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The map label for this gene is hisI

Identifier: 30063476

GI number: 30063476

Start: 2085865

End: 2086476

Strand: Direct

Name: hisI

Synonym: S2209

Alternate gene names: 30063476

Gene position: 2085865-2086476 (Clockwise)

Preceding gene: 30063475

Following gene: 30063506

Centisome position: 45.35

GC content: 52.12

Gene sequence:

>612_bases
ATGTTAACAGAACAACAACGTCGCGAACTGGACTGGGAAAAAACCGACGGTCTGATGCCGGTGATTGTGCAACACGCGGT
ATCTGGCGAAGTGTTAATGCTGGGCTACATGAACCCGGAAGCCTTAGACAAAACCATCGAAAGCGGTAAAGTCACCTTCT
TCTCGCGCACTAAACAGCGACTGTGGATCAAAGGCGAAACCTCGGGCAATTTTCTCAATGTGGTAAGTATTGCCCCGGAC
TGCGACAACGACACATTACTGGTGCTGGCGAATCCCATCGGGCCAACCTGCCACAAAGGCACCAGTAGCTGCTTCGGCAA
CACCGCTCACCAGTGGCTGTTCCTGTATCAACTGGAACAACTGCTCGCCGAACGTAAATATGCCGACCCGGAAACCTCCT
ACACCGCCAAACTGTATGCCAGCGGCACTAAACGCATTGCGCAGAAAGTGGGCGAAGAAGGCGTGGAAACCGCACTGGCA
GCAACGGTACATGATCGCTTTGAGCTGACCAACGAGGCATCTGACTTGATGTATCACCTGCTGGTGCTGTTGCAGGATCA
GGATCTGGATTTAACGACAGTAATTGAGAACCTGCATAAACGGCATCAGTGA

Upstream 100 bases:

>100_bases
ACGTTGACGGTGCGCTGGCAGCTTCCGTATTCCACAAACAAATAATCAATATTGGCGAATTAAAAGCGTACCTGGCAACA
CAGGGCGTGGAGATCAGGAT

Downstream 100 bases:

>100_bases
GTTACGGGCTGAGCAGGTGTGATGTTGTTGTCACACCCGGAGCCATTGCCCGATGCGCAAACTTATCGTGCCTACACATT
ATCTCAACACACGTAGGCCG

Product: bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein

Products: NA

Alternate protein names: Phosphoribosyl-AMP cyclohydrolase; PRA-CH; Phosphoribosyl-ATP pyrophosphatase; PRA-PH [H]

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDKTIESGKVTFFSRTKQRLWIKGETSGNFLNVVSIAPD
CDNDTLLVLANPIGPTCHKGTSSCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ

Sequences:

>Translated_203_residues
MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDKTIESGKVTFFSRTKQRLWIKGETSGNFLNVVSIAPD
CDNDTLLVLANPIGPTCHKGTSSCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ
>Mature_203_residues
MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDKTIESGKVTFFSRTKQRLWIKGETSGNFLNVVSIAPD
CDNDTLLVLANPIGPTCHKGTSSCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ

Specific function: Histidine biosynthesis; second step. Histidine biosynthesis; third step. [C]

COG id: COG0139

COG function: function code E; Phosphoribosyl-AMP cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PRA-PH family [H]

Homologues:

Organism=Escherichia coli, GI2367128, Length=203, Percent_Identity=97.0443349753695, Blast_Score=410, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6319819, Length=204, Percent_Identity=35.7843137254902, Blast_Score=102, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023019
- InterPro:   IPR008179
- InterPro:   IPR021130
- InterPro:   IPR002496
- ProDom:   PD002610 [H]

Pfam domain/function: PF01502 PRA-CH; PF01503 PRA-PH [H]

EC number: =3.5.4.19; =3.6.1.31 [H]

Molecular weight: Translated: 22882; Mature: 22882

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDKTIESGKVTFFSRTKQR
CCCHHHHHCCCCHHCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCEEEEECCCEE
LWIKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTSSCFGNTAHQWLFLYQLEQ
EEEEECCCCCEEEEEEECCCCCCCEEEEEECCCCCCCCCCCHHHHCCHHHHHHHHHHHHH
LLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALAATVHDRFELTNEASDLMYHL
HHHHHCCCCCCCCEEEEEEHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
LVLLQDQDLDLTTVIENLHKRHQ
HHHHCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDKTIESGKVTFFSRTKQR
CCCHHHHHCCCCHHCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCEEEEECCCEE
LWIKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTSSCFGNTAHQWLFLYQLEQ
EEEEECCCCCEEEEEEECCCCCCCEEEEEECCCCCCCCCCCHHHHCCHHHHHHHHHHHHH
LLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALAATVHDRFELTNEASDLMYHL
HHHHHCCCCCCCCEEEEEEHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
LVLLQDQDLDLTTVIENLHKRHQ
HHHHCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10222209; 11206551; 11258796 [H]