGTCGAAGAGTGGATTGAGGCACGTAAATCATCGGGTGATCAAGCTGCGCGACCTGG
CCCTATAGAGGGCTAGTTAT

Downstream 100 bases:

>100_bases
GCATGACCTGACCCACGTCCGACATGATCCCGCGCACTGTTTGGCACCTGGCCTGTTCCGCAGCCTCAAGCGTGGCGATC
GCAAACGCTGCAAGCTGGAC

Product: putative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MALDLMAAFTELPPPIDYVLPNMVAGTVGALVSPGGAGKSMLALQLAAQIAGGPDLLEIGEFPTGQVVYLPAEDPPAAIH
HRLHALGAHLSAAERQAVADGLLIEPLIGKCPNIMAASWFDALKRAAEGRRLMILDTLRRFHIEEENASGPMAQVVGHME
AIAADTGCSIVFLHHASKSAAMMGSGDQQQASRGSSVLVDNIRWQSYLSGMTQGEAEILGVDDCQRGYFVRFGVSKANYG
APFQELWFRRHDGGVLKPAVLERQCKVKRRQREEA

Sequences:

>Translated_275_residues
MALDLMAAFTELPPPIDYVLPNMVAGTVGALVSPGGAGKSMLALQLAAQIAGGPDLLEIGEFPTGQVVYLPAEDPPAAIH
HRLHALGAHLSAAERQAVADGLLIEPLIGKCPNIMAASWFDALKRAAEGRRLMILDTLRRFHIEEENASGPMAQVVGHME
AIAADTGCSIVFLHHASKSAAMMGSGDQQQASRGSSVLVDNIRWQSYLSGMTQGEAEILGVDDCQRGYFVRFGVSKANYG
APFQELWFRRHDGGVLKPAVLERQCKVKRRQREEA
>Mature_274_residues
ALDLMAAFTELPPPIDYVLPNMVAGTVGALVSPGGAGKSMLALQLAAQIAGGPDLLEIGEFPTGQVVYLPAEDPPAAIHH
RLHALGAHLSAAERQAVADGLLIEPLIGKCPNIMAASWFDALKRAAEGRRLMILDTLRRFHIEEENASGPMAQVVGHMEA
IAADTGCSIVFLHHASKSAAMMGSGDQQQASRGSSVLVDNIRWQSYLSGMTQGEAEILGVDDCQRGYFVRFGVSKANYGA
PFQELWFRRHDGGVLKPAVLERQCKVKRRQREEA

Specific function: This protein is involved in regulating the plasmid copy- number. Increasing the level of this protein results in a higher plasmid copy-number [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 29578; Mature: 29447

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALDLMAAFTELPPPIDYVLPNMVAGTVGALVSPGGAGKSMLALQLAAQIAGGPDLLEIG
CCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEECC
EFPTGQVVYLPAEDPPAAIHHRLHALGAHLSAAERQAVADGLLIEPLIGKCPNIMAASWF
CCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEECHHHCCCCCHHHHHHH
DALKRAAEGRRLMILDTLRRFHIEEENASGPMAQVVGHMEAIAADTGCSIVFLHHASKSA
HHHHHHHCCCEEEHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCH
AMMGSGDQQQASRGSSVLVDNIRWQSYLSGMTQGEAEILGVDDCQRGYFVRFGVSKANYG
HHCCCCCHHHHHCCCEEEEEHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEECCCCCCC
APFQELWFRRHDGGVLKPAVLERQCKVKRRQREEA
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ALDLMAAFTELPPPIDYVLPNMVAGTVGALVSPGGAGKSMLALQLAAQIAGGPDLLEIG
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEECC
EFPTGQVVYLPAEDPPAAIHHRLHALGAHLSAAERQAVADGLLIEPLIGKCPNIMAASWF
CCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEECHHHCCCCCHHHHHHH
DALKRAAEGRRLMILDTLRRFHIEEENASGPMAQVVGHMEAIAADTGCSIVFLHHASKSA
HHHHHHHCCCEEEHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCH
AMMGSGDQQQASRGSSVLVDNIRWQSYLSGMTQGEAEILGVDDCQRGYFVRFGVSKANYG
HHCCCCCHHHHHCCCEEEEEHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEECCCCCCC
APFQELWFRRHDGGVLKPAVLERQCKVKRRQREEA
CCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2653965 [H]