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The map label for this gene is yaeD

Identifier: 30061757

GI number: 30061757

Start: 213217

End: 213792

Strand: Direct

Name: yaeD

Synonym: S0193

Alternate gene names: 30061757

Gene position: 213217-213792 (Clockwise)

Preceding gene: 30061749

Following gene: 30061758

Centisome position: 4.64

GC content: 50.17

Gene sequence:

>576_bases
GTGGCGAAGAGCGTACCCGCAATTTTTCTTGACCGTGATGGCACCATTAATGTCGATCACGGCTATGTCCATGAGATCGA
CAACTTTGAATTTATCGACGGTGTTATTGACGCCATGCGCGAGCTAAAAAAAATGGGCTTTGCGCTGGTGGTAGTAACCA
ACCAGTCTGGCATTGCTCGCGGTAAATTTACCGAAGCACAGTTTGAAACGCTGACCGAGTGGATGGACTGGTCGCTGGCG
GACCGAGATGTCGATCTGGATGGTATCTATTATTGCCCGCATCATCCGCAGGGGAGTGTTGAAGAGTTTCGCCAGGTCTG
CGATTGCCGCAAACCACATCCGGGGATGCTTTTGTCAGCACGCGATTATTTGCATATTGATATGGCCGCTTCTTATATGG
TGGGCGATAAATTAGAAGATATGCAGGCAGCGGTTGCGGCGAACGTGGGAACAAAAGTGCTGGTGCGTACGGGTAAACCT
ATTACACCTGAAGCAGAAAACGCGGCAGATTGGGTGTTAAATAGCCTGGCAGACCTGCCGCAAGCGATAAAAAAGCAGCA
AAAACCGGCGCAATGA

Upstream 100 bases:

>100_bases
TCCAGACGTCTAAATCAATCAGGTTTATGCGAAGAGCACTTTCTTGCAGGTCGAAACATGCGATACTAGCGTCCCATGCC
TTATTAAGGAGCTATAAAAG

Downstream 100 bases:

>100_bases
TTAAAAGATGAACGGTTGAAATAAAAATGCATTTTTCCGCTTGTCTTCCTGAGCCGACTCCCTATAATGCGCCTCCATCG
ACACGGCGGATGTGAATCAC

Product: D,D-heptose 1,7-bisphosphate phosphatase

Products: NA

Alternate protein names: D-glycero-D-manno-heptose 1,7-bisphosphate phosphatase

Number of amino acids: Translated: 191; Mature: 190

Protein sequence:

>191_residues
MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKKMGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLA
DRDVDLDGIYYCPHHPQGSVEEFRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
ITPEAENAADWVLNSLADLPQAIKKQQKPAQ

Sequences:

>Translated_191_residues
MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKKMGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLA
DRDVDLDGIYYCPHHPQGSVEEFRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
ITPEAENAADWVLNSLADLPQAIKKQQKPAQ
>Mature_190_residues
AKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKKMGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLAD
RDVDLDGIYYCPHHPQGSVEEFRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKPI
TPEAENAADWVLNSLADLPQAIKKQQKPAQ

Specific function: Converts the D-glycero-beta-D-manno-heptose 1,7- bisphosphate intermediate into D-glycero-beta-D-manno-heptose 1- phosphate by removing the phosphate group at the C-7 position

COG id: COG0241

COG function: function code E; Histidinol phosphatase and related phosphatases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gmhB family

Homologues:

Organism=Escherichia coli, GI1786399, Length=191, Percent_Identity=100, Blast_Score=400, Evalue=1e-113,
Organism=Escherichia coli, GI87082027, Length=150, Percent_Identity=32.6666666666667, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GMHB_ECO57 (P63229)

Other databases:

- EMBL:   AB035926
- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   B85505
- PIR:   B90654
- RefSeq:   NP_285894.1
- RefSeq:   NP_308229.1
- ProteinModelPortal:   P63229
- SMR:   P63229
- EnsemblBacteria:   EBESCT00000028049
- EnsemblBacteria:   EBESCT00000057636
- GeneID:   913974
- GeneID:   956945
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0212
- KEGG:   ecs:ECs0202
- GeneTree:   EBGT00050000010149
- HOGENOM:   HBG613347
- OMA:   VVLDSIA
- ProtClustDB:   PRK08942
- BioCyc:   ECOL83334:ECS0202-MONOMER
- GO:   GO:0005737
- InterPro:   IPR023214
- InterPro:   IPR006549
- InterPro:   IPR004446
- InterPro:   IPR006543
- InterPro:   IPR013954
- Gene3D:   G3DSA:3.40.50.1000
- TIGRFAMs:   TIGR00213
- TIGRFAMs:   TIGR01662
- TIGRFAMs:   TIGR01656

Pfam domain/function: PF08645 PNK3P; SSF56784 SSF56784

EC number: 3.1.1.-

Molecular weight: Translated: 21294; Mature: 21163

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKKMGFALVVVTNQSGIAR
CCCCCCEEEECCCCEEECCCCEEECCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCC
GKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEEFRQVCDCRKPHPGMLLSA
CCCCHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHCCCCCCCEEEEC
RDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKPITPEAENAADWVLNSLADLP
CCEEEEEHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHH
QAIKKQQKPAQ
HHHHHHCCCCC
>Mature Secondary Structure 
AKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKKMGFALVVVTNQSGIAR
CCCCCEEEECCCCEEECCCCEEECCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCCCCC
GKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEEFRQVCDCRKPHPGMLLSA
CCCCHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHCCCCCCCEEEEC
RDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKPITPEAENAADWVLNSLADLP
CCEEEEEHHHHHHHCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHH
QAIKKQQKPAQ
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11108008; 11206551; 11258796