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The map label for this gene is cof

Identifier: 30061947

GI number: 30061947

Start: 402293

End: 403123

Strand: Direct

Name: cof

Synonym: S0397

Alternate gene names: 30061947

Gene position: 402293-403123 (Clockwise)

Preceding gene: 30061945

Following gene: 30061948

Centisome position: 8.75

GC content: 51.26

Gene sequence:

>831_bases
GTGGAGAAAGAAATGGCTCGTCTGGTAGCATTTGATATGGATGGCACTTTATTGATGCCTGACCATCATTTAGGTGAGAA
AACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGC
AGCATATTCTCGGGGCGCTATCGCTGGATGCATATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAA
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCA
TATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGAGCCCTGCGTTGTTGCAGGTATTTGTCTATAGCGGTTTTCGTT
ATCAGATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACA
CGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCT
GCCGGTGGGCTGCAATAAAGGCGCTGCATTGACGGTGCGGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATGGCCT
TTGGTGATGCGATGAACGATCGCGAAATGTTAGGCAGCGTCGGTAGCGGGTTTATTATGGGCAATGCGATGCCGCAACTG
CGCGCGGAGCTCCCGCATTTACCGGTGATTGGACATTGCCGAAATCAGGCTGTCTCTCACTATTTGACGCACTGGCTGGA
CTATCCACATCTACCTTATTCCCCCGAATAA

Upstream 100 bases:

>100_bases
TGAGCAATCGCATATAAACCCGGAACAATATTATTTAACTATTCACTATTACTTCCGTATATATCAGGTGATACTCAATC
ACCATTAACCGTGTCACAGA

Downstream 100 bases:

>100_bases
CGAGATCCCTTCCAGCACCGGGCAATTGCCCGGTTTTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACC
GCACAGAATAAATTGTCGCG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MEKEMARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGRHALEMQHILGALSLDAYLITGNGTRVHSLEGE
LLHRDDLPADVAELVLYQQWDTRASMHIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLT
RLQIQLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDREMLGSVGSGFIMGNAMPQL
RAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE

Sequences:

>Translated_276_residues
MEKEMARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGRHALEMQHILGALSLDAYLITGNGTRVHSLEGE
LLHRDDLPADVAELVLYQQWDTRASMHIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLT
RLQIQLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDREMLGSVGSGFIMGNAMPQL
RAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE
>Mature_276_residues
MEKEMARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGRHALEMQHILGALSLDAYLITGNGTRVHSLEGE
LLHRDDLPADVAELVLYQQWDTRASMHIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLT
RLQIQLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDREMLGSVGSGFIMGNAMPQL
RAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE

Specific function: Catalyzes the hydrolysis of 4-amino-2-methyl-5- hydroxymethylpyrimidine pyrophosphate (HMP-PP) to 4-amino-2- methyl-5-hydroxymethylpyrimidine phosphate (HMP-P) [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI87081741, Length=272, Percent_Identity=98.1617647058823, Blast_Score=552, Evalue=1e-158,
Organism=Escherichia coli, GI48994981, Length=260, Percent_Identity=39.2307692307692, Blast_Score=180, Evalue=9e-47,
Organism=Escherichia coli, GI1786982, Length=286, Percent_Identity=27.972027972028, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI2367265, Length=243, Percent_Identity=25.9259259259259, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR013200
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF08282 Hydrolase_3 [H]

EC number: NA

Molecular weight: Translated: 30920; Mature: 30920

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS01228 COF_1 ; PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKEMARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGRHALEMQHILGAL
CCHHHEEHEEECCCCEEECCCCCCCHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHH
SLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASMHIFNDDGWFTGKES
CCCEEEEECCCCEEEECCCCEEECCCCCHHHHHHHHHHCCCCCCEEEEECCCCEECCCCC
PALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQIQLYEALGERAHLCFSA
CCEEEEEECCCCEEEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCHHEEEEEH
TDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDREMLGSVGSGFIMGNAMPQL
HHHHHHEECCCCCCCEEEEEHHHCCCCHHHHHHHHCCCCCHHHHHCCCCCEEECCCCHHH
RAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE
HHCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MEKEMARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGRHALEMQHILGAL
CCHHHEEHEEECCCCEEECCCCCCCHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHH
SLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASMHIFNDDGWFTGKES
CCCEEEEECCCCEEEECCCCEEECCCCCHHHHHHHHHHCCCCCCEEEEECCCCEECCCCC
PALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQIQLYEALGERAHLCFSA
CCEEEEEECCCCEEEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCHHEEEEEH
TDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDREMLGSVGSGFIMGNAMPQL
HHHHHHEECCCCCCCEEEEEHHHCCCCHHHHHHHHCCCCCHHHHHCCCCCEEECCCCHHH
RAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE
HHCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA