Click here to switch to the map view.

The map label for this gene is yfbB

Identifier: 30063694

GI number: 30063694

Start: 2369021

End: 2369779

Strand: Reverse

Name: yfbB

Synonym: S2476

Alternate gene names: 30063694

Gene position: 2369779-2369021 (Counterclockwise)

Preceding gene: 30063695

Following gene: 30063693

Centisome position: 51.52

GC content: 56.13

Gene sequence:

>759_bases
ATGATCCTGCACGCGCAGGCAAAACACGGAAAACTAGGTTTACCCTGGCTGGTGTTTTTGCACGGTTTTTCCGGCGATTG
CCACGAATGGCAAGAAGTGGGCGAGGCGTTTGCCGACTACTCACGGTTGTATGTTGATCTCCTAGGTCATGGTGGTTCGG
CGGCGATTAGCGTCGATGGATTTGATGATGTCACCGACTTACTGCGTAAAACCTTGGTTAGTTACAACATCCTTGACTTC
TGGCTGGTGGGGTACTCGCTTGGCGGACGGGTGGCAATGATGGCGGCTTGCCAGGGGCTGGCGGGGCTTTGTGGGGTTAT
TGTCGAAGGCGGGCATCCGGGGCTGCAAAATGCTGAACAACGTGCGGAACGTCAGCGTTCCGATCGCCAATGGGCGCAGC
GTTTTCGCACAGAACCGTTAACGGCGGTATTTGCCGACTGGTATCAACAGCCTGTTTTTGCCTCGCTCAATGACGATCAA
CGCCGGGAGCTGGTGGCGCTGCGCAGCAACAATAATGGCGCAACCCTTGCCGCCATGCTGGAGGCGACTTCTCTCGCCGT
CCAGCCTGATTTACGTGCTAACCTTAGCGCCCGCACATTTGCGTTTTATTATTTATGTGGTGAACGTGACAGCAAATTCC
GCGCCCTGGCGGCGGAACTGGCTGCCGACTGCCATGTCATTCCTCGCGCCGGACATAACGCGCATCGGGAAAATCCCGCT
GGCGTAATCGCAAGTCTGGCGCAGATCTTGCGTTTCTGA

Upstream 100 bases:

>100_bases
ACGCCTGGCGCACGCCAACCACCACGGTGATTGAAATGGTGGTTAACGACACCGATGGTGCGCAAACGCTCCAGCAACTT
CTGGCGCAGGTAAGCCATTT

Downstream 100 bases:

>100_bases
CTAAAGGACACAATATGATTTATCCTGATGAAGCAATGCCTTACGCACCGGTTGAATGGCACGACTGCTCCGAAGGTTTC
GAGGACATTCGTTATGAAAA

Product: acyl-CoA thioester hydrolase YfbB

Products: NA

Alternate protein names: SHCHC synthase [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLYVDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDF
WLVGYSLGGRVAMMAACQGLAGLCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAAELAADCHVIPRAGHNAHRENPA
GVIASLAQILRF

Sequences:

>Translated_252_residues
MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLYVDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDF
WLVGYSLGGRVAMMAACQGLAGLCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAAELAADCHVIPRAGHNAHRENPA
GVIASLAQILRF
>Mature_252_residues
MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLYVDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDF
WLVGYSLGGRVAMMAACQGLAGLCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAAELAADCHVIPRAGHNAHRENPA
GVIASLAQILRF

Specific function: Catalyzes a proton abstraction reaction that results in 2,5-elimination of pyruvate from 2-succinyl-5-enolpyruvyl-6- hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) and the formation of 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC). Is also

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. MenH family [H]

Homologues:

Organism=Escherichia coli, GI1788598, Length=252, Percent_Identity=98.4126984126984, Blast_Score=508, Evalue=1e-145,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR022485 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =4.2.99.20 [H]

Molecular weight: Translated: 27730; Mature: 27730

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLYVDLLGHGGSAAISVDG
CEECCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
FDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAGLCGVIVEGGHPGLQNAEQ
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
RAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQRRELVALRSNNNGATLAAML
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEECCCCHHHHHEEEEECCCCCCHHHHHH
EATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAAELAADCHVIPRAGHNAHRENPA
HHHHHEECCCHHCCCCHHEEEEEEEECCCCCHHHHHHHHHHHCCEEECCCCCCCCCCCHH
GVIASLAQILRF
HHHHHHHHHHCC
>Mature Secondary Structure
MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLYVDLLGHGGSAAISVDG
CEECCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECC
FDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAGLCGVIVEGGHPGLQNAEQ
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
RAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQRRELVALRSNNNGATLAAML
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEECCCCHHHHHEEEEECCCCCCHHHHHH
EATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAAELAADCHVIPRAGHNAHRENPA
HHHHHEECCCHHCCCCHHEEEEEEEECCCCCHHHHHHHHHHHCCEEECCCCCCCCCCCHH
GVIASLAQILRF
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA