| Definition | Shigella flexneri 2a str. 2457T, complete genome. |
|---|---|
| Accession | NC_004741 |
| Length | 4,599,354 |
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The map label for this gene is dkgB
Identifier: 30061758
GI number: 30061758
Start: 219551
End: 220354
Strand: Direct
Name: dkgB
Synonym: S0200
Alternate gene names: 30061758
Gene position: 219551-220354 (Clockwise)
Preceding gene: 30061757
Following gene: 30061761
Centisome position: 4.77
GC content: 45.77
Gene sequence:
>804_bases ATGGCTATCCCTGCATTTGGTTTAGGTACTTTCCGTCTGAAAGACGACGTTGTTATTTCATCTGTGAAAACGGCGCTTGA ACTTGGTTATCGCGCAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGGCG TGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCGAGTCTGAAA GAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCACTGGCCGTCACCAAACGATGAAGTCTCTGT TGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTGAGATCGGTATTTCCAACTTCACGATCC CATTGATGGAAAAAGTGATTGCTGCTGTTGGCGCTGAAAACATCGCTACCAACCAGATTGAACTCTCTCCTTATCTGCAA AACCGTAAAGTGGTTGCCTGGGCTAAACAGCACGGTATTCATATCACTTCCTATATGACGCTGGCTTATGGTAAGGCACT GAAAGATGAGGTTATTGCTCGTATTGCAGCTAAACACAATGCGATTCCGGCACAAGTGATTCTGGCGTGGGCTATGGGGG AAGGTTACTCAGTAATTCCTTCTTCTACTAAACGTAAAAACCTGGAAAGTAATCTTAAGGCACAAAATTTACAGCTTGAT GCCGAAGATAAAAAAGCGATCGCCGCACTGGATTGCAACGACCGCCTGGTTAGCCCGGAAGGTCTGGCTCCTGAATGGGA TTAA
Upstream 100 bases:
>100_bases AGAATCGCAAAAATCCTCTGCGTTTTACGCTCTTTTCCCGCAACAGTCTGAAGCCCATAATCACCTCAGTTAACGAAAAT AGCATTAAAAGAGGCATATT
Downstream 100 bases:
>100_bases GCCTCTCTGACAGCTCCTCCGGGAGTAATGCCAGTCAGTTAAGCAACTGACTGGCTCTTTTTCGGGGCTGTGGGGTATTT CCAGGGCCTCTCCTTTACCA
Product: 2,5-diketo-D-gluconate reductase B
Products: NA
Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]
Number of amino acids: Translated: 267; Mature: 266
Protein sequence:
>267_residues MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAAVGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLK ESLQKLRTDYVDLTLIHWPSPNDEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQ NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSVIPSSTKRKNLESNLKAQNLQLD AEDKKAIAALDCNDRLVSPEGLAPEWD
Sequences:
>Translated_267_residues MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAAVGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLK ESLQKLRTDYVDLTLIHWPSPNDEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQ NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSVIPSSTKRKNLESNLKAQNLQLD AEDKKAIAALDCNDRLVSPEGLAPEWD >Mature_266_residues AIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAAVGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKE SLQKLRTDYVDLTLIHWPSPNDEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQN RKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSVIPSSTKRKNLESNLKAQNLQLDA EDKKAIAALDCNDRLVSPEGLAPEWD
Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) [H]
COG id: COG0656
COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the aldo/keto reductase family [H]
Homologues:
Organism=Homo sapiens, GI310109922, Length=275, Percent_Identity=31.6363636363636, Blast_Score=142, Evalue=3e-34, Organism=Homo sapiens, GI310109920, Length=292, Percent_Identity=30.1369863013699, Blast_Score=139, Evalue=3e-33, Organism=Homo sapiens, GI45446745, Length=292, Percent_Identity=30.1369863013699, Blast_Score=138, Evalue=4e-33, Organism=Homo sapiens, GI4503285, Length=292, Percent_Identity=30.1369863013699, Blast_Score=138, Evalue=4e-33, Organism=Homo sapiens, GI24497577, Length=289, Percent_Identity=29.757785467128, Blast_Score=137, Evalue=7e-33, Organism=Homo sapiens, GI5174391, Length=289, Percent_Identity=29.757785467128, Blast_Score=137, Evalue=7e-33, Organism=Homo sapiens, GI5453543, Length=292, Percent_Identity=29.7945205479452, Blast_Score=137, Evalue=1e-32, Organism=Homo sapiens, GI5174695, Length=293, Percent_Identity=30.7167235494881, Blast_Score=135, Evalue=3e-32, Organism=Homo sapiens, GI24497585, Length=291, Percent_Identity=29.2096219931272, Blast_Score=134, Evalue=6e-32, Organism=Homo sapiens, GI24497583, Length=297, Percent_Identity=30.3030303030303, Blast_Score=134, Evalue=7e-32, Organism=Homo sapiens, GI93277124, Length=299, Percent_Identity=30.1003344481605, Blast_Score=129, Evalue=2e-30, Organism=Homo sapiens, GI223468663, Length=285, Percent_Identity=30.1754385964912, Blast_Score=129, Evalue=2e-30, Organism=Homo sapiens, GI4502049, Length=288, Percent_Identity=32.9861111111111, Blast_Score=125, Evalue=3e-29, Organism=Homo sapiens, GI300116273, Length=269, Percent_Identity=30.1115241635688, Blast_Score=124, Evalue=1e-28, Organism=Homo sapiens, GI291291012, Length=270, Percent_Identity=28.8888888888889, Blast_Score=117, Evalue=9e-27, Organism=Homo sapiens, GI300116271, Length=278, Percent_Identity=30.2158273381295, Blast_Score=114, Evalue=8e-26, Organism=Homo sapiens, GI310109926, Length=114, Percent_Identity=37.719298245614, Blast_Score=79, Evalue=6e-15, Organism=Homo sapiens, GI207028673, Length=114, Percent_Identity=37.719298245614, Blast_Score=78, Evalue=7e-15, Organism=Homo sapiens, GI310109924, Length=155, Percent_Identity=28.3870967741935, Blast_Score=74, Evalue=1e-13, Organism=Escherichia coli, GI1786400, Length=267, Percent_Identity=98.876404494382, Blast_Score=538, Evalue=1e-154, Organism=Escherichia coli, GI87082198, Length=256, Percent_Identity=38.28125, Blast_Score=181, Evalue=4e-47, Organism=Escherichia coli, GI1788081, Length=269, Percent_Identity=28.996282527881, Blast_Score=106, Evalue=2e-24, Organism=Escherichia coli, GI48994888, Length=271, Percent_Identity=29.1512915129151, Blast_Score=77, Evalue=1e-15, Organism=Escherichia coli, GI1787674, Length=257, Percent_Identity=27.2373540856031, Blast_Score=70, Evalue=2e-13, Organism=Caenorhabditis elegans, GI17550248, Length=271, Percent_Identity=35.0553505535055, Blast_Score=150, Evalue=7e-37, Organism=Caenorhabditis elegans, GI17537075, Length=290, Percent_Identity=35.8620689655172, Blast_Score=144, Evalue=6e-35, Organism=Caenorhabditis elegans, GI17537077, Length=288, Percent_Identity=35.7638888888889, Blast_Score=142, Evalue=2e-34, Organism=Caenorhabditis elegans, GI71998625, Length=264, Percent_Identity=31.8181818181818, Blast_Score=138, Evalue=3e-33, Organism=Caenorhabditis elegans, GI17552492, Length=267, Percent_Identity=31.0861423220974, Blast_Score=134, Evalue=7e-32, Organism=Caenorhabditis elegans, GI17561298, Length=274, Percent_Identity=30.6569343065693, Blast_Score=133, Evalue=7e-32, Organism=Caenorhabditis elegans, GI17561300, Length=277, Percent_Identity=31.4079422382672, Blast_Score=129, Evalue=2e-30, Organism=Caenorhabditis elegans, GI17538386, Length=281, Percent_Identity=30.6049822064057, Blast_Score=122, Evalue=1e-28, Organism=Caenorhabditis elegans, GI17537079, Length=294, Percent_Identity=27.5510204081633, Blast_Score=119, Evalue=2e-27, Organism=Caenorhabditis elegans, GI17566692, Length=262, Percent_Identity=30.9160305343511, Blast_Score=117, Evalue=5e-27, Organism=Caenorhabditis elegans, GI17564128, Length=292, Percent_Identity=26.7123287671233, Blast_Score=115, Evalue=3e-26, Organism=Caenorhabditis elegans, GI17562292, Length=269, Percent_Identity=28.996282527881, Blast_Score=112, Evalue=2e-25, Organism=Caenorhabditis elegans, GI212645785, Length=195, Percent_Identity=27.6923076923077, Blast_Score=64, Evalue=7e-11, Organism=Saccharomyces cerevisiae, GI6324694, Length=276, Percent_Identity=36.231884057971, Blast_Score=160, Evalue=2e-40, Organism=Saccharomyces cerevisiae, GI6320576, Length=282, Percent_Identity=36.1702127659575, Blast_Score=155, Evalue=6e-39, Organism=Saccharomyces cerevisiae, GI6321896, Length=306, Percent_Identity=29.0849673202614, Blast_Score=126, Evalue=3e-30, Organism=Saccharomyces cerevisiae, GI6322556, Length=259, Percent_Identity=27.027027027027, Blast_Score=118, Evalue=8e-28, Organism=Saccharomyces cerevisiae, GI6319625, Length=275, Percent_Identity=30.5454545454545, Blast_Score=108, Evalue=1e-24, Organism=Saccharomyces cerevisiae, GI6320079, Length=271, Percent_Identity=28.7822878228782, Blast_Score=95, Evalue=1e-20, Organism=Drosophila melanogaster, GI24644950, Length=294, Percent_Identity=32.312925170068, Blast_Score=140, Evalue=6e-34, Organism=Drosophila melanogaster, GI24662789, Length=290, Percent_Identity=30.6896551724138, Blast_Score=140, Evalue=8e-34, Organism=Drosophila melanogaster, GI24657054, Length=287, Percent_Identity=31.7073170731707, Blast_Score=135, Evalue=4e-32, Organism=Drosophila melanogaster, GI221378297, Length=314, Percent_Identity=30.5732484076433, Blast_Score=133, Evalue=1e-31, Organism=Drosophila melanogaster, GI20129731, Length=286, Percent_Identity=31.4685314685315, Blast_Score=129, Evalue=1e-30, Organism=Drosophila melanogaster, GI21356425, Length=288, Percent_Identity=30.9027777777778, Blast_Score=127, Evalue=6e-30, Organism=Drosophila melanogaster, GI24663317, Length=285, Percent_Identity=29.8245614035088, Blast_Score=127, Evalue=9e-30, Organism=Drosophila melanogaster, GI24662785, Length=289, Percent_Identity=34.2560553633218, Blast_Score=124, Evalue=6e-29, Organism=Drosophila melanogaster, GI24662781, Length=289, Percent_Identity=33.5640138408304, Blast_Score=119, Evalue=1e-27, Organism=Drosophila melanogaster, GI45553081, Length=284, Percent_Identity=28.169014084507, Blast_Score=114, Evalue=9e-26, Organism=Drosophila melanogaster, GI281366140, Length=259, Percent_Identity=26.6409266409266, Blast_Score=94, Evalue=9e-20,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001395 - InterPro: IPR018170 - InterPro: IPR020471 - InterPro: IPR023210 [H]
Pfam domain/function: PF00248 Aldo_ket_red [H]
EC number: =1.1.1.274 [H]
Molecular weight: Translated: 29492; Mature: 29361
Theoretical pI: Translated: 5.71; Mature: 5.71
Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.4 %Cys (Translated Protein) 1.9 %Met (Translated Protein) 2.2 %Cys+Met (Translated Protein) 0.4 %Cys (Mature Protein) 1.5 %Met (Mature Protein) 1.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAAVGQAIAESGVPRHELY CCCCCCCCCEEEECCHHHHHHHHHHHHHCHHHCCHHHHHCCHHHHHHHHHHCCCCCCEEE ITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPNDEVSVEEFMQALLEAKKQ EEEEHHHHCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHC GLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQNRKVVAWAKQHGIHITSYMT CCEEECCCCCCCHHHHHHHHHHHCCCCCCCCEEEECHHHCCCEEEEEHHHCCEEEEHHHH LAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSVIPSSTKRKNLESNLKAQNLQLD HHHHHHHHHHHHHHHHHHCCCCCHHHHEEECCCCCCEECCCCHHHHHHHHCCCCCCEEEC AEDKKAIAALDCNDRLVSPEGLAPEWD CCCCCEEEEECCCCCEECCCCCCCCCC >Mature Secondary Structure AIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAAVGQAIAESGVPRHELY CCCCCCCCEEEECCHHHHHHHHHHHHHCHHHCCHHHHHCCHHHHHHHHHHCCCCCCEEE ITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPNDEVSVEEFMQALLEAKKQ EEEEHHHHCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHC GLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQNRKVVAWAKQHGIHITSYMT CCEEECCCCCCCHHHHHHHHHHHCCCCCCCCEEEECHHHCCCEEEEEHHHCCEEEEHHHH LAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSVIPSSTKRKNLESNLKAQNLQLD HHHHHHHHHHHHHHHHHHCCCCCHHHHEEECCCCCCEECCCCHHHHHHHHCCCCCCEEEC AEDKKAIAALDCNDRLVSPEGLAPEWD CCCCCEEEEECCCCCEECCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 11206551; 11258796; 11108008 [H]