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The map label for this gene is hisF

Identifier: 30063475

GI number: 30063475

Start: 2085095

End: 2085871

Strand: Direct

Name: hisF

Synonym: S2208

Alternate gene names: 30063475

Gene position: 2085095-2085871 (Clockwise)

Preceding gene: 30063474

Following gene: 30063476

Centisome position: 45.33

GC content: 51.99

Gene sequence:

>777_bases
ATGCTGGCAAAACGCATAATCCCGTGTCTCGACGTTCGTGATGGTCAGGTGGTGAAAGGCGTACAGTTTCGCAACCATGA
AATCATTGGCGATATCGTGCCACTGGCAAAACGCTACGCCGAAGAAGGTGCAGATGAACTGGTGTTCTACGATATCACCG
CTTCCAGCGATGGCCGTGTGGTAGATAAAAGCTGGGTATCTCGCGTGGCGGAAGTGATCGACATTCCGTTTTGTGTGGCG
GGTGGGATTAAGTCTCTGGAAGATGCCGCGAAAATTCTTTCCTTTGGCGCGGATAAAATTTCCATCAACTCCCCTGCGCT
GGCAGACCCAACATTAATTACTCGCCTGGCCGATCGCTTTGGCGTGCAGTGTATTGTGGTTGGTATTGATACCTGGTACG
ACGCCGAAACCGGTAAATATCATGTAAATCAATATACCGGCGATGAAAGCCGCACCCGCGTCACTCAATGGGAAACGCTC
GACTGGGTAGAGGAGGTGCAAAAACGCGGCGCCGGAGAAATCGTCCTCAACATGATGAATCAGGACGGCGTGTGTAACGG
TTACGACCTCAAGCAGCTGAAAAAAGTGCGTGAAGTTTGCCACGTCCCGCTGATTGCCTCCGGTGGCGCGGGCACCATGG
AACACTTCCTGGAAGCCTTCCGCGATGCCGACGTTGACGGTGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAAT
ATTGGCGAATTAAAAGCGTACCTGGCAACACAGGGCGTGGAGATCAGGATATGTTAA

Upstream 100 bases:

>100_bases
ACATTAACGATGTTGCTGCCCTACGTGGCACTGGCGTGCGCGGCGTAATAGTTGGTCGTGCATTACTGGAAGGTAAATTC
ACCGTGAAGGAGGCCATCGC

Downstream 100 bases:

>100_bases
CAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGTCTGATGCCGGTGATTGTGCAACACGCGGTATCTGGC
GAAGTGTTAATGCTGGGCTA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGADELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVA
GGIKSLEDAAKILSFGADKISINSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLEAFRDADVDGALAASVFHKQIIN
IGELKAYLATQGVEIRIC

Sequences:

>Translated_258_residues
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGADELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVA
GGIKSLEDAAKILSFGADKISINSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLEAFRDADVDGALAASVFHKQIIN
IGELKAYLATQGVEIRIC
>Mature_258_residues
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGADELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVA
GGIKSLEDAAKILSFGADKISINSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLEAFRDADVDGALAASVFHKQIIN
IGELKAYLATQGVEIRIC

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=258, Percent_Identity=98.8372093023256, Blast_Score=521, Evalue=1e-149,
Organism=Escherichia coli, GI87082028, Length=231, Percent_Identity=27.2727272727273, Blast_Score=68, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6319725, Length=318, Percent_Identity=31.4465408805031, Blast_Score=130, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 28402; Mature: 28402

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGADELVFYDITASSDGRV
CCHHHCCCCCCCCCCCEEECEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCE
VDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISINSPALADPTLITRLADRF
ECHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHH
GVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETLDWVEEVQKRGAGEIVLNMMN
CCEEEEEEECCCCCCCCCCEEEEEECCCCHHHEEHHHHHHHHHHHHHHCCCCCEEEEECC
QDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLEAFRDADVDGALAASVFHKQIIN
CCCCCCCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
IGELKAYLATQGVEIRIC
HHHHHHHHHCCCEEEEEC
>Mature Secondary Structure
MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGADELVFYDITASSDGRV
CCHHHCCCCCCCCCCCEEECEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCE
VDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISINSPALADPTLITRLADRF
ECHHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHH
GVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETLDWVEEVQKRGAGEIVLNMMN
CCEEEEEEECCCCCCCCCCEEEEEECCCCHHHEEHHHHHHHHHHHHHHCCCCCEEEEECC
QDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLEAFRDADVDGALAASVFHKQIIN
CCCCCCCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
IGELKAYLATQGVEIRIC
HHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA