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The map label for this gene is sdaA

Identifier: 30062932

GI number: 30062932

Start: 1488922

End: 1490286

Strand: Reverse

Name: sdaA

Synonym: S1529

Alternate gene names: 30062932

Gene position: 1490286-1488922 (Counterclockwise)

Preceding gene: 30062933

Following gene: 30062931

Centisome position: 32.4

GC content: 53.77

Gene sequence:

>1365_bases
GTGATTAGTCTATTCGACATGTTTAAGGTGGGGATTGGTCCCTCATCTTCCCATACCGTAGGGCCTATGAAGGCGGGTAA
ACAGTTCGTCGATGATCTGGTCGAAAAAGGCTTACTGGATAGCGTTACTCGCGTTGCCGTGGACGTTTATGGTTCACTGT
CGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATC
GACAGTATTCCCGGTTTTATTCGCGACGTAAAAGAGCGCGAACGTCTGCTGCTGGCACAGGGACGGCATGAAGTGGATTT
CCCGCGCGACAACGGGATGCGTTTTCATAACGGCAACCTGCCGCTGCATGAAAACGGTATGCAAATCCACGCCTATAACG
GCGATGAAGTCGTCTACAGCAAAACTTATTATTCCATCGGCGGCGGTTTTATCGTCGATGAAGAACACTTTGGTCAGGAT
GCTGCCAACGAAGTGAGCGTGCCGTATCCGTTCAAATCTGCCACCGAACTGCTCGCGTACTGTAATGAAACCGGCTATTC
GCTGTCTGGTCTCGCTATGCAGAACGAACTGGCGCTGCACAGCAAGAAAGAGATCGACGAGTATTTCGCGCATGTCTGGC
AAACCATGCAGGCATGTATCGATCGCGGGATGAACACCGAAGGTGTACTGCCAGGCCCGCTGCGCGTGCCACGTCGTGCG
TCTGCCCTGCGCCGGATGTTAGTTTCCAGCGATAAACTGTCTAACGATCCGATGAATGTCATTGACTGGGTAAACATGTT
TGCGCTGGCAGTTAACGAAGAAAACGCCGCCGGTGGTCGTGTGGTAACTGCGCCAACCAACGGTGCCTGCGGTATCGTTC
CGGCAGTGCTGGCTTACTATGACCACTTTATTGAATCGGTCAGCCCAGACATCTATACCCGTTACTTTATGGCAGCGGGC
GCGATTGGTGCATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTG
TTCAATGGCTGCTGCGGGTCTTGCAGAACTGCTGGGCGGTAGCCCGGAACAGGTTTGCGTGGCGGCGGAAATTGGCATGG
AACACAACCTTGGTTTAACCTGCGACCCGGTTGCAGGGCAGGTTCAGGTGCCGTGCATTGAGCGTAATGCCATTGCCTCT
GTGAAGGCGATTAACGCCGCACGGATGGCTCTGCGCCGCACCAGTGCACCGCGCGTCTCGCTGGATAAGGTCATCGAAAC
GATGTACGAAACCGGTAAGGACATGAACGCCAAATACCGCGAAACCTCACGCGGTGGTCTGGCAATCAAAGTCCAGTGTG
ACTAA

Upstream 100 bases:

>100_bases
TTCATGTGAATAGTTAAGCCAGTCGCCGCGTTCCCTCTTACACTATGCGCTGTTATTAGTTCGTTACTGGAAGTCCAGTC
ACCTTGTCAGGAGTATTATC

Downstream 100 bases:

>100_bases
TACTTCTTACTCGCCCATCTGCAACGGATGGGCGAATTTATACCCGCTTTCTCGTCTGCTGTAATATTCCCCACTACACT
TCCACTGTTGCGTCAGGCGT

Product: L-serine deaminase

Products: NA

Alternate protein names: SDH 1; L-serine deaminase 1; L-SD1 [H]

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTRVAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDI
DSIPGFIRDVKERERLLLAQGRHEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQACIDRGMNTEGVLPGPLRVPRRA
SALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENAAGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAG
AIGALYKMNASISGAEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPCIERNAIAS
VKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIKVQCD

Sequences:

>Translated_454_residues
MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTRVAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDI
DSIPGFIRDVKERERLLLAQGRHEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQACIDRGMNTEGVLPGPLRVPRRA
SALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENAAGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAG
AIGALYKMNASISGAEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPCIERNAIAS
VKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIKVQCD
>Mature_454_residues
MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTRVAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDI
DSIPGFIRDVKERERLLLAQGRHEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQACIDRGMNTEGVLPGPLRVPRRA
SALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENAAGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAG
AIGALYKMNASISGAEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPCIERNAIAS
VKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIKVQCD

Specific function: Deaminates also threonine, particularly when it is present in high concentration [H]

COG id: COG1760

COG function: function code E; L-serine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-sulfur dependent L-serine dehydratase family [H]

Homologues:

Organism=Escherichia coli, GI1788116, Length=454, Percent_Identity=99.7797356828194, Blast_Score=939, Evalue=0.0,
Organism=Escherichia coli, GI1789161, Length=455, Percent_Identity=77.3626373626374, Blast_Score=736, Evalue=0.0,
Organism=Escherichia coli, GI48994925, Length=453, Percent_Identity=73.9514348785872, Blast_Score=681, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004644
- InterPro:   IPR005130
- InterPro:   IPR005131 [H]

Pfam domain/function: PF03313 SDH_alpha; PF03315 SDH_beta [H]

EC number: =4.3.1.17 [H]

Molecular weight: Translated: 48906; Mature: 48906

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTRVAVDVYGSLSLTGKGH
CCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCC
HTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGRHEVDFPRDNGMRFHNGNL
CCEEEEEEEECCCCCCEEEHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCC
PLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQDAANEVSVPYPFKSATELLAY
CEECCCCEEEEECCCEEEEEEEEEECCCCEEECHHHCCCCCCCCCCCCCCCHHHHHHHHH
CNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQACIDRGMNTEGVLPGPLRVPRRA
HCCCCCCEECEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
SALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENAAGGRVVTAPTNGACGIVPAVLAYY
HHHHHHHHHCCCCCCCCHHHHHHHHHHEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHH
DHFIESVSPDIYTRYFMAAGAIGALYKMNASISGAEVGCQGEVGVACSMAAAGLAELLGG
HHHHHHCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
SPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPCIERNAIASVKAINAARMALRRTSAPRVS
CCHHEEEEEECCCCCCCCEEECCCCCCEECCEECCCHHHHHHHHHHHHHHHHHCCCCCCC
LDKVIETMYETGKDMNAKYRETSRGGLAIKVQCD
HHHHHHHHHHCCCCCCCHHHCCCCCCEEEEEEEC
>Mature Secondary Structure
MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTRVAVDVYGSLSLTGKGH
CCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCC
HTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGRHEVDFPRDNGMRFHNGNL
CCEEEEEEEECCCCCCEEEHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCC
PLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQDAANEVSVPYPFKSATELLAY
CEECCCCEEEEECCCEEEEEEEEEECCCCEEECHHHCCCCCCCCCCCCCCCHHHHHHHHH
CNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQACIDRGMNTEGVLPGPLRVPRRA
HCCCCCCEECEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
SALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENAAGGRVVTAPTNGACGIVPAVLAYY
HHHHHHHHHCCCCCCCCHHHHHHHHHHEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHH
DHFIESVSPDIYTRYFMAAGAIGALYKMNASISGAEVGCQGEVGVACSMAAAGLAELLGG
HHHHHHCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCC
SPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPCIERNAIASVKAINAARMALRRTSAPRVS
CCHHEEEEEECCCCCCCCEEECCCCCCEECCEECCCHHHHHHHHHHHHHHHHHCCCCCCC
LDKVIETMYETGKDMNAKYRETSRGGLAIKVQCD
HHHHHHHHHHCCCCCCCHHHCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2504697; 8436113; 9097040; 9278503 [H]