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The map label for this gene is queA

Identifier: 30061900

GI number: 30061900

Start: 353645

End: 354715

Strand: Direct

Name: queA

Synonym: S0350

Alternate gene names: 30061900

Gene position: 353645-354715 (Clockwise)

Preceding gene: 30061899

Following gene: 30061901

Centisome position: 7.69

GC content: 53.97

Gene sequence:

>1071_bases
ATGCGCGTTACCGATTTCTCCTTTGAATTGCCCGAATCCCTGATTGCCCACTATCCCATGCCTGAACGCAGTAGCTGTCG
TTTACTGTCGCTGGACGGGCCGACGGGCGCGCTGACGCACGGTACTTTCACCGATTTACTCGATAAGCTCAACCCCGGCG
ATCTTCTGGTTTTTAATAATACCCGCGTGATCCCGGCGCGTCTGTTTGGGCGTAAAGCCAGCGGCGGCAAGATTGAAGTG
CTGGTTGAGCGGATGCTCGACGACAAACGCATTCTTGCGCATATTCGCGCCTCGAAAGCGCCAAAACCTGGCGCAGAACT
GTTGCTGGGCGATGACGAAAGTATCAACGCAACAATGACCGCGCGCCACGGCGCACTGTTTGAAGTCGAATTTAATGATG
AACGCTCGGTACTGGATATCCTCAACAGCATCGGCCATATGCCGCTGCCGCCGTATATCGATCGTCCGGACGAAGACGCT
GACCGCGAACTTTATCAAACCGTTTATAGCGAAAAACCGGGCGCGGTTGCAGCCCCGACCGCTGGTCTGCATTTTGACGA
GCCTTTGCTGGAAAAATTACGCGCCAAAGGCGTGGAGATGGCGTTTGTGACGTTGCACGTTGGTGCGGGCACCTTCCAGC
CGGTGCGCGTCGACACCATTGAAGATCACATCATGCACTCGGAATACGCTGAAGTACCGCAGGATGTGGTAGACGCGGTA
CTGGCGGCGAAAGCGCGCGGTAACCGGGTGATTGCGGTTGGCACCACTTCAGTACGTTCGCTGGAAAGCGCGGCACAGGC
GGCAAAAAACGATCTTATCGAACCGTTCTTTGACGATACCCAAATCTTTATCTATCCGGGCTTCCAGTACAAAGTGGTCG
ATGCGCTGGTGACGAACTTCCACTTGCCAGAGTCGACGCTGATTATGCTGGTTTCGGCCTTTGCCGGTTATCAACACACC
ATGAATGCCTATAAAGCGGCGGTAGAAGAGAAATATCGCTTTTTTAGTTACGGTGATGCGATGTTTATCACGTACAATCC
GCAGGCAATTAATGAGCGCGTCGGGGAGTAA

Upstream 100 bases:

>100_bases
AAATTCATCGATTATATTCTATCCAAAGGGGGGTAAAGGTTGCAGGGAGAGCGCCCCGGCACTAGACTACCCGACTCTTA
TTTTAGTCTGAGTCAGTGTC

Downstream 100 bases:

>100_bases
TTCCGCGGCGCTGGTTTAAACGTTGGACTGTTTTTCTGACGTAGTGGAGAAAAAATGAAATTTGAACTGGACACCACCGA
CGGTCGCGCACGCCGTGGCC

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFTDLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEV
LVERMLDDKRILAHIRASKAPKPGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVDTIEDHIMHSEYAEVPQDVVDAV
LAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPFFDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHT
MNAYKAAVEEKYRFFSYGDAMFITYNPQAINERVGE

Sequences:

>Translated_356_residues
MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFTDLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEV
LVERMLDDKRILAHIRASKAPKPGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVDTIEDHIMHSEYAEVPQDVVDAV
LAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPFFDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHT
MNAYKAAVEEKYRFFSYGDAMFITYNPQAINERVGE
>Mature_356_residues
MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFTDLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEV
LVERMLDDKRILAHIRASKAPKPGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVDTIEDHIMHSEYAEVPQDVVDAV
LAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPFFDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHT
MNAYKAAVEEKYRFFSYGDAMFITYNPQAINERVGE

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=356, Percent_Identity=100, Blast_Score=729, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39431; Mature: 39431

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFTDLLDKLNPGDLLVFNN
CCCCCCCCCCCHHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCCEEEECC
TRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPKPGAELLLGDDESINATMT
CEEEEHHHHCCCCCCCHHHHHHHHHHCHHHHHHHHHHCCCCCCCCEEEECCCCCCCEEEE
ARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDADRELYQTVYSEKPGAVAAPT
ECCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEECCC
AGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVDTIEDHIMHSEYAEVPQDVVDAV
CCCCCCHHHHHHHHHCCCEEEEEEEEECCCCCCCEEECHHHHHHHHHHHHHCHHHHHHHH
LAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPFFDDTQIFIYPGFQYKVVDALVTNF
HHHHCCCCEEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHC
HLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRFFSYGDAMFITYNPQAINERVGE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCEEEEEECCHHHHHHCCC
>Mature Secondary Structure
MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFTDLLDKLNPGDLLVFNN
CCCCCCCCCCCHHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCCEEEECC
TRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPKPGAELLLGDDESINATMT
CEEEEHHHHCCCCCCCHHHHHHHHHHCHHHHHHHHHHCCCCCCCCEEEECCCCCCCEEEE
ARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDADRELYQTVYSEKPGAVAAPT
ECCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEECCC
AGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVDTIEDHIMHSEYAEVPQDVVDAV
CCCCCCHHHHHHHHHCCCEEEEEEEEECCCCCCCEEECHHHHHHHHHHHHHCHHHHHHHH
LAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPFFDDTQIFIYPGFQYKVVDALVTNF
HHHHCCCCEEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHC
HLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRFFSYGDAMFITYNPQAINERVGE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCEEEEEECCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA