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The map label for this gene is yecK

Identifier: 30063321

GI number: 30063321

Start: 1927192

End: 1928292

Strand: Reverse

Name: yecK

Synonym: S2004

Alternate gene names: 30063321

Gene position: 1928292-1927192 (Counterclockwise)

Preceding gene: 30063322

Following gene: 30063320

Centisome position: 41.93

GC content: 47.68

Gene sequence:

>1101_bases
ATGCGAGGGAAAAAACGCATTGGGTTATTGTTTTTGCTGATAGCGGTTGTGGTTGGTGGCGGCGGGTTATTGCTGGCGCA
AAAAGCCTTACATAAAACGTCGGATACAGCATTTTGCCTTTCCTGCCACTCGATGAGTAAACCTTTTGAGGAATATCAGG
GAACTGTCCACTTTTCGAACCAGAAAGGGATACGTGCGGAATGTGCCGATTGCCATATTCCAAAGTCAGGGATGGATTAT
TTATTTGCTAAATTAAAAGCATCTAAAGATATTTATCATGAATTTGTTAGCGGCAAAATAGACAGTGACGATAAGTTCGA
AACTCATCGCCAGGAAATGGCCGAAACAGTATGGAAAGAATTAAAAGCAACTGACTCTGCAACGTGCCGTAGTTGCCATT
CTTTTGATGCCATGGATATTGCCTCGCAAAGTGAATCTGCGCAGAAAATGCATAACAAAGCACAAAAGGGCGGCGAAACC
TGTATCGATTGTCATAAAGGCATTGCCCATTTTCCGCCAGAAATAAAAATGGATGACAACGCGGCGCATGAGCTGGAAAG
TCAGGCCGCTACTTCAGTGACTAATGGCGCACATATTTATCCTTTCAAAACTTCTCGCATAGGCGAGCTGGCTACCGTGA
ATCCTGGTACCGATCTCACCGTCGTTGATGCCAGTGGCAAACAGCCGATCGTTCTGTTGCAGGGTTATCAAATGCAGGGC
AGTGAAAACACGCTCTACCTGGCGGCAGGTCAACGGCTGGCGCTAGCCACATTAAGTGAAGAAGGTATCAAGGCGCTCAC
GGTAAACGGGGAATGGCAGGCTGACGAATACGGCAATCAATGGCGTCAGGCGTCTTTACAGGGTGCGCTTACCGATCCCG
CATTAGCGGACCGTAAACCGCTATGGCAATACGCTGAAAAACTTGACGATACCTATTGCGCTGGTTGTCATGCCTCTATT
GCCGCCGACCATTACACCGTCAATGCGTGGCCGTCCATTGCCAAAGGAATGGGGGCACGAACCAGCATGAGCGAAAACGA
ACCGGACATTTTAACGCGGTATTTCCAGTACAACGCCAAAGATATTACCGAGAAACAGTGA

Upstream 100 bases:

>100_bases
TATTTCAGGCAGTGTTGTGCCGAGTTATGCAAGCGGTTAATTCGTTGTGTATTTAATTATACAATGATTTCGGTGTTCTG
GAATTTATTTAGAGGAATCT

Downstream 100 bases:

>100_bases
TCCTCGTTGATCAGGGAGGAAGTTATGGCATTAACAAGACGTGAATTTATTAAACACAGCGGTATTGCCGCTGGAGCGCT
GGTGGTGACATCGGCAGCAC

Product: putative cytochrome C-type protein

Products: H2O; trimethylamine; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHSMSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDY
LFAKLKASKDIYHEFVSGKIDSDDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTDLTVVDASGKQPIVLLQGYQMQG
SENTLYLAAGQRLALATLSEEGIKALTVNGEWQADEYGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASI
AADHYTVNAWPSIAKGMGARTSMSENEPDILTRYFQYNAKDITEKQ

Sequences:

>Translated_366_residues
MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHSMSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDY
LFAKLKASKDIYHEFVSGKIDSDDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTDLTVVDASGKQPIVLLQGYQMQG
SENTLYLAAGQRLALATLSEEGIKALTVNGEWQADEYGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASI
AADHYTVNAWPSIAKGMGARTSMSENEPDILTRYFQYNAKDITEKQ
>Mature_366_residues
MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHSMSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDY
LFAKLKASKDIYHEFVSGKIDSDDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTDLTVVDASGKQPIVLLQGYQMQG
SENTLYLAAGQRLALATLSEEGIKALTVNGEWQADEYGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASI
AADHYTVNAWPSIAKGMGARTSMSENEPDILTRYFQYNAKDITEKQ

Specific function: Part of the anaerobic respiratory chain of trimethylamine-N-oxide reductase TorZ. Required for electron transfer to the TorZ terminal enzyme [H]

COG id: COG3005

COG function: function code C; Nitrate/TMAO reductases, membrane-bound tetraheme cytochrome c subunit

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TorC/TorY family [H]

Homologues:

Organism=Escherichia coli, GI1788181, Length=366, Percent_Identity=98.0874316939891, Blast_Score=752, Evalue=0.0,
Organism=Escherichia coli, GI1787230, Length=374, Percent_Identity=33.4224598930481, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1788530, Length=180, Percent_Identity=42.7777777777778, Blast_Score=157, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009154
- InterPro:   IPR011031
- InterPro:   IPR005126 [H]

Pfam domain/function: PF03264 Cytochrom_NNT [H]

EC number: NA

Molecular weight: Translated: 40194; Mature: 40194

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHSMSKPFEEYQGTVHFSN
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEHHHHCCCHHHHCCEEEECC
QKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDSDDKFETHRQEMAETVWKE
CCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
LKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGETCIDCHKGIAHFPPEIKMDDN
HHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHCCCCCEECCC
AAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTDLTVVDASGKQPIVLLQGYQMQG
HHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEECCCCCEEEEECCCCCCEEEEECEEECC
SENTLYLAAGQRLALATLSEEGIKALTVNGEWQADEYGNQWRQASLQGALTDPALADRKP
CCCEEEEECCCEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCH
LWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIAKGMGARTSMSENEPDILTRYFQYNAK
HHHHHHHHCCHHHCCCCHHHHCCCEEECCCHHHHHCCCCCCCCCCCCHHHHHHHHHCCCC
DITEKQ
CCCCCC
>Mature Secondary Structure
MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHSMSKPFEEYQGTVHFSN
CCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEHHHHCCCHHHHCCEEEECC
QKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDSDDKFETHRQEMAETVWKE
CCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
LKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGETCIDCHKGIAHFPPEIKMDDN
HHCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHCCCCCEECCC
AAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTDLTVVDASGKQPIVLLQGYQMQG
HHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEECCCCCEEEEECCCCCCEEEEECEEECC
SENTLYLAAGQRLALATLSEEGIKALTVNGEWQADEYGNQWRQASLQGALTDPALADRKP
CCCEEEEECCCEEEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCH
LWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIAKGMGARTSMSENEPDILTRYFQYNAK
HHHHHHHHCCHHHCCCCHHHHCCCEEECCCHHHHHCCCCCCCCCCCCHHHHHHHHHCCCC
DITEKQ
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: trimethylamine-N-oxide; NADH [C]

Specific reaction: trimethylamine-N-oxide + NADH = H2O + trimethylamine + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]