Click here to switch to the map view.

The map label for this gene is tgt

Identifier: 30061901

GI number: 30061901

Start: 354770

End: 355897

Strand: Direct

Name: tgt

Synonym: S0351

Alternate gene names: 30061901

Gene position: 354770-355897 (Clockwise)

Preceding gene: 30061900

Following gene: 30061902

Centisome position: 7.71

GC content: 50.71

Gene sequence:

>1128_bases
ATGAAATTTGAACTGGACACCACCGACGGTCGCGCACGCCGTGGCCGCCTGGTCTTTGATCGTGGCGTAGTGGAAACGCC
TTGTTTTATGCCTGTTGGCACCTACGGCACCGTAAAAGGGATGACGCCGGAAGAAGTTGAAGCCACTGGCGCGCAAATTA
TCCTCGGCAACACCTTCCACCTGTGGCTGCGCCCGGGCCAGGAAATCATGAAACTGCACGGCGATCTGCACGATTTTATG
CAGTGGAAAGGACCGATTCTTACCGACTCCGGCGGCTTCCAGGTCTTCAGCCTTGGTGATATTCGTAAAATCACCGAACA
GGGCGTTCACTTCCGTAACCCGATCAACGGCGACCCGATTTTCCTCGACCCGGAAAAGTCGATGGAGATTCAGTACGATC
TTGGTTCGGATATCGTCATGATCTTTGATGAGTGTACGCCGTATCCTGCTGACTGGGATTACGCAAAACGCTCTATGGAG
ATGTCTCTGCGTTGGGCGAAGCGTAGCCGTGAGCGTTTTGACAGTCTTGGTAACAAAAATGCGTTATTTGGTATAATTCA
GGGCAGTATTTACGAAGATTTACGTGATATATCTGTTAAAGGTCTGGTAGATATCGGCTTTGATGGCTACGCTGTCGGCG
GTCTGGCTGTGGGTGAGCCGAAAGCAGATATGCACCGTATTCTGGAGCATGTGTGTCCGCAAATTCCGGCAGACAAACCG
CGTTACCTGATGGGCGTTGGTAAACCGGAAGACCTGGTTGAAGGCGTACGTCGCGGTATCGATATGTTTGACTGCGTAAT
GCCAACCCGCAACGCCCGAAATGGTCATTTGTTCGTGACCGATGGCGTGGTGAAAATCCGCAATGCGAAATATAAGAGCG
ATACTGGCCCACTCGATCCTGAGTGTGATTGCTACACCTGTCGCAATTATTCACGCGCTTACTTGCATCATCTCGACCGT
TGCAACGAAATATTAGGCGCGCGACTCAACACCATTCATAACCTTCGTTACTACCAGCGTTTGATGGCGGGTTTACGCAA
GGCTATTGAAGAGGGTAAATTAGAGAGCTTCGTAACTGATTTTTACCAGCGTCAGGGGCGAGAAGTACCACCTTTGAACG
TTGATTAA

Upstream 100 bases:

>100_bases
TATCACGTACAATCCGCAGGCAATTAATGAGCGCGTCGGGGAGTAATTCCGCGGCGCTGGTTTAAACGTTGGACTGTTTT
TCTGACGTAGTGGAGAAAAA

Downstream 100 bases:

>100_bases
TATTAATAATGAGGGAAATTTAATGAGCTTTTTTATTTCTGATGCGGTAGCGGCAACGGGTGCACCGGCGCAAGGTAGCC
CGATGTCTTTGATTTTGATG

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPEEVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFM
QWKGPILTDSGGFQVFSLGDIRKITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVGEPKADMHRILEHVCPQIPADKP
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDR
CNEILGARLNTIHNLRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD

Sequences:

>Translated_375_residues
MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPEEVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFM
QWKGPILTDSGGFQVFSLGDIRKITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVGEPKADMHRILEHVCPQIPADKP
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDR
CNEILGARLNTIHNLRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD
>Mature_375_residues
MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPEEVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFM
QWKGPILTDSGGFQVFSLGDIRKITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVGEPKADMHRILEHVCPQIPADKP
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDR
CNEILGARLNTIHNLRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=364, Percent_Identity=43.4065934065934, Blast_Score=307, Evalue=1e-83,
Organism=Escherichia coli, GI1786607, Length=375, Percent_Identity=99.7333333333333, Blast_Score=778, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17544680, Length=362, Percent_Identity=41.4364640883978, Blast_Score=284, Evalue=6e-77,
Organism=Caenorhabditis elegans, GI25146502, Length=171, Percent_Identity=30.9941520467836, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI19920510, Length=369, Percent_Identity=43.9024390243902, Blast_Score=307, Evalue=6e-84,
Organism=Drosophila melanogaster, GI21355311, Length=171, Percent_Identity=30.9941520467836, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 42608; Mature: 42608

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPEEVEATGAQIILGNTFH
CEEECCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCEEEECCEEE
LWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIRKITEQGVHFRNPINGDPI
EEECCCHHHHHHHHHHHHHHHCCCCEEECCCCEEEEECCHHHHHHHCCCEECCCCCCCCE
FLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSMEMSLRWAKRSRERFDSLGNKN
EECCCCCEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVGEPKADMHRILEHVCPQIPADKP
EEEEEECCHHHHHHHHCCCCEEEEECCCCEEECCEEECCCHHHHHHHHHHHCCCCCCCCC
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGVVKIRNAKYKSDTGPLDP
CEEEECCCHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEEECCEEEEECCCCCCCCCCCCC
ECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHNLRYYQRLMAGLRKAIEEGKLESFVTD
CCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
FYQRQGREVPPLNVD
HHHHCCCCCCCCCCC
>Mature Secondary Structure
MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPEEVEATGAQIILGNTFH
CEEECCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCEEEECCEEE
LWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIRKITEQGVHFRNPINGDPI
EEECCCHHHHHHHHHHHHHHHCCCCEEECCCCEEEEECCHHHHHHHCCCEECCCCCCCCE
FLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSMEMSLRWAKRSRERFDSLGNKN
EECCCCCEEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVGEPKADMHRILEHVCPQIPADKP
EEEEEECCHHHHHHHHCCCCEEEEECCCCEEECCEEECCCHHHHHHHHHHHCCCCCCCCC
RYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGHLFVTDGVVKIRNAKYKSDTGPLDP
CEEEECCCHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEEECCEEEEECCCCCCCCCCCCC
ECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHNLRYYQRLMAGLRKAIEEGKLESFVTD
CCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHH
FYQRQGREVPPLNVD
HHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA